Fourier-transform vs. quantum-cascade-laser infrared microscopes for histo-pathology: From lab to hospital?

Ogunleke, Abiodun; Bobroff, Vladimir; Chen, Hsiang‐Hsin; Rowlette, Jeremy; Delugin, Maylis; Recur, Benoît; Hwu, Y.; Petibois, Cyril

doi:10.1016/j.trac.2017.02.007

Cited by 22 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…64,74 In mid-IR microscopy, QCL powered imaging systems allow 150 times faster spectral data acquisition at equivalent SNR at comparable spectral and lateral resolution. 75 For high spectral resolution applications, the field is dominated by DFB-QCLs and EC-QCLs in single-mode operation. 55,76 The narrow-linewidth emission offers high selectivity, high sensitivity and enable multiple-species sensing that is particularly desirable in trace-gas sensing.…”

Section: Specific Properties Of Qcls Relevant For Applied Spectroscopymentioning

confidence: 99%

“…Most recently, it has also been demonstrated, that even with full spectra acquisition, a QCL-based imaging systems may have 150Â faster acquisition times than FTIR microscopes at equivalent signal-to-noise level. 75 In 2014, the first commercial QCL-based IR imaging system became available (Spero, Daylight Solutions Inc., San Diego, CA, USA). After early demonstrations of the feasibility of QCL-based IR microscopy, 183,184 particularly the Bhargava group focussed on development and characterisation of a custom-built setup 59,182 and subsequent application to biomedical and clinical questions.…”

Section: Qcl-ir Imaging For Histopathology and Biofluid Screeningmentioning

confidence: 99%

See 1 more Smart Citation

Quantum cascade lasers (QCLs) in biomedical spectroscopy

2017

View full text Add to dashboard Cite

Quantum cascade lasers (QCL) are the first room temperature semiconductor laser source for the mid-IR spectral region, triggering substantial development for the advancement of mid-IR spectroscopy. Mid-IR spectroscopy in general provides rapid, label-free and objective analysis, particularly important in the field of biomedical analysis. Due to their unique properties, QCLs offer new possibilities for development of analytical methods to enable quantification of clinically relevant concentration levels and to support medical diagnostics. Compared to FTIR spectroscopy, novel and elaborated measurement techniques can be implemented that allow miniaturized and portable instrumentation. This review illustrates the characteristics of QCLs with a particular focus on their benefits for biomedical analysis. Recent applications of QCL-based spectroscopy for analysis of a variety of clinically relevant samples including breath, urine, blood, interstitial fluid, and biopsy samples are summarized. Further potential for technical advancements is discussed in combination with future prospects for employment of QCL-based devices in routine and point-of-care diagnostics.

show abstract

Section: Specific Properties Of Qcls Relevant For Applied Spectroscopymentioning

confidence: 99%

Section: Qcl-ir Imaging For Histopathology and Biofluid Screeningmentioning

confidence: 99%

Quantum cascade lasers (QCLs) in biomedical spectroscopy

2017

View full text Add to dashboard Cite

show abstract

“…In recent years, a number of highly advanced techniques like tip-enhanced Raman spectroscopy (TERS), infrared atomic force microscopy (AFM-IR), and infrared photo-induced force microscopy (IR PiFM), especially, have provided spectroscopic imaging with nano-scale spatial resolution (Levin and Bhargava 2005;Lewis et al 1995;Nguyen et al 2020;Ogunleke et al 2017;Xiao and Schultz 2018). AFM-IR based on thermal expansion of the material has been applied for studying wood cell wall (Wang et al 2016;Gusenbauer et al 2020) and xylem pit membranes (Pereira et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Infrared photo-induced force microscopy unveils nanoscale features of Norway spruce fibre wall

et al. 2021

View full text Add to dashboard Cite

Infrared photo-induced force microscopy (IR PiFM) was applied for imaging ultrathin sections of Norway spruce (Picea abies) at 800–1885 cm−1 with varying scanning steps from 0.6 to 30 nm. Cell wall sublayers were visualized in the low-resolution mode based on differences in their chemical composition. The spectra from the individual sublayers demonstrated differences in the orientation of cellulose elementary fibrils (EFs) and in the content and structure of lignin. The high-resolution images revealed 5–20 nm wide lignin-free areas in the S1 layer. Full spectra collected from a non-lignified spot and at a short distance apart from it verified an abrupt change in the lignin content and the presence of tangentially oriented EFs. Line scans across the lignin-free areas corresponded to a spatial resolution of ≤ 5 nm. The ability of IR PiFM to resolve structures based on their chemical composition differentiates it from transmission electron microscopy that can reach a similar spatial resolution in imaging ultrathin wood sections. In comparison with Raman imaging, IR PiFM can acquire chemical images with ≥ 50 times higher spatial resolution. IR PiFM is also a surface-sensitive technique that is important for reaching the high spatial resolution in anisotropic samples like the cell wall. All these features make IR PiFM a highly promising technique for analyzing the recalcitrant nature of lignocellulosic biomass for its conversion into various materials and chemicals. Graphic abstract

show abstract

“…However, FTIR is currently too slow for real-time imaging for the delivery of rapid hyperspectral pathology. In recent years, discrete frequency IR microscopes with tunable mid-IR laser sources, such as quantum cascade lasers, optical parametric oscillators or filtered supercontinuum light sources, enabling label-free classification of cancerous tissue have been demonstrated [15][16][17][18][19][20]. By tuning to a molecular absorbance wavelength of interest, real-time molecular imaging can be utilized for immediate unstained tissue analysis.…”

Section: Introductionmentioning

confidence: 99%

Upconversion raster scanning microscope for long-wavelength infrared imaging of breast cancer microcalcifications

Tseng¹,

Bouzy²,

Pedersen³

et al. 2018

Biomed. Opt. Express

View full text Add to dashboard Cite

Long-wavelength identification of microcalcifications in breast cancer tissue is demonstrated using a novel upconversion raster scanning microscope. The system consists of quantum cascade lasers (QCL) for illumination and an upconversion system for efficient, high-speed detection using a silicon detector. Absorbance spectra and images of regions of ductal carcinoma in situ (DCIS) from the breast have been acquired using both upconversion and Fourier-transform infrared (FTIR) systems. The spectral images are compared and good agreement is found between the upconversion and the FTIR systems.

show abstract

Fourier-transform vs. quantum-cascade-laser infrared microscopes for histo-pathology: From lab to hospital?

Cited by 22 publications

References 31 publications

Quantum cascade lasers (QCLs) in biomedical spectroscopy

Quantum cascade lasers (QCLs) in biomedical spectroscopy

Infrared photo-induced force microscopy unveils nanoscale features of Norway spruce fibre wall

Upconversion raster scanning microscope for long-wavelength infrared imaging of breast cancer microcalcifications

Contact Info

Product

Resources

About