Four pentacyclic triterpenoids with antifungal and antibacterial activity from Curtisia dentata (Burm.f) C.A. Sm. leaves

Shai, Leshweni Jeremia; McGaw, Lyndy Joy; Aderogba, M.A.; Mdee, L. K.; Eloff, Jacobus Nicolaas

doi:10.1016/j.jep.2008.06.036

Cited by 131 publications

(98 citation statements)

References 33 publications

(35 reference statements)

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“…The hexane and dichloromethane extracts of Curtisia dentata (Burm.f) were more toxic than the aqueous extracts of M. obtusifolia in this study (Shai et al 2008). Other evidence suggests that ethanol extracts are more cytotoxic than aqueous extracts of plants.…”

Section: Discussionsupporting

confidence: 43%

Anthelmintic and cytotoxic activities of extracts of Markhamia obtusifolia Sprague (Bignoniaceae)

Nchu¹,

Githiori²,

McGaw³

et al. 2011

Veterinary Parasitology

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The anthelmintic activity of Markhamia obtusifolia Sprague (Bignoniaceae) leaf extracts was evaluated against the ruminant gastrointestinal nematode parasite Trichostrongylus colubriformis (Nematoda: Strongylida) using the in vitro egg hatch test. Also, the cytotoxic activity of aqueous extracts of M. obtusifolia was evaluated in cell line cytotoxicity assays. The results indicated that the effective concentration (EC 50 ) for the water extract of M. obtusifolia leaves (0.46 mg/mL; Confidence Interval[CI] 0.3-0.5 mg/mL) was significantly lower than the EC 50 for the acetone extract of M. obtusifolia (0.8 mg/mL; CI 0.7-1 mg/mL). Aqueous extracts were twice as potent as the acetone extracts. The ED 90 (0.2 mg/mL; CI 0.1-0.02) for thiabendazole (positive control) was significantly lower than the EC 90 for the water extract of M. obtusifolia 2 (10.7 mg/mL; CI 8.3-13.7 mg/mL). In the cytotoxicity bioassay, the lethal concentration (LC 50 ) for the aqueous extract of M. obtusifolia was 0.476 mg/mL, which was relatively high (low toxicity) in comparison to the highly toxic berberine (LC 50 = 9.80 µg/mL). The current study showed that M. obtusifolia plant extracts possess anthelmintic activity and are relatively non-cytotoxic, thus providing support for their use in traditional veterinary practices.

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Section: Discussionsupporting

confidence: 43%

Anthelmintic and cytotoxic activities of extracts of Markhamia obtusifolia Sprague (Bignoniaceae)

Nchu¹,

Githiori²,

McGaw³

et al. 2011

Veterinary Parasitology

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“…The high MIC values (100 to 2500 mg/ml) recorded for the aqueous extracts compared to the ethanol extracts may not necessarily mean that the extracts do not posses antimicrobial activity, but that the phytoconstituents may be present in very low amounts at the tested concentrations. Antimicrobial activity of the acetone extracts of leaves, twigs and stem barks of C. dentata against Bacillus subtilis, E. coli, S. aureus, P. aerugenosa, E. faecalis and C. albicans as well as inhibition of motility in some parasitic and free living nematodes has earlier been reported (McGaw et al, 2000;Shai et al 2008Shai et al , 2009). This however, is the first report on the activity of C. dentata on verotoxic bacteria.…”

Section: Discussionmentioning

confidence: 93%

Virulence factors and antibiotic susceptibility among verotoxic non O157: H7 Escherichia coli isolates obtained from water and wastewater samples in Cape Town, South Africa.

Doughari¹,

Ndakidemi²,

Human³

et al. 2011

Afr. J. Biotechnol.

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Forty eight samples (30 wastewater and 18 river water) were collected between July and November, 2010, from different sources in Cape Town, South Africa in order to characterize verotoxic non O157: H7 Escherichia coli strains. Samples (1 ml) were inoculated into MacConkey broth (MB, 9 ml) and incubated at 37°C for 24 h, after which a loopful of the MB was then spread onto Eosin Methylene Blue (EMB) and further incubated for 24 h at 37°C in order to isolate E. coli. The identification of isolates was done using standard biochemical procedures; and confirmed serologically using E. coli polyvalent antisera (Bioweb, SA). Isolates were also characterized for virulence factors such as verotoxin, haemolysin, gelatinase, extended spectrum beta lactamases (ESBLs), cell surface hydrophobicity and bacterial serum resistance, as well as susceptibility (using disc diffusion method) to stem bark extracts of Curtisia dentata. Results showed the presence of different serotypes of E. coli (69 isolates in all) including 026: H11, 055, O111: NM, O126, O44, O124, O96:H9, O103:H2, O145: NM and O145:H2. Over 60% of the isolates exhibited serum resistance, haemolysin and gelatinase production, 81% exhibited cell surface hydrophobicity and over 52% produced ESBLs. Results also showed that while 60% of the isolates showed various levels of resistance to different antibiotics [ampicillin (10 µg), cefuroxime, cephalexin, ceftazidime and tetracycline (30 µg in each case) (multidrug resistance index (MDRI) values 4.20 to 5.60%)], only 28% were resistant to ethanol stem bark extracts of C. dentata (MIC, 70 to 150 mg/ml). The presence of pathogenic verotoxic antibiotic resistant E. coli in these water sources is a threat to water quality and food security and C. dentata has a potential for sourcing novel antibiotic substances for chemotherapy against these resistant pathogenic strains of E. coli.

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“…Oleanolic acid, for example, was reported to exhibit antibacterial activity against Gram-negative bacteria by alteration of bacterial shape and inhibition of bacterial growth and survival [50]. Lupeol was reported in another study to exhibit antibacterial activity against Escherichia coli [51]. The activity of B. luridum total extract against Salmonella typhi might be due to the synergistic action of all compounds isolated from the plant such as triterpenes and flavonoids [11].…”

Section: Discussionmentioning

confidence: 99%

Anti-infective Properties of Brachychiton rupestris and Brachychiton luridum Leaves and their Qualitative Phytochemical Screening

Marasabessy¹,

Rudiyono²,

Dewi³

2017

Med Aromat Plants

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Brachychiton rupestris and Brachychiton luridum are deciduous trees belonging to family Malvaceae. Several members of the genus Brachychiton and its related genus, Sterculia, are used in folk medicine for the treatment of infections. Herein, we investigated the antimicrobial activity of the total extracts, n-hexane, dichloromethane as well as the ethyl acetate fractions of the leaves of both species using agar well diffusion method in an effort to better consolidate the scientific basis for their traditional uses. The study was carried out against two Gram-positive and two Gram-negative bacteria together with two fungi. The extracts and fractions showed mean inhibition zones diameter in the range of 10.97-14.98 mm with minimum inhibitory concentration values ranging between 39.06 and 625 µg/mL against the susceptible bacteria and fungi. The ethyl acetate fraction of B. luridum exhibited notable activity against Staphylococcus aureus while the total extract showed moderate activity against Salmonella typhi. Regarding B. rupestris, the total extract, n-hexane and ethyl acetate fractions showed moderate activity against Escherichia coli, Candida albicans, and Salmonella typhi, respectively. Other extracts and fractions showed weak or no activity against the tested microorganisms. Qualitative phytochemical screening of both plants was performed to preliminary unveil the classes of secondary metabolites present in the leaves. The results indicated the existence of flavonoids, tannins, sterols, triterpenes, carbohydrates and/or glycosides in both plants.

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Four pentacyclic triterpenoids with antifungal and antibacterial activity from Curtisia dentata (Burm.f) C.A. Sm. leaves

Cited by 131 publications

References 33 publications

Anthelmintic and cytotoxic activities of extracts of Markhamia obtusifolia Sprague (Bignoniaceae)

Anthelmintic and cytotoxic activities of extracts of Markhamia obtusifolia Sprague (Bignoniaceae)

Virulence factors and antibiotic susceptibility among verotoxic non O157: H7 Escherichia coli isolates obtained from water and wastewater samples in Cape Town, South Africa.

Anti-infective Properties of Brachychiton rupestris and Brachychiton luridum Leaves and their Qualitative Phytochemical Screening

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