Fractionation and characterization of antioxidant peptides derived from barley glutelin by enzymatic hydrolysis

Effect of Enzyme Type on the Antioxidant Activities and Functional Properties of Enzymatic Hydrolysates from Sea Cucumber (Cucumaria frondosa) Viscera

“…Besides, it could be found that DPPH radical scavenging rate was lower than hydroxyl radical scavenging rate for the same hydrolysate at the same concentration, which should be correlated with the ratio of hydrophilic and hydrophobic amino acids. The reducing power assay is often used to evaluate the ability of natural antioxidant to donate an electron or hydrogen (Xia et al, 2012). As described in Figure 4(C), alcalase, trypsin and flavourzyme gave a much higher reducing power to the hydrolysates than the other four proteases (p < 0.05), revealing that the three hydrolysates have more active amino acids or peptides to react with free radicals to form more stable products.…”

“…It can react with biomolecules such as amino acids, proteins, and DNA, as well as trigger lipid peroxidation (Xie et al, 2008). Therefore, removal of the hydroxyl radical is probably one of the most effective defenses of a system against oxidation (Xia et al, 2012). Previous study showed that protein hydrolysates may scavenge hydroxyl radical by electron/hydrogen donation and radical quenching (Kong and Xiong, 2006).…”

“…As shown in Figure 4(A), at the same concentration (2 mg/mL), trypsin conferred the strongest ability to scavenge hydroxyl radical on the hydrolysate (p < 0.05), the scavenging rate reaching 27.04%; and neutrase, alcalase, and flavourzyme were the second efficient enzyme, the rate being 23.86, 23.76, and 22.96%, respectively. DPPH is a stable free radical and accepts an electron of hydrogen radical to become a stable diamagnetic molecule (Xia et al, 2012). The model of scavenging this radical has been widely applied to evaluate the free radical scavenging activity of various antioxidants (Zhao et al, 2012;Klompong et al, 2007;Farvin et al, 2014).…”

Antihypertensive Properties of Plant Protein Derived Peptides

“…They reported that using different enzymes resulted in the formation of a mixture of peptides with different degrees of hydrolysis and accordingly different ranges of antioxidant activity. It has been found that antioxidant activity of Alcalase derived hydrolysates is higher than that of other hydrolysates [52][53][54]. It is also reported that peptides produced by Alcalase have diverse biological activities, including antioxidant activity [55].…”

“…Large size peptides possessed stronger DPPH scavenging activity and reducing power, whereas small-sized peptides were more effective in Fe 2+ and hydroxyl radical scavenging activity [54]. Pepsin hydrolysis of byproduct of the wheat starch industry has shown antioxidant properties.…”