Fractionation of ethanolic and hydroalcoholic extracts of green propolis using supercritical carbon dioxide as an anti-solvent to obtain artepillin rich-extract

Monroy, Yaneth M.; Rodrigues, Rodney Alexandre Ferreira; Rodrigues, Marili Villa Nova; Cabral, Fernando A.

doi:10.1016/j.supflu.2018.04.016

Cited by 15 publications

(11 citation statements)

References 40 publications

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“…Furthermore, the method of extraction and solvent can change the chemical composition of propolis extract [53]. The results found in this study confirm the influence of the type and origin of the raw material [54], as well as the extraction method [55], in the composition and characteristics of the extracts. Serra Bonvehí and Ventura [56] investigated fifteen propolis samples from various botanic and geographic origins, verifying significant differences in their contents of polyphenols, flavonoids and active components.…”

Section: Resultssupporting

confidence: 72%

“…Most polar phenolic compounds are practically insoluble in pure CO 2 , but are sufficiently soluble in a CO 2 +ethanol mixture or in a CO 2 +ethanol+water mixture, allowing for their separation on the basis of molecular weights and polarity. Monroy et al [55] used green propolis from southeastern Brazil to obtain extracts concentrated in phenolic compounds using supercritical carbon dioxide as an anti-solvent to selectively fractionate ethanolic and hydroalcoholic extracts of green propolis by precipitation in four separators in series.…”

Section: Resultsmentioning

confidence: 99%

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Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction

et al. 2018

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Propolis is a natural product with many demonstrated biological activities and propolis extract has been used in the food, pharmaceutical and cosmetics industries. Different works have showed the variations in the chemical composition, and consequently, on the biological activity of the propolis that are associated with its type and geographic origin. Due to this study evaluated propolis extracts obtained through supercritical extraction and ethanolic extraction (conventional) in three samples of different types of propolis (red, green and brown), collected from different regions in Brazil (state of Bahia). Analyses were performed to determine the humidity, water activity, the content of total ash, proteins, lipids and fiber in raw propolis samples. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH), catechin, ferulic acid and luteolin and antimicrobial activity against two bacteria (Staphylococcus aureus and Escherichia coli) were determined for all extracts. For the green and red ethanolic extracts the anti-leishmanicidal potential was also evaluated. The physicochemical profiles showed agreement in relation to the literature. The results identified significant differences among the extracts (p>0.05), which are in conformity with their extraction method, as well as with type and botanical origin of the samples. The extraction with supercritical fluid was not efficient to obtain extracts with the highest contents of antioxidants compounds, when compared with the ethanolic extracts. The best results were shown for the extracts obtained through the conventional extraction method (ethanolic) indicating a higher selectivity for the extraction of antioxidants compounds. The red variety showed the largest biological potential, which included the content of antioxidants compounds. The results found in this study confirm the influence of the type of the raw material on the composition and characteristics of the extracts. The parameters analysis were important to characterize and evaluate the quality of the different Brazilian propolis extracts based on the increased use of propolis by the natural products industry.

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Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction

et al. 2018

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“…According to the results in Table 4 and Fig 3, significant differences (p>0.05) were observed for the levels of formononetin and kaempferol when comparing the two extraction methods applied for the same sample, and when comparing the extracts obtained by the same method for samples of different origins. Thus, this study also proves that the extraction method [50,118–120] and geographical origin [121,122] influence the content of specific compounds in propolis extracts. The achieved results and statistical analysis have shown how ultrasound-assisted extraction resulted in shorter extraction time, and increased extraction capacity of biomarkers with high antioxidant activity from Brazilian red propolis.…”

Section: Resultssupporting

confidence: 69%

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

et al. 2019

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Propolis is a complex mixture of resinous and balsamic material collected from the exudates of plants, shoots, and leaves by bees. This study evaluated red propolis extracts obtained by conventional (ethanolic) extraction and ultrasound-assisted extraction of six samples from different regions of northeastern Brazil. The total phenolic compounds and flavonoids, in vitro antioxidant activity, concentration of formononetin and kaempferol and the cytotoxicity against four human tumor cell lines were determined for all twelve obtained extracts. Significant variations in the levels of the investigated compounds were identified in the red propolis extracts, confirming that the chemical composition varied according to the sampling region. The extraction method used also influenced the resulting propolis compounds. The highest concentration of the compounds of interest and the highest in vitro antioxidant activity were exhibited by the extracts obtained from samples from state of Alagoas. Formononetin and kaempferol were identified in all samples. The highest formononetin concentrations were identified in extracts obtained by ultrasound, thus indicating a greater selectivity for the extraction of this compound by this method. Regarding cytotoxic activity, for the HCT-116 line, all of the extracts showed an inhibition of greater than 90%, whereas for the HL-60 and PC3 lines, the minimum identified was 80%. In general, there was no significant difference (p>0.05) in the antiproliferative potential when comparing the extraction methods. The results showed that the composition of Brazilian red propolis varies significantly depending on the geographical origin and that the method used influences the resulting compounds that are present in propolis. However, regardless of the geographical origin and the extraction method used, all the red propolis samples studied presented great biological potential and high antioxidant activity. Furthermore, the ultrasound-assisted method can be efficiently applied to obtain extracts of red propolis more quickly and with high concentration of biomarkers of interest.

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Section: Introductionmentioning

confidence: 99%

“…The solvent accelerated extraction (SAE) method is a fast process that uses solvent under high pressure to promote greater desorption of the analytes present in the matrix, offering less exposure to organic solvents while producing more selective extracts [19]. Pressure liquid extraction (PLE) has been successfully used to obtain compounds of low polarity from propolis samples [19,20]. Although high pressurized ethanol (in supercritical state) has been previously applied to extract chemical compounds from Brazilian red propolis [21], the use of PLE with low-polarity solvents (such as hexane) to extract chemical compounds with potential anticancer activity has not been studied so far.…”

Section: Introductionmentioning

confidence: 99%

Brazilian Red Propolis: Extracts Production, Physicochemical Characterization, and Cytotoxicity Profile for Antitumor Activity

et al. 2020

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Brazilian red propolis has been proposed as a new source of compounds with cytotoxic activity. Red propolis is a resinous material of vegetal origin, synthesized from the bees of the Appis mellifera family, with recognized biological properties. To obtain actives of low polarity and high cytotoxic profile from red propolis, in this work, we proposed a new solvent accelerated extraction method. A complete 23 factorial design was carried out to evaluate the influence of the independent variables or factors (e.g., temperature, number of cycles, and extraction time) on the dependent variable or response (i.e., yield of production). The extracts were analyzed by gas chromatography coupled with mass spectrometry for the identification of chemical compounds. Gas chromatography analysis revealed the presence of hydrocarbons, alcohols, ketones, ethers, and terpenes, such as lupeol, lupenone, and lupeol acetate, in most of the obtained extracts. To evaluate the cytotoxicity profile of the obtained bioactives, the 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide colorimetric assay was performed in different tumor cell lines (HCT116 and PC3). The results show that the extract obtained from 70 °C and one cycle of extraction of 10 min exhibited the highest cytotoxic activity against the tested cell lines. The highest yield, however, did not indicate the highest cytotoxic activity, but the optimal extraction conditions were indeed dependent on the temperature (i.e., 70 °C).

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Fractionation of ethanolic and hydroalcoholic extracts of green propolis using supercritical carbon dioxide as an anti-solvent to obtain artepillin rich-extract

Cited by 15 publications

References 40 publications

Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction

Chemical characterization and biological activity of six different extracts of propolis through conventional methods and supercritical extraction

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

Brazilian Red Propolis: Extracts Production, Physicochemical Characterization, and Cytotoxicity Profile for Antitumor Activity

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