Fractionation of Some Bovine Whey Proteins by Recycling Gel Filtration on a Large Scale

Abstract

Fractionation of bovine whey concentrate was performed by gel filtration on Sephadex G-75 both on a laboratory scale and on a large scale. By a recycling procedure and improved separation was obtained and the whey proteins were resolved into four fractions in the weight ratio 3:12:1:4. The fractions were analysed by polyacrylamide gel (PAG) electrophoresis and the apparent molecular weights were determined by thin layer gel chromatography (TLG) and by sodium dodecylsulfate (SDS) gel electrophoresis.

Membrane Chromatography and Fractionation of Proteins from Whey—A Review

“…In the dairy and biopharmaceutical industries, membrane- [17,18] and chromato graphy- [19,20] based downstream processes have already been attempted for the separation and purification of proteins from whey. In the chromatographic process, different stationary phases, such as packed bed [21,22], monolith [23,24], and fluidized or expanded bed [25,26] have been adopted for the fractionation of individual proteins from whey. In the membrane-based bioseparation process, protein molecules are separated from each other depending on the molecular weight cut-off of the membrane [27,28].…”