Founder effects and stochastic dispersal at the continental scale of the fungal pathogen of bananas <i>Mycosphaerella fijiensis</i>

Rivas, Gonzalo Galilaeo; Zapater, Marie-Françoise; Abadie, Catherine; Carlier, Jean

doi:10.1046/j.1365-294x.2003.02043.x

Cited by 77 publications

(101 citation statements)

References 40 publications

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“…The molecular data supports the anthropogenic movement of T. destructans from Indonesia via infected germplasm or mother plants, most likely linked to the rapidly growing eucalypt plantation industry. A similar founder effect was observed in the genetic structure of Mycosphaerella fijiensis in the Latin America-Caribbean region and Africa, and was attributed to the movement of infected plant material by anthropogenic activities such as food wrapping with infected leaves (Rivas et al 2004). …”

Section: Discussionsupporting

confidence: 52%

Plants for planting; indirect evidence for the movement of a serious forest pathogen, Teratosphaeria destructans, in Asia

Andjic¹,

Dell

Barber

et al. 2011

Eur J Plant Pathol

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Fungal diseases caused by native pathogens and pathogens introduced with planting stock have a significant impact on exotic plantation forestry in the tropics. Teratosphaeria destructans (formerly Kirramyces destructans) is a serious pathogen causing leaf, bud and shoot blight diseases of Eucalyptus spp. in plantations in the sub-tropics and tropics of south-east Asia. This pathogen was first discovered in Indonesia in 1995 and has subsequently spread to Thailand, China, Vietnam and East Timor. The biology, ecology and genetics of this important pathogen have not been explored yet. The objective of this study was, thus, to determine the genetic diversity and movement of T. destructans throughout south-east Asia using multi-gene phylogenies and microsatellite markers. Out of nine gene regions only two microsatellite markers detected a very low nucleotide polymorphism between isolates; seven other gene regions, ITS, β-tubulin, EF1-α, CHS, ATP6 and two microsatellite loci, reflected genetic uniformity. The two polymorphic molecular markers resolved six haplotypes among isolates from Indonesia and only a single haplotype elsewhere in Asia. The low diversity observed among isolates in the region of the first outbreak is as expected for a small founder population. The spread of a single clone over large distances throughout the region supports the hypothesis of spread via the human-mediated movement of germplasm.

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Section: Discussionsupporting

confidence: 52%

Plants for planting; indirect evidence for the movement of a serious forest pathogen, Teratosphaeria destructans, in Asia

Andjic¹,

Dell

Barber

et al. 2011

Eur J Plant Pathol

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“…The first, from the West Coast via the Democratic Republic of Congo and the second from an outbreak on the island of Pemba which quickly moved via Zanzibar to the coastal regions of Tanzania and Kenya and spread from there. The most recent detection of black Sigatoka in the region was on the island of Madagascar in 2000 (Rivas et al 2004).…”

Section: Mycosphaerella Fijiensis (Causing Black Sigatoka)mentioning

confidence: 99%

Black Sigatoka disease: new technologies to strengthen eradication strategies in Australia

Jm¹,

Pattemore²,

Porchun³

et al. 2006

Austral. Plant Pathol.

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Abstract. In 2001, an incursion of Mycosphaerella fijiensis, the causal agent of black Sigatoka, was detected in Australia's largest commercial banana growing region, the Tully Banana Production Area in North Queensland. An intensive surveillance and eradication campaign was undertaken which resulted in the reinstatement of the disease-free status for black Sigatoka in 2005. This was the first time black Sigatoka had ever been eradicated from commercial plantations. The success of the eradication campaign was testament to good working relationships between scientists, growers, crop monitors, quarantine regulatory bodies and industry. A key contributing factor to the success was the deployment of a PCR-based molecular diagnostic assay, developed by the Cooperative Research Centre for Tropical Plant Protection (CRCTPP). This assay complemented morphological identification and allowed high throughput diagnosis of samples facilitating rapid decision-making during the eradication campaign. This paper describes the development and successful deployment of molecular diagnostics for black Sigatoka. Shortcomings in the gel-based assay are discussed and the advantages of highly specific real-time PCR assays, capable of differentiating between Mycosphaerella fijiensis, Mycosphaerella musicola and Mycosphaerella eumusae are outlined. Real-time assays may provide a powerful diagnostic tool for applications in surveillance, disease forecasting and resistance testing for Sigatoka leaf spot diseases.

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“…The genetic variability of M. fijiensis populations in other banana-producing countries has been analyzed using several molecular markers, including restriction fragment length polymorphisms (RFLPs) (Carlier et al, 1996;Hayden et al, 2003), amplified fragment length polymorphisms (AFLPs) (Nakyanzi, 2002), polymerase chain reaction-RFLPs (PCRRFLPs) (Rivas et al, 2004), and variable number of tandem repeats (VNTRs) (Garcia et al, 2010). High polymorphism rates have been the main argument for the use of simple sequence repeats (SSRs) as molecular markers in studies investigating genetic variability in several species (Karaoglu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Virulence and genetic diversity among isolates of Mycosphaerella fijiensis in two regions of Brazil

Silva

Santos

Sousa³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Black sigatoka, caused by the fungus Mycosphaerella fijiensis (anamorphic stage: Paracercospora fijiensis), was first detected in Brazil in early 1998 in the Benjamin Constant and Tabatinga municipalities in the State of Amazonas, near to where the borders of Brazil, Colombia, and Peru converge. Understanding how cultivars react to the pathogen, and characterizing the genetic variability of isolates from two distant and distinct banana-producing regions, are important for determining the virulence of M. fijiensis. In the present study, the genetic diversity of 22 M. fijiensis isolates was assessed using simple sequence repeats (SSR) markers, and their virulence was determined following inoculation on three different banana tree cultivars. All 22 isolates caused symptoms of the disease in the Maçã and Prata Comum cultivars 45 days after inoculation, and at least two virulence groups were identified for the Maçã and Prata Comum cultivars. For the D'Angola cultivars, two virulence groups were observed only after 60 days post-inoculation, and three of the isolates were not virulent. Using SSR markers, the isolates from two different regions of Brazil were placed into two genetic groups, both genetically distant from the Mf 138 isolate collected in Leticia, Colombia. There was no evidence of correlation between the virulence groups and the genetic diversity groups. These results demonstrate variability in virulence between isolates as measured by the severity of black sigatoka in the analyzed cultivars.

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Founder effects and stochastic dispersal at the continental scale of the fungal pathogen of bananas Mycosphaerella fijiensis

Cited by 77 publications

References 40 publications

Plants for planting; indirect evidence for the movement of a serious forest pathogen, Teratosphaeria destructans, in Asia

Plants for planting; indirect evidence for the movement of a serious forest pathogen, Teratosphaeria destructans, in Asia

Black Sigatoka disease: new technologies to strengthen eradication strategies in Australia

Virulence and genetic diversity among isolates of Mycosphaerella fijiensis in two regions of Brazil

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