Free radical scavenging activity of silibinin in nitrite-induced hemoglobin oxidation and membrane fragility models

Marouf, Bushra Hassan; Zalzala, Munaf H.; Al-Khalifa, Ihab I.; Aziz, Tavga Ahmed; Hussain, Saad Abdulrahman

doi:10.1016/j.jsps.2011.03.006

Cited by 15 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of previous studies support the view that thiamine and benfotiamine can act as antoxidative and anti-inflammatory agents. Antioxidative effects of benfotiamine were demonstrated in vivo (Schmid et al, 2008;Bozic et al, 2015b) and in vivo (Wu and Ren, 2006;Marouf et al, 2011). Here we show that benfotiamine has a protective action against the harmful effects of the prooxidant compound paraquat in neuroblastoma cells.…”

Section: Discussionsupporting

confidence: 57%

Thiamine and benfotiamine prevent stress-induced suppression of hippocampal neurogenesis in mice exposed to predation without affecting brain thiamine diphosphate levels

Vignisse

Sambon

Gorlova

et al. 2017

Molecular and Cellular Neuroscience

View full text Add to dashboard Cite

Thiamine is essential for normal brain function and its deficiency causes metabolic impairment, specific lesions, oxidative damage and reduced adult hippocampal neurogenesis (AHN). Thiamine precursors with increased bioavailability, especially benfotiamine, exert neuroprotective effects not only for thiamine deficiency (TD), but also in mouse models of neurodegeneration. As it is known that AHN is impaired by stress in rodents, we exposed C57BL6/J mice to predator stress for 5 consecutive nights and studied the proliferation (number of Ki67-positive cells) and survival (number of BrdU-positive cells) of newborn immature neurons in the subgranular zone of the dentate gyrus. In stressed mice, the number of Ki67- and BrdU-positive cells was reduced compared to non-stressed animals. This reduction was prevented when the mice were treated (200mg/kg/day in drinking water for 20days) with thiamine or benfotiamine, that were recently found to prevent stress-induced behavioral changes and glycogen synthase kinase-3β (GSK-3β) upregulation in the CNS. Moreover, we show that thiamine and benfotiamine counteract stress-induced bodyweight loss and suppress stress-induced anxiety-like behavior. Both treatments induced a modest increase in the brain content of free thiamine while the level of thiamine diphosphate (ThDP) remained unchanged, suggesting that the beneficial effects observed are not linked to the role of this coenzyme in energy metabolism. Predator stress increased hippocampal protein carbonylation, an indicator of oxidative stress. This effect was antagonized by both thiamine and benfotiamine. Moreover, using cultured mouse neuroblastoma cells, we show that in particular benfotiamine protects against paraquat-induced oxidative stress. We therefore hypothesize that thiamine compounds may act by boosting anti-oxidant cellular defenses, by a mechanism that still remains to be unveiled. Our study demonstrates, for the first time, that thiamine and benfotiamine prevent stress-induced inhibition of hippocampal neurogenesis and accompanying physiological changes. The present data suggest that thiamine precursors with high bioavailability might be useful as a complementary therapy in several neuropsychiatric disorders.

show abstract

Section: Discussionsupporting

confidence: 57%

Thiamine and benfotiamine prevent stress-induced suppression of hippocampal neurogenesis in mice exposed to predation without affecting brain thiamine diphosphate levels

Vignisse

Sambon

Gorlova

et al. 2017

Molecular and Cellular Neuroscience

View full text Add to dashboard Cite

show abstract

“…The use of natural products (functional foods) has been given special attention in this regard. Recent studies have shown that various antioxidants are effective against the damaging effects of nitrite both in vivo and in vitro (Marouf et al, ). We have previously reported that NaNO 2 induces oxidative damage in human erythrocytes (Ansari et al, ).…”

Section: Resultsmentioning

confidence: 99%

Crocin protects human erythrocytes from nitrite‐induced methemoglobin formation and oxidative damage

Ansari

Ali

Mahmood

2016

Cell Biology International

View full text Add to dashboard Cite

Sodium nitrite (NaNO ) is a common contaminant of drinking water and food and feed chain. Nitrite induces oxidative damage in humans and animals. In this work, we studied the protective effect of crocin, the active constituent of Crocus sativus (saffron), on NaNO -induced oxidative damage in human erythrocytes. Changes in oxidative stress parameters following NaNO incubation of erythrocytes in presence and absence of crocin were determined. It was found that crocin pre-treatment significantly attenuated NaNO -induced oxidative damage of proteins, lipids, and plasma membrane. Crocin reduced the level of methemoglobin, the primary acute effect of nitrite intoxication. It also improved the antioxidant capacity of cells and NaNO -induced morphological changes in erythrocytes. Crocin is thus a potent protective agent against nitrite-induced cytotoxicity.

show abstract

“…In 1.5 ml freshly prepared hemolysate, 1.0 ml of different concentrations of MEHS (10–100 μg/ml) were added each time concomitantly with 0.1 ml sodium nitrite (6.0 mM) and the formation of methemoglobin (MetHb) was monitored spectrophotometrically (631 nm) at 10, 25 and 50 min interval. [ 17 18 ]…”

Section: Methodsmentioning

confidence: 99%

Amelioration of oxidative DNA damage in mouse peritoneal macrophages by Hippophae salicifolia due to its proton (H⁺) donation capability: Ex vivo and in vivo studies

et al. 2016

View full text Add to dashboard Cite

Introduction:The present study evaluates the antioxidant effect of methanol extract of Hippophae salicifolia (MEHS) bark with special emphasis on its role on oxidative DNA damage in mouse peritoneal macrophages.Material and Methods:In vitro antioxidant activity was estimated by standard antioxidant assays whereas the antioxidant activity concluded the H+ donating capacity. Mouse erythrocytes’ hemolysis and peritoneal macrophages’ DNA damage were determined spectrophotometrically. In vivo antioxidant activity of MEHS was determined in carbon tetrachloride-induced mice by studying its effect on superoxide anion production in macrophages cells, superoxide dismutase in the cell lysate, DNA damage, lipid peroxidation, and reduces glutathione.Results:The extract showed good in vitro antioxidant activities whereas the inhibitory concentrations values ranged from 5.80 to 106.5 μg/ml. MEHS significantly (P < 0.05) attenuated the oxidative DNA damage. It also attenuated the oxidative conversion of hemoglobin to methemoglobin and elevation of enzymatic and nonenzymatic antioxidant in cells.Conclusion:The result indicates MEHS has good in vitro-in vivo antioxidant property as well as the protective effect on DNA and red blood cell may be due to its H+ donating property.

show abstract

Free radical scavenging activity of silibinin in nitrite-induced hemoglobin oxidation and membrane fragility models

Cited by 15 publications

References 31 publications

Thiamine and benfotiamine prevent stress-induced suppression of hippocampal neurogenesis in mice exposed to predation without affecting brain thiamine diphosphate levels

Thiamine and benfotiamine prevent stress-induced suppression of hippocampal neurogenesis in mice exposed to predation without affecting brain thiamine diphosphate levels

Crocin protects human erythrocytes from nitrite‐induced methemoglobin formation and oxidative damage

Amelioration of oxidative DNA damage in mouse peritoneal macrophages by Hippophae salicifolia due to its proton (H⁺) donation capability: Ex vivo and in vivo studies

Contact Info

Product

Resources

About

Free radical scavenging activity of silibinin in nitrite-induced hemoglobin oxidation and membrane fragility models

Cited by 15 publications

References 31 publications

Thiamine and benfotiamine prevent stress-induced suppression of hippocampal neurogenesis in mice exposed to predation without affecting brain thiamine diphosphate levels

Thiamine and benfotiamine prevent stress-induced suppression of hippocampal neurogenesis in mice exposed to predation without affecting brain thiamine diphosphate levels

Crocin protects human erythrocytes from nitrite‐induced methemoglobin formation and oxidative damage

Amelioration of oxidative DNA damage in mouse peritoneal macrophages by Hippophae salicifolia due to its proton (H+) donation capability: Ex vivo and in vivo studies

Contact Info

Product

Resources

About

Amelioration of oxidative DNA damage in mouse peritoneal macrophages by Hippophae salicifolia due to its proton (H⁺) donation capability: Ex vivo and in vivo studies