Abstract:Four new irisflavones A-D (1-4) and irilin D (5) have been isolated from the underground parts of Iris bungei along with known isoflavones, irilins A-B (6-7) and tlatancuayin (8). The structures of the new compounds were determined using NMR and mass spectroscopic methods and were found to be 2',5,7-trihydroxy-3,6-dimethoxyflavone (1), 2',5-dihydroxy-3,6,7-trimethoxyflavone (2), 2',5,6'-trihydroxy-3,6,7-trimethoxyflavone (3), 3,3',5-trihydroxy-2',7-dimethoxyflavone (4), and 3',4',5,7-tetrahydroxy-6-methoxyisof… Show more
“…The known compounds 3-14 were characterized by comparison of physical and spectral data with the literature values (Jaipetch et al, 1983;Shawl and Kumar, 1992;Inoshiri et al, 1988;Hanawa et al, 1991;Elliger and Halloin, 1994;Jong and Hwang, 1995;Sadikum et al, 1996;Shawl et al, 1984;Kojima et al, 1997;Choudhary et al, 2001b;Prawat et al, 1995). Known compounds were first time isolated from this plant species.…”
“…The known compounds 3-14 were characterized by comparison of physical and spectral data with the literature values (Jaipetch et al, 1983;Shawl and Kumar, 1992;Inoshiri et al, 1988;Hanawa et al, 1991;Elliger and Halloin, 1994;Jong and Hwang, 1995;Sadikum et al, 1996;Shawl et al, 1984;Kojima et al, 1997;Choudhary et al, 2001b;Prawat et al, 1995). Known compounds were first time isolated from this plant species.…”
“…Checking the sequence of the methylene and oxymethine carbon by twodimensional shift correlation spectroscopy, the structure of compound 2 was determined to be a 3-deoxo-derivative of compound 1. On the basis of 1 H and 13 C-NMR data and direct comparison of spectral data from the literature [27][28][29][30][31], structures of isolated compounds were determined (Fig. 5).…”
This study employed the online HPLC-2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)(+) bioassay to rapidly determine the antioxidant compounds occurring in the crude extract of Alnus japonica. The negative peaks of the ABTS(+) radical scavenging detection system, which indicated the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The ABTS(+)-based antioxidant activity profile showed that three negative peaks exhibited antioxidant activity. High-speed counter-current chromatography (HSCCC) was used for preparative scale separation of the three active peaks from the extract. The purity of the isolated compounds was analyzed by HPLC and their structures were identified by (1)H- and (13)C-nuclear magnetic resonance spectrometry (NMR), heteronuclear multiple bond correlation (HMBC), and heteronuclear single quantum correlation (HSQC). Two solvent systems composed of n-hexane/ethylacetate/methanol/water (4:6:4:6, v/v) and of ethyl acetate/methanol/water (1:0.1:1, v/v) were performed in high-speed counter-current chromatography. Consequently, a total of 527 mg of hirsutanonol 5-O-β-D-glucopyranoside, 80.04 mg of 3-deoxohirsutenonol 5-O-β-D-glucopyranoside, and 91.0 mg of hirsutenone were obtained with purity of 94.7, 90.5, and 98.6%, respectively.
“…Among the new isoflavone glycosides isolated from the genus Iris during the reporting period, there are five containing monosaccharides, nine containing disaccharides and one containing trisaccharides. 9,10,11,12,13 Generally, the control of plant diseases is well established with synthetic fungicides.…”
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