Fractionation and antioxidant activity potency of the extract of Garcinia lateriflora Blume var. javanica Boerl leaf

Abstract

Preparation and characterization of cross-linked excipient of coprocessed xanthan gumacacia gum as matrix for sustained release tablets AIP Conference Proceedings 1933, 030009 (2018 Abstract. Garcinia lateriflora leaves extract of the family Guttiferae has been known to have excellent antioxidant activity. The objective of the study was to determine the antioxidant effect of the n-hexane, ethyl acetate and methanol extracts of G. lateriflora leaves extract. The antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging methods and Feric Reducing Antioxidant Power (FRAP) to determine the antioxidant properties. The extracts were fractionated by using column chromatography. The Methanol extract exhibited the strongest antioxidant activity with EC50 values are 13.95 and 19.65 μg/mL by DPPH and FRAP methods respectively. E13 fraction was the most active fraction from ethyl acetate extract with EC50 value for DPPH scavenging method was 37.14 μg/mL and 34.46 μg/mL for reducing power by the FRAP method. Meanwhile M3 fraction was the most active fraction in methanol extract with EC50 value for DPPH scavenging method was 50.02 μg/mL and 37.32 μg/mL for reducing power by the FRAP method.

Phytochemical Analysis, HPLC Profiling and Anti-oxidant Potential of Euphorbia nivulia Buch.-Ham

“…Our findings showed that polar fractions, in current study, exhibited highest phenolic and flavonoid content. These results are in accordance with previous data that showed that phenolic compounds being polar in nature are found in polar fractions [34]. In current study, ENBF exhibited highest phenolic content (143.26±2.65 mg/g GA) followed by ENCr (125.6±1.32 mg/g GA), ENAF (96.53±2.01 mg/g GA), ENCF (38.27±3.21 mg/g GA) and ENHF (15.49±1.92 mg/g GA) as shown in Fig.…”

Phytochemical Contents and Antioxidant Activities of Etlingera elatior Leaf Extract and Fractions

“…This could be due to a compound found in ethanol extract that, while being in a solvent with different polarity, tends to work in synergy. 33 The antioxidant activity test on the 96% ethanol extract, water, ethyl acetate, and n-hexane fractions inhibited the DPPH radicals with IC50 values of 44.86, 45.31, 40.48, and 39.35 µg/mL, respectively compared to the Trolox 3.49 µg/mL (Table 2). Based on this observation, the less non-polar the solvent used, the lower the IC50 value.…”

“…Because the ABTS technique is light-sensitive, even the synthesis of ABTS•requires a 12-to 16-hour incubation period in the dark. 33 The antioxidant testing with the ABTS method is proportionate to DPPH, specifically the nhexane fraction, which has a very strong antioxidant activity (< 50 µg/mL). Both the DPPH and ABTS methods for determining the antioxidant are in vitro procedures.…”

Effect of Extraction Methods on the Antioxidants and Alpha-Glucosidase Inhibitory Activity of Borassus flabellifer L. Fruit Fiber

“…A blank mixture of DPPH and ethanol was used as a control. The percentage of DPPH radical scavenging was measured using the following equation: 23,25…”