Four SQUAMOSA PROMOTER BINDING PROTEIN-LIKE homologs from a basal eudicot tree (Platanus acerifolia) show diverse expression pattern and ability of inducing early flowering in Arabidopsis

Han, Hongji; Li, Guofeng; Zhang, Jiaqi; Zhang, Sisi; Cai, Fangfang; Bao, Zhiru; Zhang, Yanping; Bao, Manzhu

doi:10.1007/s00468-016-1378-5

Cited by 8 publications

(3 citation statements)

References 35 publications

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“…The SQUAMOSA promoter-binding protein-LIKE (SPL) family of transcription factors (TFs) integrate multiple pathways 21–25 . SPLs have been shown to regulate flowering time and flower organ development in both herbs and woody plants, such as Gossypium hirsutum 26 , maize 27 , birch 28 , Prunus mume 29 , and Platanus acerifolia 30 . In the model plant Arabidopsis, AtSPLs have been shown to be a group of dominant regulators of the flowering process 4, 11, 13, 22, 24, 31–34 .…”

Section: Introductionmentioning

confidence: 99%

Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei

Wang

Zhu

Fan

et al. 2019

Preprint

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15"Bairihua", a variety of the Catalpa bungei, has a large amount of flowers and a long flowering 16 period which make it an excellent material for flowering researches in trees. SPL is one of the hub 17 genes that regulate both flowering transition and development. Here, a SPL homologues CbuSPL9 18 was cloned using degenerate primers with RACE. Expression studies during flowering transition 19in Bairihua and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly 20 with its Arabidopsis homologues. In the next step, we used Y2H to identify the proteins that could 21 interact with CbuSPL9. HMGA, an architectural transcriptional factor, was identified and cloned 22 for further research. BiFC and BLI showed that CbuSPL9 could form a heterodimer with 23CbuHMGA in the nucleus. The expression analysis showed that CbuHMGA had a similar 24 expression trend to that of CbuSPL9 during flowering in "Bairihua". Intriguingly, ectopic 25 expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect 26 flowering time. Taken together, our results implied a novel pathway that ChuSPL9 regulated 27 flowering development, but not flowering transition, with the participation of ChuHMGA. Further 28 investments need to be done to verify the details of this pathway. 29 Keywords: Catalpa bungei; Flowering; SPL; HMGA; Architectural transcriptional factor. 30 bungei is valuable as both a timber and an ornamental tree 1 . "Bairihua", which is a natural variety 37 of C. bungei, has been characterized for its especially short juvenile period, large number of 38 flowers and long flowering period. The flowering period of "Bairihua" is approximately 15 days, 39 and its accumulative flowering period reaches 100 days, which is very rare for woody plants 40(http://www.forestry.gov.cn/). "Bairihua" provides an excellent opportunity to evaluate the 41 flowering process of woody plants. 42Flowering is controlled by sophisticated regulatory networks 2-5 . Five major pathways are involved 43 in these processes, including the aging pathway 6 , gibberellin pathway 7-11 , photoperiod 44 pathway 12-16 , vernalization pathway [17][18][19] and autonomous pathway 20 . The SQUAMOSA 45 promoter-binding protein-LIKE (SPL) family of transcription factors (TFs) integrate multiple 46 pathways [21][22][23][24][25] . SPLs have been shown to regulate flowering time and flower organ development in 47 both herbs and woody plants, such as Gossypium hirsutum 26 , maize 27 , birch 28 , Prunus mume 29 , and 48Platanus acerifolia 30 . In the model plant Arabidopsis, AtSPLs have been shown to be a group of 49 dominant regulators of the flowering process 4,11,13,22,24,[31][32][33][34] . The overexpression of AtSPLs leads to 50 early flowering and abnormal inflorescence, and conversely, the inhibition of AtSPL expression 51 delays the occurrence of floral transition 21,[35][36][37] . As a group of TFs, SPLs regulate the expression 52 of other genes. Numerous downstream genes of SPLs have been identified; for example, AtSPL3 53 can...

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Section: Introductionmentioning

confidence: 99%

Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei

Wang

Zhu

Fan

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The SQUAMOSA promoter-binding protein-LIKE (SPL) family of transcription factors (TFs) integrate multiple pathways [18][19][20][21][22]. SPLs have been shown to regulate flowering time and flower organ development in both herbs and woody plants, such as Gossypium hirsutum [23], maize [24], birch [25], Prunus mume [26], Platanus acerifolia [27], and Populus trichocarpa [28]. In the model plant Arabidopsis, AtSPLs have been shown to be a group of dominant regulators of the flowering process [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei

Wang

Zhu

et al. 2020

BMC Plant Biol

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Background: "Bairihua", a variety of the Catalpa bungei, has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flowering transition and development. Results: SPL homologues CbuSPL9 was cloned using degenerate primers with RACE. Expression studies during flowering transition in "Bairihua" and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly with its Arabidopsis homologues. In the next step, we used Y2H to identify the proteins that could interact with CbuSPL9. HMGA, an architectural transcriptional factor, was identified and cloned for further research. BiFC and BLI showed that CbuSPL9 could form a heterodimer with CbuHMGA in the nucleus. The expression analysis showed that CbuHMGA had a similar expression trend to that of CbuSPL9 during flowering in "Bairihua". Intriguingly, ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time. Conclusions: Our results implied a novel pathway that CbuSPL9 regulated flowering development, but not flowering transition, with the participation of CbuHMGA. Further investments need to be done to verify the details of this pathway.

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“…Three homologous genes, AtSPL13 , TEOSINTE GLUME ARCHITECTURE 1 ( TGA1 ), and OsSPL6 , all played critical roles in the vegetative and reproductive phases: AtSPL13 gene has been shown previously to affect the initiation of the first true leaves [ 28 ], maize tga1 was involved in ear glume development [ 29 ], and OsSPL16 controlled grain size, shape, and quality in rice [ 30 ]. Moreover, the petunia gene PhSBP1 can accelerate leaf initiation rate and control the timing of developmental phase change [ 22 ], while PaSPL3a/b/c/d from Platanus acerifolia were found to induce early flowering and control vegetative to reproductive phase change [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

The SBP-Box Gene VpSBP11 from Chinese Wild Vitis Is Involved in Floral Transition and Affects Leaf Development

Hou

Yan

Sha

et al. 2017

IJMS

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Flowering occurs in angiosperms during a major developmental transition from vegetative growth to the reproductive phase. Squamosa promoter binding protein (SBP)-box genes have been found to play critical roles in regulating flower and fruit development, but their roles in grapevine have remained unclear. To better understand the functions of the grape SBP-box genes in both vegetative and reproductive growth phases, a full-length complementary DNA (cDNA) sequence of the putative SBP-box transcription factor gene, VpSBP11, was obtained from Chinese wild grapevine Vitis pseudoreticulata Wen Tsai Wang (W. T. Wang) clone ‘Baihe-35-1’. VpSBP11 encoded a putative polypeptide of 170 amino acids with a highly conserved SBP-domain with two zinc-binding sites of the Cx2C-x3-H-x11-C-x6-H (C2HCH) type and a nuclear localization signal. We confirmed that the VpSBP11 protein was targeted to the nucleus and possessed transcriptional activation activity by subcellular localization and trans-activation assay. Over-expression of VpSBP11 in Arabidopsis thaliana was shown to activate the FUL gene, and subsequently the AP1 and LFY genes, all of which were floral meristem identity genes, and to cause earlier flowering than in wild type (WT) plants. The pattern of vegetative growth was also different between the transgenic and WT plants. For example, in the VpSBP11 over-expressing transgenic plants, the number of rosette leaves was less than that of WT; the petiole was significantly elongated; and the rosette and cauline leaves curled upwards or downwards. These results were consistent with VpSBP11 acting as a transcription factor during the transition from the vegetative stage to the reproductive stage.

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Four SQUAMOSA PROMOTER BINDING PROTEIN-LIKE homologs from a basal eudicot tree (Platanus acerifolia) show diverse expression pattern and ability of inducing early flowering in Arabidopsis

Cited by 8 publications

References 35 publications

Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei

Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei

Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei

The SBP-Box Gene VpSBP11 from Chinese Wild Vitis Is Involved in Floral Transition and Affects Leaf Development

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