Free or fixed state of nHAP differentially regulates hBMSC morphology and osteogenesis through the valve role of ITGA7

Bao, Fangyuan; Yi, Junzhi; Liu, Yixiao; Zhong, Yi; Zhang, Hui; Wu, Zhonglin; Heng, Boon Chin; Wang, Ying; Wang, Ziyang; Xiao, Lizi; Liu, Hua; Ouyang, Hongwei; Zhou, Jing

doi:10.1016/j.bioactmat.2022.03.016

Cited by 9 publications

(9 citation statements)

References 36 publications

(44 reference statements)

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“…The osteogenesis enhancement effect could be resulting from both biophysical cues such as the nanotopography, 43 and biochemical signals such as the enhanced ion interactions. 44 The fixed nanoapatites may improve osteogenesis via integrin-mediated upregulation of the PI3K-AKT signaling pathway, 45 and the high levels of Ca 2+ on scaffold surface may induce the Ca 2+ -mediated activation of BMP-and Wnt-signaling pathways. 46 These signaling pathways may jointly contribute to the enhanced osteogenesis on the nanoapatite-PMDE-scaffold.…”

Section: Resultsmentioning

confidence: 99%

Surface Tailoring of 3D Scaffolds to Promote Osteogenic Differentiation

Song

Zhu

Shi

et al. 2023

ACS Appl. Bio Mater.

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Customized bone scaffolds with osteogenic activities are desired for the regenerative repair of large-scale or irregularly shaped bone defects. This study developed a facile method to create osteogenic surfaces on three-dimensional (3D) printed scaffolds through coating-induced mineralization. The coating was synthesized using chemical vapor deposition of a polyelectrolyte containing oppositely charged groups. The opposite charges on the 3D scaffold played a crucial role in promoting the formation of nanoapatites without agglomeration, resulting in the retention of micro-and nanoscale pore openings needed for preosteoblasts to proliferate, differentiate, and migrate. The nanoapatite scaffold exhibited significant enhancement in osteoinductivity with a 107% increase in alkaline phosphatase expression and a 163% increase in osteocalcin activity compared to the pristine scaffold. The nanoapatite scaffold provided cues for preosteoblasts to grow along aligned features and migrate collectively. The findings of this study demonstrate the synergistic effect of oppositely charged polyelectrolytes and mineralized nanoapatites on promoting osteogenic activities on scaffold surfaces.

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Section: Resultsmentioning

confidence: 99%

Surface Tailoring of 3D Scaffolds to Promote Osteogenic Differentiation

Song

Zhu

Shi

et al. 2023

ACS Appl. Bio Mater.

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“…The images of the cellular adhesion morphology on the 1st, 3rd, and 5th days showed that the cells stretched on the surfaces and displayed a spindle shape, indicating proper cellular attachment and viability in different content of nHA microenvironments. Meanwhile, the area of BMSCs spread and stretched on the hydrogel surface in the PLGA_PVGM/D@20nHA group was larger than in the PLGA_PVGM/D@30nHA group, indicating that the reduction in cell spreading area in the PLGA_PVGM/D@30nHA group may be associated with cell differentiation [ 53 ] (Fig. 5 E, Additional file 1 : Fig.…”

Section: Results and Disscussionmentioning

confidence: 99%

Self-healing hybrid hydrogels with sustained bioactive components release for guided bone regeneration

Kong

et al. 2023

J Nanobiotechnol

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Guided bone regeneration (GBR) is widely used in treating oral bone defects to exclude the influence of non-osteogenic tissue on the bone healing process. The traditional method of GBR with a titanium mesh to treat large-area bone defects is limited by the deficiency of increased trauma and costs to patients. Herein, a bi-layered scaffold for GBR composed of a fiber barrier layer and a self-healing hydrogel repair layer is successfully fabricated. The barrier layer is a fibrous membrane material with specific porosity constructed by electrospinning, while the functional layer is a self-healing hydrogel material formed by multiple dynamic covalent bonds. The system can provide an osteogenic microenvironment by preventing the infiltration of connective tissue to bone defects, maintain the stability of the osteogenic space through the self-healing property, and regulate the release of bioactive substances in the dynamic physical condition, which is beneficial to osteoblast proliferation, differentiation, and bone regeneration. This study focused on exploring the effects of different crosslinkers and bonding methods on the comprehensive properties of hydrogels. and proved that the hybrid scaffold system has good biocompatibility, cell barrier function and can enhance bone regeneration activity. Thereby it could be a promising clinical strategy for bone regeneration.

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“…Phosphoinositide 3-kinase (PI3K)protein kinase B (AKT) pathway has been widely studied, and the activation of PI3K can upregulate AKT and consequently activate phosphorylation of mammalian target of rapamycin (mTOR) (Figure 2d), which not only participates in osteogenic differentiation but also upregulates the formation of blood vessels. [67a,70] HAp nanoparticle (NP) coating upregulated osteogenesis through the integrin 𝛼7-mediated PI3K-AKT signaling pathway, [71] and HAp nanorods promoted osteoblast differentiation by mediating mTOR signaling pathway and autophagy. [72] In addition, the topographical features of HAp including nanorod and micropattern structures stimulated osteogenesis by activat-ing the BMP/Smad pathway, [11] which in turn activated some integrin subunits and connexin protein (Cx43)-related cell-cell communication.…”

Section: Hap-based Nanostructuresmentioning

confidence: 99%

Materials‐Mediated In Situ Physical Cues for Bone Regeneration

Liu,

Zhang,

et al. 2023

Adv Funct Materials

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Physical cues like morphology, light, electric signal, mechanic signal, magnetic signal, and heat can be used as alternative regulators for expensive but short‐acting growth factors in bone tissue engineering to promote osteogenic differentiation and bone regeneration. As physical stimulation applied directly to the tissue cannot be focused on the bone defect area to regulate the cell behaviors and fate in situ, this limits the efficiency of precise bone regeneration. Biomaterials‐mediated in situ physical cues, as an effective strategy combining the synergistic effect of materials themselves, are put forward and studied widely to promote osteogenic differentiation and bone repair efficiently and precisely. Different types of physical cues provide different choices to better satisfy the requirements for targeted bone defect repair. In this review, the recent research about different biomaterials‐mediated physical cues accelerating osteogenesis in vitro and promoting in situ bone formation in vivo is introduced. Meanwhile, the corresponding possible mechanisms of various physical cues regulating cell responses are also discussed. This review provides useful and enlightening guidance for the utilization of intrinsically physical properties of functional materials to achieve efficient bone regeneration, leading to the design and construction of smart biomaterials for practical applications, and eventually promoting clinical translation.

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Free or fixed state of nHAP differentially regulates hBMSC morphology and osteogenesis through the valve role of ITGA7

Cited by 9 publications

References 36 publications

Surface Tailoring of 3D Scaffolds to Promote Osteogenic Differentiation

Surface Tailoring of 3D Scaffolds to Promote Osteogenic Differentiation

Self-healing hybrid hydrogels with sustained bioactive components release for guided bone regeneration

Materials‐Mediated In Situ Physical Cues for Bone Regeneration

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