Combating Staphylococcus aureus biofilm formation: the inhibitory potential of tormentic acid and 23-hydroxycorosolic acid

Ghosh, Chinmoy; Das, Manash C.; Acharjee, Shukdeb; Bhattacharjee, Samadrita; Sandhu, Padmani; Kumari, Monika; Bhowmik, Joyanta; Ghosh, Ranjit; Banerjee, Birendranath; De, Utpal Chandra; Akhter, Yusuf; Bhattacharjee, Surajit

doi:10.1007/s00203-023-03762-y

Cited by 1 publication

(2 citation statements)

References 45 publications

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“…pCM29 is used to build a promoter-GFP reporter system. pRB475 and pCN51 are used to construct arlRS complementation plasmids pCN- arlRS and pRB- arlRS [ 58 , 59 ]. The temperature-sensitive plasmid pKOR1 is used for gene knockout in staphylococci [ 60 ].…”

Section: Methodsmentioning

confidence: 99%

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The Staphylococcus aureus ArlS Kinase Inhibitor Tilmicosin Has Potent Anti-Biofilm Activity in Both Static and Flow Conditions

Wang,

Bai

et al. 2024

Microorganisms

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Staphylococcus aureus can form biofilms on biotic surfaces or implanted materials, leading to biofilm-associated diseases in humans and animals that are refractory to conventional antibiotic treatment. Recent studies indicate that the unique ArlRS regulatory system in S. aureus is a promising target for screening inhibitors that may eradicate formed biofilms, retard virulence and break antimicrobial resistance. In this study, by screening in the library of FDA-approved drugs, tilmicosin was found to inhibit ArlS histidine kinase activity (IC50 = 1.09 μM). By constructing a promoter-fluorescence reporter system, we found that tilmicosin at a concentration of 0.75 μM or 1.5 μM displayed strong inhibition on the expression of the ArlRS regulon genes spx and mgrA in the S. aureus USA300 strain. Microplate assay and confocal laser scanning microscopy showed that tilmicosin at a sub-minimal inhibitory concentration (MIC) had a potent inhibitory effect on biofilms formed by multiple S. aureus strains and a strong biofilm-forming strain of S. epidermidis. In addition, tilmicosin at three-fold of MIC disrupted USA300 mature biofilms and had a strong bactericidal effect on embedded bacteria. Furthermore, in a BioFlux flow biofilm assay, tilmicosin showed potent anti-biofilm activity and synergized with oxacillin against USA300.

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Section: Methodsmentioning

confidence: 99%

“…Tryptic soy broth (Oxoid, UK) was used for the proliferation of S. aureus and S. epidermidis strains. TSB medium with 1% glucose (TSBG) was used for staphylococcal biofilm formation [ 59 , 62 ]. Luria–Bertani (LB, Oxoid, UK) medium was used for E. coli cultivation.…”

Section: Methodsmentioning

confidence: 99%

The Staphylococcus aureus ArlS Kinase Inhibitor Tilmicosin Has Potent Anti-Biofilm Activity in Both Static and Flow Conditions

Wang,

Bai

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

Combating Staphylococcus aureus biofilm formation: the inhibitory potential of tormentic acid and 23-hydroxycorosolic acid

Cited by 1 publication

References 45 publications

The Staphylococcus aureus ArlS Kinase Inhibitor Tilmicosin Has Potent Anti-Biofilm Activity in Both Static and Flow Conditions

The Staphylococcus aureus ArlS Kinase Inhibitor Tilmicosin Has Potent Anti-Biofilm Activity in Both Static and Flow Conditions

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