Free Radical Oxidation and Carcinogenesis: Debatable Issues

Рахматуллина, И. Р.; Фархутдинов, Р.Г.; Пушкарев, В. А.; Данилко, К. В.; Галимова, Э. Ф.; Баймурзина, Ю. Л.; Петрова, И. В.; Мочалов, К. С.

doi:10.24060/2076-3093-2017-7-2-54-61

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…It is known that anticancer therapy is accompanied by significant side effects, which significantly affects the course, prognosis and effectiveness of treatment of diseases [31,32].…”

Section: Discussionmentioning

confidence: 99%

Emoxipine Modulates Concentration-Dependent Effects of Cytarabine and Cyclocytidine on Activation of Human T Cells

Nizheharodava

Kvasyuk

Zafranskaya

et al. 2021

JPRI

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Title: Emoxipine modulates concentration-dependent effects of cytarabine and cyclocytidine on activation of human T cells. Introduction: Both cytarabine and cyclocytidine are used in the treatment of acute myeloid leukemia. Well known that cytarabine and other related cytosine-based nucleoside analogues are being toxic to tumor cells by increasing levels of cellular oxidative stress as it could be abrogated by antioxidants. However, very little is known both about both the effects of combinations of antimetabolites with antioxidants on the cytotoxic innate and adaptive immune cells and whether lymphocytes toxicity affects its anticancer efficiency. Aim: To estimate effects of cytarabine and cyclocytidine with emoxipine on in vitro activated human T cells at concentrations reached during in vivo treatment with high doses, conventional doses and low doses. Materials and Methods: T cells derived from blood donors were activated in vitro in cell culture medium alone or supplemented with cytarabine 0.1-10.0 μM or cyclocytidine 0.1-10.0 μM. Cell characteristics were assessed by flow cytometry. Results: Only cytarabine 1.0-10.0 μM had both antiproliferative and proapoptotic effects. Additionally, these cytarabine concentrations increased the γIFN-producing by CD3+CD4+ T cells and did not affect the release of this cytokine by CD3+CD8+ T cells. In contrast, the lowest concentration (0.1 μM) did not have or showed minor antiproliferative or cytotoxic effects, did not alter the release of γIFN. Cyclocytidine did not affect viability of normal peripheral blood mononuclear cells but decreased the proliferative capacity of activated normal T cells in dose-dependent manner. Additionally, cyclocytidine altered the percentage of γIFN-producing proliferative CD3+CD8+ cytotoxic T cells for any concentration tested (0.1, 1.0, 1 and 10.0 μM) meanwhile highly suppressed the number of the whole amount of CD3+CD8+ cells and did not affect the release of cytokines by CD3+CD4+ T cells. The study of the expression of the CD107a marker showed a significant stimulating effect of 10 µm of citarabine on the activation of subpopulations of T-lymphocytes (CD3+) and cytotoxic T-lymphocytes (CD3+CD8+).

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“…It is known that anticancer therapy is accompanied by significant side effects, which significantly affects the course, prognosis and effectiveness of treatment of diseases [31,32].…”

Section: Discussionmentioning

confidence: 99%

Emoxipine Modulates Concentration-Dependent Effects of Cytarabine and Cyclocytidine on Activation of Human T Cells

Nizheharodava

Kvasyuk

Zafranskaya

et al. 2021

JPRI

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“…В водной среде он формирует амфифильный тетрамер, что придает ему свойства катионного детергента с высокой поверхностной активностью [5]. В предыдущих исследованиях описаны противовирусные, антибактериальные, противогрибковые, противопаразитарные и противоопухолевые свойства мелиттина, и на данный момент считается, что главный эффект этого вещества как неселективного цитолитического пептида заключается в физическом и химическом разрушении всех прокариотических и эукариотических клеточных мембран [6][7][8][9][10]. Мелиттин связывается с отрицательно…”

Section: Introductionunclassified

Impact of Honeybee Venom Melittin on Cell Viability of Different Prostate Cancer Lineages

Khalikov

Gromenko

Galimova

et al. 2022

Kreativnaâ hirurgiâ i onkologiâ

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Background. Melittin is a major constituent of honeybee venom and comprises a water-soluble surfactant peptide with cytolytic effects potentially applicable in anticancer therapy. We evaluated the impact of melittin from Bashkir honeybee (Apis mellifera mellifera L.) venom on cell viability of various prostate cancer lineages.Materials and methods. MTT assays with cell viability index estimation were used to evaluate the effect of melittin on cell proliferation in various-grade malignancy prostate cancer (PC) lineages, LNCaP, PC-3 and DU145.Results and discussion. Lineage DU145 revealed a low sensitivity to melittin, because a relatively high peptide concentration of 10 μg/mL had a suppressive effect on its proliferation. With PC-3 cells, a 0.1 μg/mL concentration suppressed proliferation significantly to 46.15 %, while melittin at a 10 μg/mL dose had a cytolytic effect on most cells (4.27 % viability). LNCaP cells experienced the lowest toxicity at 10 μg/mL melittin compared to PC-3 and DU145 lineages. The LNCaP, PC-3 and DU145 PC lineages demonstrated suppressed proliferation at melittin levels 0.01–100 μg/mL.Conclusion. The study reveals a significant reduction of the PC lineages viability at a minimal melittin concentration of 0.01 μg/mL, which indicates a high cytolytic activity of this peptide and renders it a candidate agent in antitumour therapy.

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Protective Effect of Polysaccharides from Tussilago farfara L. on Bone Marrow Cells and Small Intestinal Epithelium Under Conditions of Polychemotherapy Evaluated by DNA Comet Assay

et al. 2018

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Free Radical Oxidation and Carcinogenesis: Debatable Issues

Cited by 5 publications

References 29 publications

Emoxipine Modulates Concentration-Dependent Effects of Cytarabine and Cyclocytidine on Activation of Human T Cells

Emoxipine Modulates Concentration-Dependent Effects of Cytarabine and Cyclocytidine on Activation of Human T Cells

Impact of Honeybee Venom Melittin on Cell Viability of Different Prostate Cancer Lineages

Protective Effect of Polysaccharides from Tussilago farfara L. on Bone Marrow Cells and Small Intestinal Epithelium Under Conditions of Polychemotherapy Evaluated by DNA Comet Assay

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