Fractionation and Properties of Trypsin and Chymotrypsin Inhibitors from Lima Beans

Derivative spectroscopy applied to tyrosyl chromophores. Ribonuclease, lima bean inhibitors, insulin, and pancreatic trypsin inhibitor

“…Previous studies have shown four (Jones et al, 1963) or five (Ikeda et al, 1968) well-resolved components on DEAEcellulose and six components on DEAE-Sephadex (Haynes and Feeney, 1967). In all cases, the second to last component off of the column was the only component shown by amino acid analysis to contain more than one tyrosine per mole;…”

Purification and properties of soybean protein inhibitors of proteolytic enzymes

“…(Yamamoto and Ikenaka, 1967), raises the distinct possibility that the two are the same. However, considering that families of closely related inhibitors have been shown to exist (Haynes and Feeney, 1967;Jones et al, 1963), the status of the relationship between the two will depend on further study. Physical Characteristics.…”

“…Nevertheless, the distinction of being the smallest known proteinase inhibitor belongs to the 3000 to 4000 molecular weight carboxypeptidase B inhibitor isolated from potatoes (Rancour and Ryan, 1968). In any event, the low value for the soybean inhibitor puts it in a size class comparable to a family of lima bean inhibitors (Haynes and Feeney, 1967;Jones et al, 1963), inhibitors from kidney bean (Pusztai, 1968) and Phciseolus aureus (Chu et al, 1964), and, interestingly, two pancreatic proteinase inhibitors (Greene et al, 1966;Kassell et al, 1965). From the standpoint of genetic relationships or taxonomic classification, investigation of structural homologies of the plant inhibitors could prove of some value.…”

Trypsin inhibitor from Lathyrus sativus seeds: final purification, separation of protein components, properties, and characterization

“…Extensive work has been done in various components of trypsin inhibitors from soybean which has been reviewed (Steiner and Frattali, 1969;Kassell, 1970), and it has been observed that soybean seems to have at least five and possibly six protease inhibitors (Liener and Kakade, 1969). Similarly, lima bean has six components (Fraenkel-Conrat et al, 1952;Tauber et al, 1949;Jones et al, 1963 and Feeney, 1967), and navy bean has five protein components with antitryptic properties (Bowman, 1971). If a comparison is made in the light of physicochemical properties of the LSTI-B" along with its individual protein components with some of the reported values of the plant protease inhibitors (Liener and Kakade, 1969), it seems that all of them are different in their own way; but still, if an attempt is made to draw some type of similarities, the properties like isoelectric point, extinction coefficient, molecular weight, stoichiometry, and amino acid composition may be comparable between LSTI-B" and its components, with Kunitz inhibitors, Bowman-Birk inhibitors, 1.9S inhibitors from soybean, and also with those of navy beans.…”