Fourier Transform Light Scattering of Inhomogeneous and Dynamic Structures

Ding, Huafeng; Wang, Zhuo; Nguyen, Freddy T.; Boppart, Stephen A.; Popescu, Gabriel

doi:10.1103/physrevlett.101.238102

Cited by 145 publications

(148 citation statements)

References 22 publications

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“…Note that this is a departure from the far-zone, angular scattering that is traditionally used in X-ray diffraction. When dealing with transparent objects, measuring the complex field at the image plane yields higher sensitivity than measuring in the far-zone 38 . Third, WDT is implemented using an existing phase contrast microscope with white-light illumination, and the three-dimensional structure is recovered by simply translating the objective lens, which scans the focal plane axially through the specimen.…”

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White-light diffraction tomography of unlabelled live cells

et al. 2014

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We present a technique called white-light diffraction tomography (WDT) for imaging microscopic transparent objects such as live unlabelled cells. The approach extends diffraction tomography to white-light illumination and imaging rather than scattering plane measurements. Our experiments were performed using a conventional phase contrast microscope upgraded with a module to measure quantitative phase images. The axial dimension of the object was reconstructed by scanning the focus through the object and acquiring a stack of phase-resolved images. We reconstructed the threedimensional structures of live, unlabelled, red blood cells and compared the results with confocal and scanning electron microscopy images. The 350 nm transverse and 900 nm axial resolution achieved reveals subcellular structures at high resolution in Escherichia coli cells. The results establish WDT as a means for measuring three-dimensional subcellular structures in a non-invasive and label-free manner.A transparent object illuminated by an electromagnetic field generates a scattering pattern that carries specific information about its internal structure. Inferring this information from measurements of the scattered field, that is, solving the inverse scattering problem, is the fundamental principle that has allowed X-ray diffraction measurements to reveal the molecular-scale organization of crystals 1 and more recently, image cells with nanoscale resolution 2,3 . The scattered field is related to the spatially varying dielectric susceptibility of the scattering object by a transformation that simplifies considerably and, more importantly, becomes invertible, when the incident field is only weakly perturbed by the presence of the object. In this regime, the first-order Born approximation 4 and the Rytov approximation 5 have been used to unambiguously retrieve the three-dimensional spatial distribution of the dielectric constant. Implementation of inverse scattering requires knowledge of both the amplitude and phase of the scattered field. This obstacle, known as the phase problem, has been associated with X-ray diffraction measurement throughout its century-old history (for a review, see ref. 6).In 1969, Wolf proposed diffraction tomography as a reconstruction method combining the X-ray diffraction principle with optical holography 7 . Unlike X-rays, light at lower frequencies can be used in phase imaging measurements, as demonstrated by Gabor 8 . In recent years, as a result of new advances in light sources, detector arrays and computing power, quantitative phase imaging (QPI), in which optical path-length delays are measured at each point in the field of view, has become a very active field of study 9 . Whether involving holographic or non-holographic methods 10-16 , QPI presents new opportunities for studying cells and tissues non-invasively, quantitatively and without the need for staining or tagging [17][18][19][20][21][22][23] . Projection tomography using laser QPI has made use of ideas from X-ray imaging and enabled three-dimensional ...

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White-light diffraction tomography of unlabelled live cells

et al. 2014

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“…3. In order to analyze the behavior of anisotropy at the different wavelengths, we normalized the magnitude squared of the Fourier transform to obtain the probability density map of the angular scattering intensity [23,24] [see Figs. 4(a) and 4(d)].…”

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confidence: 99%

Measurement of multispectral scattering properties in mouse brain tissue

Min

Ban

Wang

et al. 2017

Biomed. Opt. Express

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Abstract:We present the scattering properties of mouse brain using multispectral diffraction phase microscopy. Typical diffraction phase microscopy was incorporated with the broadband light source which offers the measurement of the scattering coefficient and anisotropy in the spectral range of 550-900 nm. The regional analysis was performed for both the myeloarchitecture and cytoarchitecture of the brain tissue. Our results clearly evaluate the multispectral scattering properties in the olfactory bulb and corpus callosum. The scattering coefficient measured in the corpus callosum is about four times higher than in the olfactory bulb. It also indicates that it is feasible to realize the quantitative phase microscope in near infrared region for thick brain tissue imaging.

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“…(2) Even though the particular focus of this study is on static 3-D images of phytoplankton, dynamics of 3-D RI tomograms of individual phytoplankton can allow to measure intracellular dynamics of subcellular organelles [39][40][41] or dynamics fluctuation in cell membrane [42][43][44][45][46][47] which can provide abundant information about pathophysiology of phytoplankton. (3) In addition, recent advances in QPI techniques can also be further employed to investigate phytoplankton research including super-resolution imaging [48], Fourier transform light scattering technique [49][50][51][52][53], real-time visualization of 3-D RI maps [54], multi-spectral QPI [55][56][57][58][59], and polarization-sensitive QPI [60,61]. (4) Furthermore, QPI techniques can be accessible to an existing microscope by attaching the recently developed QPI unit [62,63], which will further extend the applicability of QPI techniques for the study of phytoplankton.…”

Section: Discussionmentioning

confidence: 99%

High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton

Lee¹,

K²,

Mubarok³

et al. 2014

Journal of the Optical Society of Korea

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Optical measurements of the morphological and biochemical imaging of phytoplankton are presented. Employing quantitative phase imaging techniques, 3-D refractive index maps and high-resolution 2-D quantitative phase images of individual live phytoplankton are simultaneously obtained without exogenous labeling agents. In addition, biochemical information of individual phytoplankton including volume, mass, and density of individual phytoplankton are also quantitatively obtained from the measured refractive index distributions. We expect the present method to become a powerful tool for the study of phytoplankton.

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Fourier Transform Light Scattering of Inhomogeneous and Dynamic Structures

Cited by 145 publications

References 22 publications

White-light diffraction tomography of unlabelled live cells

White-light diffraction tomography of unlabelled live cells

Measurement of multispectral scattering properties in mouse brain tissue

High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton

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