Fractionation of bovine whey proteins and characterisation by proteomic techniques

Proteomic analysis of whey from bovine colostrum and mature milk

“…2), but they did not interfere with the analysis of minor proteins (Golinelli, 2009). Furthermore, a good correlation was observed between the present data (Mr and pI) and the data from other studies (Yamada et al, 2002;Fong et al, 2008), indicating that no degradation and/or artifacts were introduced during the removal method and 2D-PAGE. The bovine colostrum and mature milk whey contain salts and other substances that also can interfere in the resolution of protein spots in 2D electrophoresis.…”

“…Bovine whey in particular contains more than 200 different proteins, with β-lactoglobulin, α-lactalbumin, bovine serum albumin (BSA), and immunoglobulins as the major constituents (Sgarbieri, 2004). Knowledge of the identity and function of the minor protein constituents of bovine milk whey is still incomplete, although many of these proteins may have biological properties that contribute to the health benefits of whey (Fong et al, 2008;Rusu et al, 2009). Bulk bovine whey has been used in the production of nutraceuticals (Rusu et al, 2009) and as raw material for several other products (Revillion et al, 2003;Mariotti et al, 2008).…”

“…Approaches such as immunoabsorption (Murakami et al, 1998;Palmer et al, 2006;Yamada et al, 2002), solution isoelectric focusing (IEF) (Zuo and Speicher, 2002), affinity tagging (Holland et al, 2006;Conte-Junior et al, 2006), and semi-coupled anion-and cation-exchange chromatography (Fong et al, 2008) have been used to remove the dominant proteins and increase the relative abundance of the minor proteins, to aid proteomic analysis of complex samples such as bovine milk. Proteomic methodology has been used in the study and characterization of proteins from milk and dairy products (Yamada et al, 2002;Manso et al, 2005;Fong et al, 2008). However, the large amount of immunoglobulins in bovine whey is particularly troublesome for the proteomic analysis of the minor proteins (Yamada et al, 2002).…”

“…However, the large amount of immunoglobulins in bovine whey is particularly troublesome for the proteomic analysis of the minor proteins (Yamada et al, 2002). Immunoglobulins are located in a region of the 2D-gel where most of the minor proteins, such as lactoferrin, transferrin, plasmin, lipoprotein lipase, alkaline phosphatase, and others yet to be identified are found (Fong et al, 2008). Several methods can be used to identify new proteins in complex samples.…”

Comparison of the principal proteins in bovine, caprine, buffalo, equine and camel milk

“…Proteomic techniques are ideally suited to obtaining such information and have been applied to the study of bovine milk and milk products (Holt & Zeece, 1987;Chin & Rosenberg, 1998;Holland et al 2004;Lindmark-Månsson et al 2004;Fong et al 2008;Pappa et al 2008). However, only a few studies have examined non-bovine milks using the proteomic approach.…”