Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES

Rebocho, Sílvia; Mano, Francisca; Cassel, Eduardo; Anacleto, Beatriz; Bronze, Maria do Rosário; Paiva, Alexandre; Duarte, Ana Rita C.

doi:10.1016/j.crfs.2022.03.004

Cited by 20 publications

(2 citation statements)

References 51 publications

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“…Methanol, acetone (70%), ethanol and ethyl acetate are the most commonly used solvents for polyphenols [ 4 , 18 ]. As an alternative to toxic organic solvents, a new class of green solvents, natural deep eutectic solvents (NADES), have been applied in sample preparation and analytical techniques [ 35 ] for the efficient extraction and separation of a wide range of natural compounds [ 36 ] including polyphenols [ 37 , 38 , 39 ]. UAE was used to increase the efficiency of NADES in the extraction of phenolic compounds [ 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry

et al. 2023

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Phlorotannins are secondary metabolites produced mainly by brown seaweeds (Phaeophyceae) and belong to the class of polyphenolic compounds with diverse bioactivities. The key factors in the extraction of polyphenols are the selection of a suitable solvent, method of extraction and selection of optimal conditions. Ultrasonic-assisted extraction (UAE) is one of the advanced energy-saving methods suitable for the extraction of labile compounds. Methanol, acetone, ethanol and ethyl acetate are the most commonly used solvents for polyphenol extraction. As alternatives to toxic organic solvents, a new class of green solvents, natural deep eutectic solvents (NADES), has been proposed for the efficient extraction of a wide range of natural compounds including polyphenols. Several NADES were screened previously for the extraction of phlorotannins; however, the extraction conditions were not optimized and chemical profiling of NADES extract was not performed. The purpose of this work was to study the effect of selected extraction parameters on the phlorotannin content in NADES extract from Fucus vesiculosus, optimization of extraction conditions and chemical profiling of phlorotannins in the NADES extract. A fast and green NADES-UAE procedure was developed for the extraction of phlorotannins. Optimization was performed through an experimental design and showed that NADES (lactic acid:choline chloride; 3:1) provides a high yield (137.3 mg phloroglucinol equivalents per g dry weight of algae) of phlorotannins under the following extraction conditions: extraction time 23 min, 30.0% water concentration and 1:12 sample to solvent ratio. The antioxidant activity of the optimized NADES extract was equal to that of EtOH extract. In total, 32 phlorotannins have been identified (one trimer, two tetramers, six pentamers, four hexamers, six heptamers, six octamers and seven nonamers) in NADES extracts from arctic F. vesiculosus using the HPLC-HRMS and MS/MS technique. It was noted that all the above-mentioned phlorotannins were identified in both EtOH and NADES extracts. Our results suggest that NADES could be considered as an alternative to the conventional techniques for the effective extraction of phlorotannins from F. vesiculosus with high antioxidant potential.

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Section: Introductionmentioning

confidence: 99%

Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry

et al. 2023

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“…Additionally, Dai et al [ 41 ] reported that LA:Glu systems provide better stability of cyanidin when compared to ethanol extractions. These results can be explained due to the interaction between the phenolic compounds and the components of NADES mixtures, which decrease the movement of solutes molecules and reduce their contact time with oxygen, and in consequence, the oxidative degradation is reduced [ 41 , 42 ]. The enhanced stability of TPC in NADES extracts indicates their potential application in the fortification of food products, and this characteristic can contribute to extending the shelf life of the enriched products.…”

Section: Resultsmentioning

confidence: 99%

Sustainable Development and Storage Stability of Orange By-Products Extract Using Natural Deep Eutectic Solvents

Gómez-Urios

Viñas-Ospino

Puchades-Colera

et al. 2022

Foods

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The citrus industry produces large amounts of waste rich in bioactive compounds that have important effects on human health. Their extraction was performed using organic solvents, and a greener alternative to those solvents are natural deep eutectic solvents (NADES). The present study aimed to obtain and optimize extracts rich in polyphenols and flavonoids from orange peels using NADES and monitor polyphenol stability in the extracts for 30 days. The software COSMOtherm (conductor-like screening model) was used to screen fourteen NADES. The most promising solvents were lactic acid:glucose (LA:Glu) with an extraction yield of 1932 ± 7.83 mgGAE/100 gdw for TPC (total polyphenol content) and 82.7 ± 3.0 mg/100 gdw for TFC (total flavonoid content) and in the case of L-proline:malic acid (LP:MA) was 2164 ± 5.17 mgGAE/100 gdw for TPC and 97.0 ± 1.65 mg/100 gdw for TFC. The extraction process using LA:Glu and LP:MA was optimized, and the results showed that the selected variables (%NADES, solid:liquid ratio, and extraction time) had a significant influence on the extraction of TPC and TFC. Results showed that NADES improve the stability of TPC. These findings revealed that NADES are efficient for the extraction of bioactive compounds from orange by-products, and these extracts can represent an alternative for the food industry to enrich food products with natural ingredients.

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Improving carotenoid extraction, stability, and antioxidant activity from Citrus sinensis peels using green solvents

Viñas-Ospino

López-Malo

Esteve

et al. 2023

Eur Food Res Technol

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Carotenoids have a wide range of applications in the food, pharmaceutical, and cosmetic industries as natural coloring agents and antioxidants. Consequently, industries are more concerned about extracting them from natural resources and by-products. The present research aimed to evaluate the extraction efficiency of carotenoids from orange peels using hydrophobic deep eutectic solvents (HDESs) as alternatives for organic solvents. The antioxidant capacity and color stability of HDESs extracts were monitored for 20 days and to intensify the extraction process, ultrasound-assisted extraction (UAE) was optimized using a response surface methodology (RSM). Menthol:Eucalyptol (Me:Eu) extract showed the highest carotenoid extraction yield [359.3 ± 3.5 mg/100 g of fresh weight (fw)], and also presented high stability during the storage period. HDESs extracts showed higher antioxidant capacity compared to hexane extracts, while Me:Eu extracts showed the lowest color variation (5.9 ± 0.2). Optimal parameters using Me:Eu were extraction time of 20 min, ultrasonic power of 120 W (60%), and solid–liquid ratio of 1:20 (g/mL) reaching a carotenoid content of 573.4 mg/100 gfw. While, C12:C8 optimal parameters were 10 min, 80 W (40%), and a solid–liquid ratio of 1:10 (g/mL), providing a carotenoid content of 183.7 mg/100 gfw. To establish if the solvents used are greener alternatives, the EcoScale was used and showed that UAE is a sustainable method to recover carotenoids using HDESs. Overall, the results showed that HDESs can improve carotenoid stability, and when combined with the intrinsic safety and edibility of their components, it makes these extracts appealing for food industry.

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Fractionated extraction of polyphenols from mate tea leaves using a combination of hydrophobic/ hydrophilic NADES

Cited by 20 publications

References 51 publications

Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry

Optimization of Extraction of Phlorotannins from the Arctic Fucus vesiculosus Using Natural Deep Eutectic Solvents and Their HPLC Profiling with Tandem High-Resolution Mass Spectrometry

Sustainable Development and Storage Stability of Orange By-Products Extract Using Natural Deep Eutectic Solvents

Improving carotenoid extraction, stability, and antioxidant activity from Citrus sinensis peels using green solvents

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