Fos and jun in rat central amygdaloid nucleus and paraventricular nucleus after stress

Honkaniemi, Jari; Kainu, Tommi; Ceccatelli, Sandra; Rechardt, Leena; Hökfelt, Tomas; Pelto‐Huikko, Markku

doi:10.1097/00001756-199210000-00007

Cited by 78 publications

(42 citation statements)

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“…In young animals the maximum activation occurred 150 min after the onset of acute restraint, while in aged rats the peak value (though still insignificant) was obtained 90 min after stress. The late peak of activation observed in our experiment in young animals is in contrast to results from a study using capsaicin [21], where a positive correlation between time and neuronal activation in the central nuclei of amygdala and paraventricular nuclei of the hypothalamus was found. This discrepancy might be due to the different nature of the stimulus, restraint being a processive stimulus [15] and capsaicin representing a systemic challenge.…”

Section: Discussioncontrasting

confidence: 99%

The effect of age on the dynamics and the level of c-Fos activation in response to acute restraint in Lewis rats

Meyza

Boguszewski

Nikolaev

et al. 2007

Behavioural Brain Research

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Recent studies have reported an age-related increase of anxiety in rodents with a concomitant decrease in neuronal activity in some of the key structures of the fear/anxiety circuit. In the present study we present evidence that distinct parts of this circuit are differentially affected by age in Lewis rats. The effect of ageing is observed both at the actual level of neuronal activation and its time-course. While the structures belonging to the HPA axis react with a bigger neuronal activation and almost no change in the shape of dynamics curve in response to restraint, the structures involved in higher processing of emotional cues (amygdala and hippocampus) become deficiently activated with age despite their generally higher basal level of activation.

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Section: Discussioncontrasting

confidence: 99%

The effect of age on the dynamics and the level of c-Fos activation in response to acute restraint in Lewis rats

Meyza

Boguszewski

Nikolaev

et al. 2007

Behavioural Brain Research

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“…Structures showing increases also show elevations after exposure to stressors such as immobilization or noxious stimulation (Ceccatelli et al, 1989;Honkaniemi et al, 1992;Pezzone et al, 1992;Senba et al, 1993). Similarities and differences in the c-.fos mRNA levels and distribution patterns resulting from cytokines versus stressors deserve further careful analysis.…”

Section: Discussion Endocrine Parametersmentioning

confidence: 99%

Systemic interleukin-1 induces early and late patterns of c-fos mRNA expression in brain

et al. 1994

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This study was designed to examine the mechanisms by which systemic interleukin-1 affects neuroendocrine systems in the brain. Intraperitoneal injections of interleukin-1 beta (1.25 micrograms/rat) were administered to rats. One or three hours after injection, the expression levels of the immediate-early gene c-fos and of genes for several neuropeptides, receptors, and enzymes were examined by in situ hybridization histochemistry. In the brainstem at 1 hr, c-fos mRNA was elevated in the area postrema and nucleus of the solitary tract, but not in the locus coeruleus. At 3 hr, the c-fos mRNA levels had increased further in the nucleus of the solitary tract. Rostrally, elevations in c-fos mRNA levels were found in the hypothalamic and thalamic paraventricular nuclei, central nucleus of amygdala, bed nucleus of the stria terminalis, and medial preoptic area, peaking at 1 hr and diminishing at 3 hr. In addition, at 3 hr a new pattern of c-fos activity emerged--the arcuate nucleus and cells at the external margins throughout the brain now expressed c-fos mRNA. Corticotropin-releasing hormone mRNA levels were doubled in the paraventricular nucleus at 1 and 3 hr, concomitant with elevations in plasma adrenocorticotrophic hormone (ACTH) and corticosterone. Tyrosine hydroxylase mRNA levels in the brainstem did not change. The c-fos mRNA induction patterns reveal a temporally dynamic response to interleukin-1 administration. We propose that the early set of structures responding to interleukin-1 initiates the neuroendocrine response to cytokines. Coactivation of the area postrema and nucleus of the solitary tract may reflect entry into the brain and neural transduction of the peripheral signal. The late set--including the nucleus of the solitary tract, arcuate nucleus, and the brain's edge--may reflect cellular activation along the diffusion routes traveled by interleukin-1 or a bioactive transduction product, because the pattern of edge labeling is similar to the autoradiographic pattern of flow lf radiolabeled tracer substances in the cerebrospinal fluid. The late c-fos mRNA response to interleukin-1, therefore, may represent a demonstration of information transfer in the parasynaptic mode, also known as volume transmission.

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“…The observation that multiple and highly conserved AP-1 binding sites are present within ECR1 is interesting as a number of previous studies have described the expression of fos and jun in the amygdala. 56,57 Further studies have determined that, in parallel with the expression of PPTA, both the expression and activity of fos and jun are unregulated in the amygdala in response to stress. 57,58 In addition, the highly conserved binding sites of the C/EBP protein are represented twice in ECR1.…”

Section: Discussionmentioning

confidence: 99%

A remote and highly conserved enhancer supports amygdala specific expression of the gene encoding the anxiogenic neuropeptide substance-P

et al. 2006

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The neuropeptide substance P (SP), encoded by the preprotachykinin-A (PPTA) gene, is expressed in the central and medial amygdaloid nucleus, where it plays a critical role in modulating fear and anxiety related behaviour. Determining the regulatory systems that support PPTA expression in the amygdala may provide important insights into the causes of depression and anxiety related disorders and will provide avenues for the development of novel therapies. In order to identify the tissue specific regulatory element responsible for supporting expression of the PPTA gene in the amygdala, we used long-range comparative genomics in combination with transgenic analysis and immunohistochemistry. By comparing human and chicken genomes, it was possible to detect and characterise a highly conserved long-range enhancer that supported tissue specific expression in SP expressing cells of the medial and central amygdaloid bodies (ECR1; 158.5 kb 5 0 of human PPTA ORF). Further bioinformatic analysis using the TRANSFAC database indicated that the ECR1 element contained multiple and highly conserved consensus binding sequences of transcription factors (TFs) such as MEIS1. The results of immunohistochemical analysis of transgenic lines were consistent with the hypothesis that the MEIS1 TF interacts with and maintains ECR1 activity in the central amygdala in vivo. The discovery of ECR1 and the in vivo functional relationship with MEIS1 inferred by our studies suggests a mechanism to the regulatory systems that control PPTA expression in the amygdala. Uncovering these mechanisms may play an important role in the future development of tissue specific therapies for the treatment of anxiety and depression.

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Fos and jun in rat central amygdaloid nucleus and paraventricular nucleus after stress

Cited by 78 publications

References 0 publications

The effect of age on the dynamics and the level of c-Fos activation in response to acute restraint in Lewis rats

The effect of age on the dynamics and the level of c-Fos activation in response to acute restraint in Lewis rats

Systemic interleukin-1 induces early and late patterns of c-fos mRNA expression in brain

A remote and highly conserved enhancer supports amygdala specific expression of the gene encoding the anxiogenic neuropeptide substance-P

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