Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei

Abstract

Background: "Bairihua", a variety of the Catalpa bungei, has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flowering transition and development. Results: SPL homologues CbuSPL9 was cloned using degenerate primers with RACE. Expression studies during flowering transition in "Bairihua" and ectopic expression in Arabidopsis showed that CbuSPL9 was functional similarly with its Arabidopsis homologues. In the next step, we used Y2H to identify the proteins that could interact with CbuSPL9. HMGA, an architectural transcriptional factor, was identified and cloned for further research. BiFC and BLI showed that CbuSPL9 could form a heterodimer with CbuHMGA in the nucleus. The expression analysis showed that CbuHMGA had a similar expression trend to that of CbuSPL9 during flowering in "Bairihua". Intriguingly, ectopic expression of CbuHMGA in Arabidopsis would lead to aberrant flowers, but did not effect flowering time. Conclusions: Our results implied a novel pathway that CbuSPL9 regulated flowering development, but not flowering transition, with the participation of CbuHMGA. Further investments need to be done to verify the details of this pathway.

Genome-Wide Identification and Expression Analysis of the SQUAMOSA Promoter-Binding Protein-like (SPL) Transcription Factor Family in Catalpa bungei

“…Catalpa 'Bairihua' is an excellent hybrid with multi-season flowering obtained through the hybrid breeding of Catalpa bungei 'Luo Qiu 4 and C. fargesii Bur. f. duclouxii (http://rif.caf.ac.cn/News.aspx?ItemID=6009, accessed on 20 June 2022) [25,26]. Moreover, it can bloom in the same year through asexual reproduction methods such as grafting, which is very rare in woody plants [25].…”