Free Radical Scavenging Activity of Juice and Ethanol Extracts of Garcinia Mangostana L. Leaves

Abstract

The present research was conducted to assess the free radical scavenging activity of Garcinia mangostana L leaves by DPPH (1,1, diphenyl-2 picryl hydrazyl) method. Free radical scavenging activity of juice and ethanolic extract of G. mangostana leaves were determined by using spectrophotometry UV-Vis method. Juice and 96% ethanol's extract was concentrated by using rotary evaporator to get thick extract produces rendement about 2.571 % and 4.842 %. IC50 values for juice and ethanol extract was 19.372 g/ml and 1.965 g/ml, it means at that concentration inhibited 50 % of free radical DPPH scavenge. IC50 of d-α-tocopherol was 0.737 g/ml. The result showed that juice are amounted ten times less than ethanol extract. When compared with d-αtocopherol as positive control, free radical scavenging activity of juice and ethanolic extract were twenty six and three times less than d-α-tocopherol. Based on the result of anova test with significant degree 95% can be concluded that free radical scavenging activity between juice and ethanol extract were different with d-α-tocopherol.