Fractionation of protein mixtures on columns of granulated agar

Polson, A.

doi:10.1016/0006-3002(61)90016-6

Cited by 127 publications

(13 citation statements)

References 7 publications

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“…The pore radius of agarose gels may be roughly estimated from the data for agar (Polson, 1961 ;Hjerten, 1962Hjerten, , 1964Ackers & Steere, 1962) to be 200 A in a 4 % (w/v) gel ranging up to around 1200 A in a 1% (w/v) gel. Thus influenza virus (500 A radius) behaves anomalously on 4 % (w/v) gel in not eluting at the void volume (determined by Eseherichia coli to be 34 % of total column volume, compared with a theoretical value of 27 % for tightly packed spheres).…”

Section: Discussionmentioning

confidence: 99%

A Chromatographic Procedure for the Purification of Influenza Virus

Pepper

1967

Journal of General Virology

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SUMMARYOwing to the difficulties of achieving consistently good recoveries of virus from allantoic fluid containing influenza virus by chromatography on calcium phosphate columns the factors determining optimum yield were systematically investigated. It was found that calcium phosphate prepared by precipitation at a controlled pH of 7.5 gave best results provided that eluting buffers were adjusted to the same pH. Although calcium phosphate fractions were still evidently contaminated with non-viral material, further purification could be obtained by chromatography in agarose-gel columns. It appeared that separation of the virus on these columns was probably achieved as a result of the binding of virus surface groups on to the gel. A minimum overall purification factor of about 800-fold was achieved.

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Section: Discussionmentioning

confidence: 99%

A Chromatographic Procedure for the Purification of Influenza Virus

Pepper

1967

Journal of General Virology

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“…The first agar and agarose gel particles used for chromatography were suspensions prepared by granulation of gels set in bulk (14,27). Also some early commercially available agarose gel preparations were of granular (irregular) type such as those at the time produced by Mann Research Inc., New York, USA.…”

Section: Granulationmentioning

confidence: 99%

Microcapsules for Cell Transplantation: Design, Preparation, and Application

Su¹

2013

Microspheres and Microcapsules in Biotechnology

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“…Sephadex G-75 and G-2oo were used in accordance with the maker's directions. Agar and agarose columns were at first made by Polson's (1961) method. More effective columns were later made from pearl-form agarose (Hjert6n, 1964) prepared with Emulphor EL.…”

Section: The Largest Soluble Rabies Antigen 40imentioning

confidence: 99%

The Largest Rabies-specific Antigen in Extracts of Infected Suckling Mouse Brains

Katz

Larsson

Mead

1968

Journal of General Virology

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SUMMARYExtracts of rabies-infected suckling mouse brains purified by precipitation at pH 4"5, freed from smaller antigens by sedimentation at 161, I8og and digested with RNase, DNase and trypsin show in the ultracentrifuge a component of $20 ~ I6 to I8 which is lacking in extracts of normal suckling mouse brains similarly treated. The largest rabies soluble antigen ('outer antigen': Mead, I962b) has a sedimentation constant S~0 ~ I6 estimated by the 'biological' method of Poison & van Regenmortel 0960. The purified antigen appears to consist of rings or possibly single-turn helices about lOO in diameter containing about 0"57/zg. pentose (as ribose) per/zg, total nitrogen. The antigen also appears to contain deoxypentose. It is resistant to pancreatic RNase, DNase, trypsin and chymotrypsin, has a density of about 1.34 g./cma. in CsC1 and an electrophoretic mobility about 7/8 that of rabbit serum albumin at pH 8"5.Preparative density-gradient centrifugation in the analytical rotor of the Model E Spinco centrifuge is described. This allows the method to be applied to smaller particles than can be treated in the S.W. 39 rotor.

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Fractionation of protein mixtures on columns of granulated agar

Cited by 127 publications

References 7 publications

A Chromatographic Procedure for the Purification of Influenza Virus

A Chromatographic Procedure for the Purification of Influenza Virus

Microcapsules for Cell Transplantation: Design, Preparation, and Application

The Largest Rabies-specific Antigen in Extracts of Infected Suckling Mouse Brains

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