Free Radical Lipid Oxidation and Reproductive Health of Cows

Abstract

A b s t r a c tFree radical lipid oxidation is currently considered as one of the dominant metabolic processes of physiological system functional activity. When it goes beyond regulated limits it is considered as an inductor of free radical pathology oxidative stress. In the conditions of a big dairy cattle breeding farm (Voronezh Province), specialized in Red-and-White breed, the functioning peculiarities of the peroxidation system lipid-oxidant defense in cows at the normal course of gestation and at gestosis, at the normal course of postpartum period and at puerperal endometritis, uterus subinvolution and ovarian dysfunction were studied. The state of lipid peroxidation processes and antioxidant system were evaluated by determining total lipid content, cholesterol, triglycerides, malonic dialdehyde (MDA), nitrogen oxides (NO • ), E and C vitamins concentrations, glutathione peroxidase (GPO), glutathione reductase (GR), catalase, superoxide dismustase (SOD) activity in blood. It is demonstrated that the high activity of lipid peroxidation reactions and the system of nitrogen oxide at decrease of antioxidant defense non-enzymic link capacity is the basis of gestosis and acute postnatal complications. Thus, àn increase of MDA concentration by 42.3-43.0 %, NO • by 31.9-38.0 % was observed in cows with gestosis. At mild gestosis the glutathione peroxidase activity, catalase activity and vitamin C concentration increased by 11.0 %, 14.3 %, 38.0 %, and 24.1 %, respectively, while vitamin Å concentration decreased by 11.7 % due to more consumption for neutralization of the toxic peroxidation products. At more sever pathology, the glutathione peroxidase activity and catalase activity in blood increased by 26.0 % and 17.3 %, respectively, when compared to the healthy animals, while vitamins E and C concentrations decreased by 33.3 % (ð < 0,01) and 17.2 %, respectively. As free radical oxidation intensified, an anaerobic degradation of carbohydrates was activated to supply the tissues of developing fetus with energy under oxygen deficit occurred because of violation of the blood circulation. Postpartum inflammation in the genital organs in cows developed against the background of increase of MDA concentration by 76.0 %, GPO and GR activity by 65.8 % and 14.6 %, respectively, SOD by 46.0 %, catalase by 45.7 %, 2.9 times increase of NO • concentration and reduction of vitamin E content by 35.5 %. Infertile animals with ovarian dysfunction were characterized by high activity of lipid peroxidation processes and by low level of nitrogen oxide generation. This is indicated by an increased concentration of MDA by 57.0 %, activity of GPO by 27.6 %, GR by 10.5 %, SOD by 31.9 %, catalase by 24.3 %, with a reduced content of NO • and vitamin E by 56.9 % and 31.6 %, respectively, in comparison with healthy animals. A decrease in NO • concentration in blood could result from a sharp depression of hormone synthesizing function in ovaries, and low NO • production could disturb functions of the gonads. The high level of peroxidation is...

Changes in peroxide and antioxidant status during gestation in sows predisposed to postpartum diseases

“…У свиноматок обеих групп установлено существенное увеличение содержания NO• в 10,4 и 11,0 раз, что связано с ростом активности NO-синтаз и продукции оксида азота для расслабления гладких мышц матки, торможения сокращения матки в течение беременности и снижения тонуса цервикального канала [8]. Протекторное действие обеспечивается способностью NO• увеличивать активность антиоксидантных ферментов, а также взаимодействовать с супероксиданион -радикалом и обеспечивать детоксикацию потенциально опасных активных форм кислорода [6].…”

Hormonal and Biochemical Parameters Analysis of the Yeld Cows Blood

“…In addition to a sufficient iodine intake into the body for the production of thyroid hormones, it is important to ensure an adequate selenium intake from the feed. According to the number of studies, it has been found that pathological conditions of the reproductive system develop against the background of an increased level of oxidative stress, aggravated by a lack of micronutrients included in a structure of antioxidant enzymes (Awadeh et al, 1998;Safonov et al, 2008, Safonov et al, 2014, Kireev et al, 2018.…”

The use of carotene-containing preparation in cows for the prevention of postpartum complications

“…After introducing carrots into the diet of cows, Japanese scientists noted that "feeding carrots improved the IgG1 concentration in the plasma of the cows during parturition [19]." Therefore, more biochemical and hematological studies of cattle blood are needed [20][21][22][23][24]. The biochemical and hematological studies of cow blood are widely used for predicting or diagnosing antepartum and postpartum metabolic disorders in cows [25][26][27][28].…”