“…The presence of stored mRNA in dormant sclerotia was examined by measuring the content, the size, and the molecular associations exhibited by poly(A) using The presence of poly(A) alone cannot serve as conclusive evidence for the existence of stored poly(A)-containing RNA [poly(A)+RNA] molecules since free poly(A) tracts have been observed in other dormant systems [14]. In order to determine whether the sclerotial poly(A) sequences are free or associated with larger components, a post-mitochondrial supernatant was subjected to density gradient centrifugation and the gradient fractions were tested for poly(A) by hybridization with [3H]-poly(U).…”