Fractal Analysis of AFM Images of the Surface of Bowman’s Membrane of the Human Cornea

Ţălu, Ștefan; Stach, Sebastian; Sueiras, Vivian; Ziebarth, Noël Marysa

doi:10.1007/s10439-014-1140-3

Cited by 30 publications

(9 citation statements)

References 34 publications

(38 reference statements)

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“…In view of this, the use of AFM image processing to obtain stereometric parameters of surfaces has been widely studied in recent years (Stach et al, 2019;Sobola et al, 2020;Zaneta et al, 2017;Ţ alu et al, 2015). These measurements provide mechanisms for a better understanding of relevant aspects of a surface, like for example, roughness, peak distribution, hill regions, pit regions, and power spectrum density (PSD), among others.…”

Section: Morphology Analysismentioning

confidence: 99%

Stereometric characterization of kefir microbial films associated with Maytenus rigida extract

Matos

Pinto

Ramos

et al. 2020

Microscopy Res & Technique

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The aim of this study was to report the first discussion of statistical parameters and possible applications related to the surface of kefir biofilms prepared with Maytenus rigida Mart. extract through micromorphology, using atomic force microscopy (AFM). Four different samples were produced. The reference contained demerara sugar with a concentration of 40 g/L and the others had added extract whose concentrations were 0.25, 0.50, and 0.75 g/L, respectively. AFM was used to image the surface in tapping mode, on square areas of 30 μm × 30 μm. Images were studied by the MountainsMap premium commercial software. The main parameter of the surface, roughness, grew with the increase of the extract concentration up to 0.50 g/L. In addition, asymmetry and kurtosis showed that the biofilm with the best peak distribution was the one with 0.25 g/L of plant extract. The other parameters followed the main results for the surface and were observed for the depth and volume parameters. The power spectrum density spectrum confirmed the reliability of the results for the sample with 0.25 g/L. Therefore, the sample with 0.25 g/L of plant extract produced the best balance in relation to the surface properties. These results showed that the biofilm morphology and microtexture were affected by the incorporation of the plant extract and can be useful to define possible applications.

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Section: Morphology Analysismentioning

confidence: 99%

Stereometric characterization of kefir microbial films associated with Maytenus rigida extract

Matos

Pinto

Ramos

et al. 2020

Microscopy Res & Technique

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show abstract

“…It is important to note that there are many studies about thin films that have tried to understand 3D complex surface morphology through atomic force microscopy (AFM) measurements (Elenkova et al, 2015;Méndez et al, 2015;Sobola et al, 2017) using stereometric analysis (Ţălu et al, 2015a;Stach et al, 2015Stach et al, , 2017, fractal and multifractal geometry (Arman et al, 2015;Ţălu et al, 2014, 2015b, 2016a, 2019, and power spectral density functions (Arman et al, 2015;Gong et al, 2016). However, articles that use such analyses on other types of surfaces, such as plant leaves, are rare.…”

Section: Introductionmentioning

confidence: 99%

Advanced Microtexture Study of Anacardium occidentale L. Leaf Surface From the Amazon by Fractal Theory

2020

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This work applies stereometric parameters and fractal theory to characterize the structural complexity of the 3D surface roughness of Anacardium occidentale L. leaf using atomic force microscopy (AFM) measurements. Surface roughness was studied by AFM in tapping mode, in air, on square areas of 6,400 and 10,000 μm2. The stereometric analyses using MountainsMap Premium and WSXM software provided detailed information on the 3D surface topography of the samples. These data showed that the morphology of the abaxial and adaxial side of the cashew leaf is different, which was also observed in relation to their microtextures. Fractal analysis showed that the adaxial and abaxial sides have strong microtexture homogeneity, but the adaxial side presented higher surface entropy. These results show that image processing associated with fractal theory can be an indispensable tool for identifying plant species by their leaves because this species has singularities on each side of the leaf.

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“…For instance, the Bowman’s membrane of the human cornea is ascribed to display a fractal surface [16]. Furthermore, the suggestion of clot fractality as a biomarker for different stages of lung cancer adds further credence to this notion [17].…”

Section: Introductionmentioning

confidence: 99%

An Abraded Surface of Doxorubicin-Loaded Surfactant-Containing Drug Delivery Systems Effectively Reduces the Survival of Carcinoma Cells

et al. 2016

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An effective antitumor remedy is yet to be developed. All previous approaches for a targeted delivery of anticancer medicine have relied on trial and error. The goal of this study was to use structural insights gained from the study of delivery systems and malignant cells to provide for a systematic approach to the development of next-generation drugs. We used doxorubicin (Dox) liposomal formulations. We assayed for cytotoxicity via the electrical current exclusion method. Dialysis of the samples yielded information about their drug release profiles. Information about the surface of the delivery systems was obtained through synchrotron small-angle X-ray scattering (SAXS) measurements. SAXS measurements revealed that Dox-loading yielded an abraded surface of our Dox liposomal formulation containing soybean oil, which also correlated with an effective reduction of the survival of carcinoma cells. Furthermore, a dialysis assay revealed that a higher burst of Dox was released from soybean oil-containing preparations within the first five hours. We conclude from our results that an abraded surface of Dox-loaded drug delivery system increases their efficacy. The apparent match between surface geometry of drug delivery systems and target cells is suggested as a steppingstone for refined development of drug delivery systems. This is the first study to provide a systematic approach to developing next-generation drug carrier systems using structural insights to guide the development of next-generation drug delivery systems with increased efficacy and reduced side effects.

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Fractal Analysis of AFM Images of the Surface of Bowman’s Membrane of the Human Cornea

Cited by 30 publications

References 34 publications

Stereometric characterization of kefir microbial films associated with Maytenus rigida extract

Stereometric characterization of kefir microbial films associated with Maytenus rigida extract

Advanced Microtexture Study of Anacardium occidentale L. Leaf Surface From the Amazon by Fractal Theory

An Abraded Surface of Doxorubicin-Loaded Surfactant-Containing Drug Delivery Systems Effectively Reduces the Survival of Carcinoma Cells

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