Free radical stress induces DNA damage response in RAW264.7 macrophages during Mycobacterium smegmatis infection

Irreversible tyrosine kinase inhibitors induce the endocytosis and downregulation of ErbB2

“…The expression of ErbB2 was analyzed by quantitative real-time PCR using SYBR Green (Power-UP SYBR-Green PCR Master Mix; Applied Biosystems, USA) in Quantstudio 3 Real-time PCR system (Applied Biosystems, USA). All the reactions were performed in triplicate and glyceraldehyde-3 phosphate dehydrogenase (GAPDH) was used as endogenous control in this study and mRNA fold changes were calculated using the 2 −ΔΔCT value [ 28 ]. The specific primer sequences used for GAPDH were as follows: 5′-GTCTCCTCTGACTTCAACAGCG-3’ (Forward), and 5′-ACCACCCTGTTGCTGTAGCCAA-3’ (Reverse).…”