Free Radical Scavenging Potential of Selected Medicinal Plants: Quantitative Evaluation by a Novel Online HPTLC-DPPH Method

Abstract

Antioxidant assays of Ocimum sanctum (OS), Aegle marmelos (AM), Bacopa monnieri (BM), and Centella asiatica (CA) were carried out by adopting a rapid, simple, accurate, and precise online high-performance thin-layer chromatography method based on quantitative estimation of stoichiometric reduction of 2,2-diphenyl-1-picrylhydrazyl by free radical scavengers. The activity of the controversial source plants of Brahmi was also compared. BM exhibited the highest activity with the lowest IC 50 (0.69 µg), followed by OS (0.95 µg), CA (1.32 µg), and AM (1.54 µg). BM possessed superior scavenging potential than CA.