Fractionation and purification of membrane lipids from the archaeon Thermoplasma acidophilum DSM 1728/10217

Molecular species determination of oligosaccharides and glycoconjugates in soybean lecithin powders

“…Every 100 mL eluent was collected to evaporate under reduced pressure; TLC developed in chloroform/methanol (90:10, v/v) was used to distinguish the different glycoconjugates fractions, and fractions sharing the same Rf value were combined and identified as one compound. The color reagent of TLC was 50% (w/w) sulfuric acid, after the silica gel plate is sprayed with color agent, it needs to be colored at 135 °C for 10 minutes . For preliminary qualitative glycoconjugates, the Molish reaction and Liebermann–Burchard reaction were applied to test for the existence of sugar and sterol in the isolated glycoconjugate fractions.…”

“…The color wileyonlinelibrary.com/jsfa reagent of TLC was 50% (w/w) sulfuric acid, after the silica gel plate is sprayed with color agent, it needs to be colored at 135 ∘ C for 10 minutes. 28 For preliminary qualitative glycoconjugates, the Molish reaction 29 and Liebermann-Burchard reaction were applied to test for the existence of sugar and sterol in the isolated glycoconjugate fractions.…”

Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells

“…Lipid purification, in general, is very time-consuming and cost-intensive. 6 Hence, for pharmaceutical application in oral drug delivery or oral vaccination, the use of sufficiently purified membrane fractions may be advantageous over highly purified single lipids. Purified membrane fractions both from Thermoplasma and Sulfolobus species form stable archaeosomes.…”

Characterization of Thermoplasma Species Cultured from Sampling on Tangkuban Perahu, Indonesia

“…1994) was chromatographed under the same condition. Apart from the total lipid extract of strain DSM 1728, we had a fraction with mild hydrolization of the phoshoester of MPL (Antonopoulos . 2013).…”