FRAMESHIFT SUPPRESSION IN <i>SACCHAROMYCES CEREVISIAE</i> VI. COMPLETE GENETIC MAP OF TWENTY-FIVE SUPPRESSOR GENES

GCN2 is a protein kinase that phosphorylates the alpha‐subunit of translation initiation factor 2 (eIF‐2) and thereby stimulates translation of GCN4 mRNA in amino acid‐starved cells. We isolated a null mutation in a previously unidentified gene, GCN20, that suppresses the growth‐inhibitory effect of eIF‐2 alpha hyperphosphorylation catalyzed by mutationally activated forms of GCN2. The deletion of GCN20 in otherwise wild‐type strains impairs derepression of GCN4 translation and reduces the level of eIF‐2 alpha phosphorylation in vivo, showing that GCN20 is a positive effector of GCN2 kinase function. In accordance with this conclusion, GCN20 was co‐immunoprecipitated from cell extracts with GCN1, another factor required to activate GCN2, and the two proteins interacted in the yeast two‐hybrid system. We conclude that GCN1 and GCN20 are components of a protein complex that couples the kinase activity of GCN2 to the availability of amino acids. GCN20 is a member of the ATP binding cassette (ABC) family of proteins and is closely related to ABC proteins identified in Caenorhabditis elegans, rice and humans, suggesting that the function of GCN20 may be conserved among diverse eukaryotic organisms.

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GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells.

Aldana

1995

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X. Yeast sequencing reports. Revised nucleotide sequence of the cor region of yeast Saccharomyces cerevisiae chromosome X

Huang¹,

Manus²,

Chuat³

et al. 1994

Yeast

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The COR region, a gene cluster located on chromosome X of Saccharomyces cerevisiae and including genes CYC1, UTR1, UTR3, OSM1, tRNA(Gly) and RAD7, was sequenced within the framework of the European Union genome systematic sequencing project. It was compared with previously published sequences to be found in GenBank under the acronym YSCCORA. While some of the discrepancies observed can be readily ascribed to polymorphism, others most probably result from sequencing errors. A revised version of the sequence of the COR cluster is given.

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Construction of a set of convenient saccharomyces cerevisiae strains that are isogenic to S288C

Winston

Dollard

Ricupero-Hovasse

1995

Yeast

910

546

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FRAMESHIFT SUPPRESSION IN SACCHAROMYCES CEREVISIAE VI. COMPLETE GENETIC MAP OF TWENTY-FIVE SUPPRESSOR GENES

Cited by 38 publications

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GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells.

GCN20, a novel ATP binding cassette protein, and GCN1 reside in a complex that mediates activation of the eIF-2 alpha kinase GCN2 in amino acid-starved cells.

X. Yeast sequencing reports. Revised nucleotide sequence of the cor region of yeast Saccharomyces cerevisiae chromosome X

Construction of a set of convenient saccharomyces cerevisiae strains that are isogenic to S288C

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