FoxO genes are dispensable during gastrulation but required for late embryogenesis in Xenopus laevis

Schuff, Maximilian; Siegel, Doreen; Bardine, Nabila; Oswald, Franz; Donow, Cornelia; Knöchel, Walter

doi:10.1016/j.ydbio.2009.10.036

Cited by 21 publications

(15 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Foxo family TFs, particularly foxo1 and foxo3a, are well known as regulators of cell cycle arrest and apoptosis (Weidinger et al, 2008), but Foxo4 is also implicated in motility. Foxo4 is required for neural crest migration in Xenopus laevis and for in vitro smooth muscle cell migration (Schuff et al, 2010).…”

Section: Motilitymentioning

confidence: 99%

Sub-circuits of a gene regulatory network control a developmental epithelial-mesenchymal transition

2014

View full text Add to dashboard Cite

Epithelial-mesenchymal transition (EMT) is a fundamental cell state change that transforms epithelial to mesenchymal cells during embryonic development, adult tissue repair and cancer metastasis. EMT includes a complex series of intermediate cell state changes including remodeling of the basement membrane, apical constriction, epithelial de-adhesion, directed motility, loss of apical-basal polarity, and acquisition of mesenchymal adhesion and polarity. Transcriptional regulatory state changes must ultimately coordinate the timing and execution of these cell biological processes. A wellcharacterized gene regulatory network (GRN) in the sea urchin embryo was used to identify the transcription factors that control five distinct cell changes during EMT. Single transcription factors were perturbed and the consequences followed with in vivo time-lapse imaging or immunostaining assays. The data show that five different sub-circuits of the GRN control five distinct cell biological activities, each part of the complex EMT process. Thirteen transcription factors (TFs) expressed specifically in pre-EMT cells were required for EMT. Three TFs highest in the GRN specified and activated EMT (alx1, ets1, tbr) and the 10 TFs downstream of those (tel, erg, hex, tgif, snail, twist, foxn2/3, dri, foxb, foxo) were also required for EMT. No single TF functioned in all five sub-circuits, indicating that there is no EMT master regulator. Instead, the resulting sub-circuit topologies suggest EMT requires multiple simultaneous regulatory mechanisms: forward cascades, parallel inputs and positive-feedback lock downs. The interconnected and overlapping nature of the sub-circuits provides one explanation for the seamless orchestration by the embryo of cell state changes leading to successful EMT.

show abstract

Section: Motilitymentioning

confidence: 99%

Sub-circuits of a gene regulatory network control a developmental epithelial-mesenchymal transition

2014

View full text Add to dashboard Cite

show abstract

“…This anti-apoptotic factor was related to an increase of apoptosis in the parts of embryos needing cell death for harmonious development [24]. Several experiments based upon the over-expression of FoxO genes in X. laevis showed these genes were indispensable for tissue differentiation but not for gastrulation, even overexpression of several of them induced severe damages in gastrulae [25]. Bix expressed in early Xenopus gastrula, and an over-expression as well as a depletion of Bix3 causes apoptosis [26].…”

Section: Regulation Of Apoptosis During Segmentation and Gastrulationmentioning

confidence: 99%

Apoptosis in amphibian development

Exbrayat¹,

Moudilou²,

Abrouk³

et al. 2012

ABB

View full text Add to dashboard Cite

Amphibians and more particularly X. laevis are models often used for studying apoptosis during embryonic development. Using several methods, searchers determined the localization of programmed cell deaths (PCD). Several experimental methods also have been used to understand the regulatory mechanisms of apoptosis, throughout development, contributing to elucidate the general action of several genes and proteins. Apoptosis occurs very early, with a first program under control of maternal genes expressed before MBT, in order to eliminate damaged cells before gastrulation, and a second program at the onset of gastrulation. PCD is also observed during neurulation. Then, apoptotic cells are observed in amphibian organogenesis and metamorphosis. Results of these researches showed both importance of PCD for embryonic development, and the complexity of its regulation. Results obtained can be useful to understand others aspects of the importance of apoptosis, particularly pathological aspects.

show abstract

“…Although poor overlap may be partially attributable to differences in the species and/or methods used for gene expression profiling, these studies suggest that FOXO proteins indeed regulate specific targets in different contexts. In addition to being important for stem cell homeostasis and adult longevity, FOXO factors are involved in development and cell differentiation (Kitamura et al, 2007; Demontis and Perrimon, 2009; Yuan et al, 2009; de la Torre-Ubieta et al, 2010; Kerdiles et al, 2010; Schuff et al, 2010), and they may be important to promote adult neurogenesis as suggested by studies in Drosophila melanogaster (Siegrist et al, 2010). Thus, FOXO factors may be essential to cell response homeostasis during the entire lifetime of a living organism.…”

Section: The Foxo Networkmentioning

confidence: 99%

Role and Therapeutic Potential of the Pro-Longevity Factor FOXO and Its Regulators in Neurodegenerative Disease

Néri¹

2012

Front. Pharmacol.

View full text Add to dashboard Cite

Studies in simple model organisms have yielded crucial insights into the genetic and molecular aspects of longevity. FOXO, which is most notable for its association with longevity, and its upstream regulators such as sirtuins have received particular attention in translational research because these genes modulate cell survival in several models of neurodegenerative diseases. There is a large amount of knowledge on the pathways that regulate FOXO activity and genes that may be regulated by FOXO. However, for the same reason that the FOXO network is a complex stress response system, its therapeutic potential to develop disease-modifying strategies requires further examination. Although the FOXO network contains druggable genes such as sirtuins and AMPK, whether they should be activated or inhibited and whether protection against the early or late phases of neuronal cell decline might require opposite therapeutic strategies remains unclear. Additionally, the mode of action of small compound molecules believed to act on FOXO network targets was questioned. This review recapitulates essential facts and questions about the promises of FOXO and its interactors in neurodegenerative disease.

show abstract

FoxO genes are dispensable during gastrulation but required for late embryogenesis in Xenopus laevis

Cited by 21 publications

References 67 publications

Sub-circuits of a gene regulatory network control a developmental epithelial-mesenchymal transition

Sub-circuits of a gene regulatory network control a developmental epithelial-mesenchymal transition

Apoptosis in amphibian development

Role and Therapeutic Potential of the Pro-Longevity Factor FOXO and Its Regulators in Neurodegenerative Disease

Contact Info

Product

Resources

About