Fractionation Of Bovine Lutropin With The Aid Of Anion-Exchange High Performance Liquid ChromatographY

Journal of Liquid ChromatographyPublication details, including instructions for authors and subscription information: ABSTRACTThe Pharmacia Fast Protein Liquid Chromatography System equiped w i t h characterize the distribution of different bioactive forms of a cynomol gus ( Macaca ~a n c i c u &~~i~ ) pituitary 1 utropi n preparation. The results were compared w i t h the profiles obtained a f t e r running the preparation on a Waters DEAE anion exchange column usin eluants in the pH range from 6.0 t o 8.0..The distribution 09 preserved biologica! activity were studied i n respective eluted.fractions from the different experiments of the appl ied preparation. Seventy-six ercent of the bioactivity was recovered a f t e r chromamatography ran ed between 61-78 % a t pH 6.5 to 7.5. No bioactivity was restored a f t e r elution a t pH 6.0 or 8.0. Present adress : * KabiVitm AB Peptide Hormones, Mono P HR 5(10 chromatofocusing column.was used t o tofocusing w R ereas preserved bioactivity a f t e r ion exchayge chro-S-112 87 Stockholrn Sweden 1261

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Fractionation Of Bovine Lutropin With The Aid Of Anion-Exchange High Performance Liquid ChromatographY

Cited by 4 publications

References 11 publications

Evaluation of an immunoradiometric assay for thyrotropin in serum and plasma samples of dogs with primary hypothyroidism

Evaluation of an immunoradiometric assay for thyrotropin in serum and plasma samples of dogs with primary hypothyroidism

Separable Forms of Radioiodinated Ovine Lutropin (oLH) Subunits, Fractionated by Anion Exchange High Performance Liquid Chromatography and Analyzed by Radioimmunoassay

Biological Characterization of a Pituitary Monkey Lutropin Preparation after Chromatofocusing or after High Performance Anion Exchange Chromatography

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