Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells

Oertl, Anton; Antonopoulos, E; Freisleben, Seruni U.; Freisleben, Hans-Joachim

doi:10.13181/mji.v25i4.1506

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Although CF leakage was much lower from MPL liposomes than from those made of egg lecithin or DPPC [70], hydrophilic CF was transferred very well from MPL liposomes to trypsinated T84 tumor cells and rapid intermembrane exchange of lipophilic bromobimane and Nile red from MPL liposomal membrane into isolated red cell membrane (ghosts) was demonstrated [71].…”

Section: Model Membranesmentioning

confidence: 99%

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The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

Freisleben

2019

IJMS

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The main phospholipid (MPL) of Thermoplasma acidophilum DSM 1728 was isolated, purified and physico-chemically characterized by differential scanning calorimetry (DSC)/differential thermal analysis (DTA) for its thermotropic behavior, alone and in mixtures with other lipids, cholesterol, hydrophobic peptides and pore-forming ionophores. Model membranes from MPL were investigated; black lipid membrane, Langmuir-Blodgett monolayer, and liposomes. Laboratory results were compared to computer simulation. MPL forms stable and resistant liposomes with highly proton-impermeable membrane and mixes at certain degree with common bilayer-forming lipids. Monomeric bacteriorhodopsin and ATP synthase from Micrococcus luteus were co-reconstituted and light-driven ATP synthesis measured. This review reports about almost four decades of research on Thermoplasma membrane and its MPL as well as transfer of this research to Thermoplasma species recently isolated from Indonesian volcanoes.

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Section: Model Membranesmentioning

confidence: 99%

“…A wide range of biomedical and biotechnological applications of TEL and liposomes of TEL has been presented earlier [7,55,71] including oral delivery of vaccines and acid-sensitive pharmaceuticals [56,81]. Chemical modification resulting in cationic tetraether lipids for transfections was reported [82].…”

Section: Applicationsmentioning

confidence: 99%

The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

Freisleben

2019

IJMS

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How Can Immuno suppression After Organ Transplantation be Made More Effective and Safer? – A Review on Liposomal Formulations with Consideration of Archaeal Tetraetherlipid

Purwaningsih

Oertl

Freisleben

et al. 2021

Biomed. Pharmacol. J.

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Immune-suppressive agents such as methylprednisolone and cyclosporine exert tremendous side effects, because of high dosage and long-term application required for immune suppression after organ transplantation. Major side effects of methylprednisolone include bleeding of the gastro-intestinal tract, hypertension, and osteoporosis, whereas cyclosporine is nephrotoxic. Liposomes are phospholipid particles that allow delivery of drugs preferentially to the reticuloendothelial system. They can be prepared from phospholipids such as lecithin from soybean or egg yolk, other specific or modified lipids or from membrane-spanning tetraether lipid (TEL), which can be extracted and purified from archaeal cell membranes. One advantage in the use of liposomal application is reduced toxicity of many drugs. We report on various liposomal preparations of cyclosporine, methylprednisolone (L-MPL) and its palmitate derivative (L-MPLP). It has been documented that liposomal cyclosporine A (L-CsA), 1.75 mg/kg/day for seven days has potential for use as an immune-suppressive agent in rats with increased efficacy and decreased nephrotoxicity compared to commercially available forms of intravenous CsA. Liposomal methylprednisolone (L-MPL) 2 mg/kg, intravenously (IV), twice a week shows significantly prolonged cardiac allograft survival in rats and tissue-selective sequestration of the drug in comparison with the same dosage regimen of methylprednisolone in solution, administered daily. We report on organ distribution of L-MPLP in rats after intraperitoneal (IP) administration. Conclusion: Liposomal preparations of immunosuppressants have significantly higher immune-suppressive potential and lower toxicity than non-liposomal preparations. Bipolar TEL can be extracted, fractionated and purified from archaea to form stable liposomes which are extremely resistant, even to gastric fluid. Hence, TEL liposomes allow (besides IV and IP) for oral administration of immuno suppressants after organ transplantation with pharmacological and toxicological advantages as common liposomal phospholipid bilayer preparations.

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Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells

Abstract: ABSTRAK

Cited by 2 publications

References 21 publications

The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

How Can Immuno suppression After Organ Transplantation be Made More Effective and Safer? – A Review on Liposomal Formulations with Consideration of Archaeal Tetraetherlipid

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