Evaluation of an immunoradiometric assay for thyrotropin in serum and plasma samples of dogs with primary hypothyroidism

Jensen, A. L.; Iversen, Lars; Høier, R.; Kristensen, F.; Henriksens, P.

doi:10.1016/s0021-9975(96)80055-7

Cited by 13 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two other studies have evaluated cTSH measurements for the diagnosis of hypothyroidism (Jensen andothers 1996, Ramsey andothers 1997). Comparisons were made to thyroid biopsy (Jensen and others 1996) or TRH response test results (Ramsey and others 1997).…”

Section: Dlscusslonmentioning

confidence: 99%

Evaluation of serum free thyroxine and thyrotropin concentrations in the diagnosis of canine hypothyroidism

Dixon

Mooney

1999

J of Small Animal Practice

View full text Add to dashboard Cite

Canine thyroihtimulating hormone (cTSH), total thyroxine (T4) and free T4 by equilibrium dialysis (fT4d) were measured in serum samples from 107 dogs with clinical signs suggestive of hypothyroidism in which the diagnosis was either confirmed (n=30) or excluded (n=77) by exogenous TSH response testing. Median serum total T4 and fT4d concentrations were significantly lower and cTSH significantly higher (P

show abstract

Section: Dlscusslonmentioning

confidence: 99%

Evaluation of serum free thyroxine and thyrotropin concentrations in the diagnosis of canine hypothyroidism

Dixon

Mooney

1999

J of Small Animal Practice

View full text Add to dashboard Cite

show abstract

“…[2][3][4] However, in as many as a third of dogs with primary hypothyroidism, plasma TSH concentrations are within the reference range. [2][3][4]13,14 Thus, in dogs, the differentiation between hypothyroidism and nonthyroidal illness has become a thorny problem, because of both the common occurrence of low plasma TT 4 concentrations in nonthyroidal illness and the frequent absence of a high plasma TSH concentration in primary hypothyroidism. Therefore, in dogs, histologic examination of thyroid tissue obtained by biopsy is regarded as the definitive and the most sensitive test to identify thyroid disease.…”

mentioning

confidence: 99%

Assessment of Thyroid Function in Dogs with Low Plasma Thyroxine Concentration

Espiñeira¹,

Mol²,

Peeters³

et al. 2007

Veterinary Internal Medicne

Self Cite

View full text Add to dashboard Cite

Background: Differentiation between hypothyroidism and nonthyroidal illness in dogs poses specific problems, because plasma total thyroxine (TT 4 ) concentrations are often low in nonthyroidal illness, and plasma thyroid stimulating hormone (TSH) concentrations are frequently not high in primary hypothyroidism.Hypothesis: The serum concentrations of the common basal biochemical variables (TT 4 , freeT 4 [fT 4 ], and TSH) overlap between dogs with hypothyroidism and dogs with nonthyroidal illness, but, with stimulation tests and quantitative measurement of thyroidal 99m TcO 4 2 uptake, differentiation will be possible. Animals: In 30 dogs with low plasma TT 4 concentration, the final diagnosis was based upon histopathologic examination of thyroid tissue obtained by biopsy. Fourteen dogs had primary hypothyroidism, and 13 dogs had nonthyroidal illness. Two dogs had secondary hypothyroidism, and 1 dog had metastatic thyroid cancer.Methods: The diagnostic value was assessed for (1) plasma concentrations of TT 4 , fT 4 , and TSH; (2) TSH-stimulation test; (3) plasma TSH concentration after stimulation with TSH-releasing hormone (TRH); (4) occurrence of thyroglobulin antibodies (TgAbs); and (5) thyroidal

show abstract

“…Ramsey (1997) reported a reference range for cTSH concentrations between 0 and 0.41 ng/ml. Jensen et al (1996) reported even lower reference range values in healthy dogs (0.06 -0.34 ng/ml).…”

Section: Discussionmentioning

confidence: 87%

“…However, some studies suggest that up to one quarter of the dogs with primary hypothyroidism show cTSH concentrations within the reference range (Jensen et al 1996;Peterson et al 1997;Ramsey et al 1997;Scott-Moncrieff et al 1998;Dixon and Mooney 1999;Kooistra et al 2000). So far no studies have been published under local conditions evaluating the diagnostic value of this approach.…”

mentioning

confidence: 99%

Parallel Determination of Total Thyroxine and Thyrotropin Concentrations in Diagnosis of Primary Hypothyroidism in the Dog

Kolevská¹,

Svoboda²,

Brunclík³

2002

Acta Vet. Brno

View full text Add to dashboard Cite

The objective of the work was to verify the validity of parallel determination of total thyroxine (tT 4 ) and thyrotropin (cTSH) concentrations in the diagnosis of primary hypothyroidism in the dog. The concentrations of total thyroxine and thyrotropin were determined by chemiluminiscence immunoassay in the serum of a total of 117 dogs: 40 negative controls, 66 dogs with euthyroid sick syndrome and 11 patients with primary hypothyroidism. The average tT 4 concentration in the group of healthy dogs was 25.8 ± 5.48 nmol/l and the average cTSH concentration was 0.17 ± 0.13 ng/ml. In the group of patients with primary hypothyroidism the tT 4 concentrations were significantly decreased in 10 out of 11 cases (10.12 ± 2.01 nmol/l), while the cTSH concentrations were significantly increased (2.9 ± 2.04 ng/ml). In one patient with primary hypothyroidism, only the tT 4 concentration was decreased to 9.0 nmol/l, but the cTSH concentration was found to be within the reference range (0.04 ng/ml). The average tT 4 concentration in the group of patients with euthyroid sick syndrome was 11.7 ± 3.16 nmol/l, while the average cTSH concentration in the same group of patients was 0.14 ± 0.12 ng/ml. The concentrations of the two hormones mentioned above were compared between the different groups using the non-parametric Mann-Whitney test. The concentration of tT 4 was significantly higher (p < 0.01) in the group of healthy dogs compared to the dogs with primary hypothyroidism and the dogs with euthyroid sick syndrome. The concentration of cTSH was significantly higher (p < 0.01) in the dogs with primary hypothyroidism compared to the healthy dogs and the dogs with euthyroid sick syndrome. The results of this work show that parallel determination of tT 4 and cTSH represents a highly sensitive and specific method which is suitable for differentiating between primary hypothyroidism and other diseases that significantly reduce tT 4 concentrations.Canine TSH, tT 4 , euthyroid sick syndrome

show abstract

Evaluation of an immunoradiometric assay for thyrotropin in serum and plasma samples of dogs with primary hypothyroidism

Cited by 13 publications

References 12 publications

Evaluation of serum free thyroxine and thyrotropin concentrations in the diagnosis of canine hypothyroidism

Evaluation of serum free thyroxine and thyrotropin concentrations in the diagnosis of canine hypothyroidism

Assessment of Thyroid Function in Dogs with Low Plasma Thyroxine Concentration

Parallel Determination of Total Thyroxine and Thyrotropin Concentrations in Diagnosis of Primary Hypothyroidism in the Dog

Contact Info

Product

Resources

About