Free radical equilibrium in interscapular brown adipose tissue: Relationship between metabolic profile and antioxidative defense

Petrović, Vesna; Buzadžić, Biljana; Korać, Aleksandra; Vasilijević, Ana; Janković, Aleksandra; Korać, Bato

doi:10.1016/j.cbpc.2005.10.004

Cited by 24 publications

(33 citation statements)

References 34 publications

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“…NO has been reported to regulate tissue blood flow [16], proliferation and differentiation of brown adipocytes, apoptosis [9], capillary remodeling [11] and mitochondriogenesis [13,15]. It regulates some of these pathways directly, but the targeting of some NO effects in IBAT has been found to be mediated by O 2 Á-and glutathione [10,13,14]. Thus, the enzymes involved in reactive species metabolism became an essential component of redox signaling, tightly regulating and targeting ROS and RNS production, which is crucial for their effects.…”

Section: Introductionmentioning

confidence: 99%

“…However, increase in tissue metabolic activity unavoidably leads to generation of reactive oxygen and nitrogen species (ROS and RNS, respectively), well known mediators of several forms of tissue damage. Cellular homeostasis in these conditions is maintained by an almost perfect match in the tissue antioxidative defense (AD) [5][6][7][8][9][10]. In addition, we have recently shown that the changes in AD occurring during cold-acclimation should be observed as a part of newly established IBAT homeostasis that characterizes intensive oxidative metabolism, uncoupling, lipolysis and decreased rate of apoptosis [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Cellular homeostasis in these conditions is maintained by an almost perfect match in the tissue antioxidative defense (AD) [5][6][7][8][9][10]. In addition, we have recently shown that the changes in AD occurring during cold-acclimation should be observed as a part of newly established IBAT homeostasis that characterizes intensive oxidative metabolism, uncoupling, lipolysis and decreased rate of apoptosis [9][10][11]. Therefore, shown decrease in manganese and copper, zinc superoxide dismutase (Mn-and CuZn-SOD, respectively, EC 1.15.1.1) activity in rats kept at cold for 45 days, accompanied by an increased UCP1 expression, was explained as adaptive response of enzymatic activities on decreased superoxide (O 2 Á-) production by uncoupling [9].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue

et al. 2009

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Cold-exposure activates interscapular brown adipose tissue (IBAT) non-shivering thermogenesis that relies primarily on intensification of metabolic rate and uncoupling. During cold-acclimation, uncoupling in IBAT decreases superoxide (O(2) (·-)) production and as an adaptive response the activities of manganese and copper, zinc superoxide dismutase (Mn- and CuZn-SOD, respectively) are decreased, as well. However, molecular mechanisms governing this SODs adaptive response are still unsolved. Besides, knowing that NO reinforces IBAT uncoupling, we wondered whether nitric oxide (NO) is taking part in SODs regulation? Mn- and CuZn-SOD mRNA and protein expression, uncoupling protein 1 (UCP1), nitrotyrosine and nuclear factor-kappa B (NF-κB) immunolabeling, as well as total SOD (tSOD) activity in IBAT of rats subjected to cold (4 ± 1°C) for 1, 3, 7, 12, 21 and 45 days and treated by l-arginine or N(ω)-nitro-l-arginine-methyl ester (l-NAME) were examined. Cold increased UCP1 immunopositivity and decreased tSOD activity during entire cold-acclimation and transiently, (day 3), activated NF-κB and increased Mn and CuZn-SOD mRNA expression and nitrotyrosine labeling, suggesting NO involvement in this signaling. However, SODs mRNA expression was decreasing from day 12 till the end of cold-acclimation. l-arginine augmented and prolonged cold-induced UCP1 and nitrotyrosine immunopositivity, NF-κB activation and SODs mRNA expression increase, while l-NAME expressed an opposite effect. Related to cold, l-arginine decreased, while l-NAME increased Mn-SOD protein expression. In contrast, neither low temperature nor both treatments applied affected CuZn-SOD protein expression. The results showed that adaptive decrease in SODs activity on uncoupling-decreased O(2) (·-) production was achieved already at the level of gene transcription and that NO takes part in the regulation of IBAT SOD isoforms.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue

et al. 2009

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“…Cellular homeostasis under conditions of increased reactive oxygen species production is achieved by a proportional increase in tissue antioxidative defence (AD) (Halliwell and Gutteridge, 1990;Buzadzic et al, 1997;Buzadzic et al, 1999;Korac and Buzadzic, 2001;Petrovic et al, 2006). AD consists of enzymes -copper, zinc and manganese superoxide dismutase (CuZnSOD and MnSOD, EC·1.15.1.1), catalase (CAT, EC·1.11.1.6), glutathione peroxidase (GSH-Px, EC·1.11.1.9), glutathione S-transferase (GST, EC·2.5.1.18), thioredoxin reductase (TR, EC·1.6.4.5), glutathione reductase (GR, EC·1.6.4.2) and low molecular mass antioxidants such as vitamins E and C, glutathione (GSH), etc.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative defence alterations in skeletal muscle during prolonged acclimation to cold: role ofl-arginine/NO-producing pathway

Petrović

Buzadžić

Korać

et al. 2008

Journal of Experimental Biology

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SUMMARYEarly in cold acclimation (1-7·days), heat is produced by shivering, while late in cold acclimation (12-45·days), skeletal muscle contributes to thermogenesis by tissue metabolism other than contractions. Given that both thermogenic phases augment skeletal muscle aerobic power and reactive species production, we aimed in this study to examine possible changes in skeletal muscle antioxidative defence (AD) during early and late cold acclimation with special emphasis on the influence of the Larginine/nitric oxide (NO)-producing pathway on the modulation of AD in this tissue. Adult Mill Hill hybrid hooded rat males were divided into two main groups: a control group, which was kept at room temperature (22±1°C), and a group maintained at 4±1°C for 45·days. The cold-acclimated group was divided into three subgroups: untreated, L-arginine treated and N -nitro-L-arginine methyl ester (L-NAME) treated. The AD parameters were determined in the gastrocnemius muscle on day 1, 3, 7, 12, 21 and 45 of cold acclimation. The results showed an improvement of skeletal muscle AD in both early and late cold acclimation. Clear phasedependent changes were seen only in copper, zinc superoxide dismutase activity, which was increased in early cold acclimation but returned to the control level in late acclimation. In contrast, there were no phase-dependent changes in manganese superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase, the activities of which were increased during the whole cold exposure, indicating their engagement in both thermogenic phases. L-Arginine in early cold acclimation accelerated the cold-induced AD response, while in the late phase it sustained increases achieved in the early period. L-NAME affected both early and late acclimation through attenuation and a decrease in the AD response. These data strongly suggest the involvement of the L-arginine/NO pathway in the modulation of skeletal muscle AD.

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“…The GPx, GR and GST activities were measured according to Chiu et al [50]. The CAT activity and GSH content were analyzed as described by Mourente et al [51] and Petrovi c et al [52], respectively. The intestinal protein content was determined following the method of Bradford [53], which was used to calculate the intestinal immune and antioxidant parameters.…”

Section: Sample Collection and Analysismentioning

confidence: 99%

Impact of exogenous lipase supplementation on growth, intestinal function, mucosal immune and physical barrier, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella)

Liu

Feng²,

Jiang

et al. 2016

Fish & Shellfish Immunology

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Free radical equilibrium in interscapular brown adipose tissue: Relationship between metabolic profile and antioxidative defense

Cited by 24 publications

References 34 publications

Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue

Antioxidative defense and mitochondrial thermogenic response in brown adipose tissue

Antioxidative defence alterations in skeletal muscle during prolonged acclimation to cold: role ofl-arginine/NO-producing pathway

Impact of exogenous lipase supplementation on growth, intestinal function, mucosal immune and physical barrier, and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella)

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