Formulation of Transfersomal Green Tea (Camellia sinensis L. Kuntze) Leaves Extract Cream and In Vitro Penetration Study Using Franz Difussion Cell

Abstract

Introduction: Green tea is a natural ingredient that contains polyphenolic compounds in the form of catechin derivatives known as epigallocatechin gallate (EGCG). It has an antioxidant activity that is beneficial for body health. However, EGCG has a considerable molecular weight and high hydrophilicity resulting in the difficulty of skin penetration. The aim of this study was to improve EGCG penetration through the skin by using the formation of lipid nanovesicles, called transfersomes, that was formulated into a cream dosage form. Methods: Transfersomes were prepared by thin layer hydration method in three formulations with different concentration of phospholipid and surfactant. The amount of green tea leaves extract used for all formulations was same, (equal to 3% EGCG). All transfersomes formulation was characterized their physical properties, and the most promising formulation was chosen to be formulated into a cream dosage form. A cream contained untreated extract was prepared as a control. The in vitro penetration study of EGCG from both creams was evaluated using Franz diffusion cells with the abdomen skin of SpragueDawley rats as a membrane between the compartments. Results and Discussion: The results showed that F1 was the most promising and optimal formulation with a spherical shape, particle size of 80.6 nm, a polydispersity index of 0.214, a zeta potential at -48.2 ± 1.78 mV, and the highest entrapment efficiency of 49.36 ± 4.03%. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

Enhancement strategies for transdermal drug delivery systems: current trends and applications

“…7 ). Recent studies have shown that cilnidipine [ 124 ], diflunisal [ 125 ], sinomenine [ 126 ], green tea extract [ 127 ], pentoxifylline [ 128 ], raloxifene [ 129 ] and minoxidil [ 130 ] were all successfully formulated and delivered transdermally using transfersomes.…”

Effect of Transfersome Formulation on the Stability and Antioxidant Activity of N-Acetylcysteine in Anti-Aging Cream

“…The entrapment efficiency of transfersome increased with increasing lipid concentration (phospholipid) and decreased with increasing surfactant concentration because lower surfactant concentrations (<15%, b/b) allowed all surfactant molecules to bind to the phospholipid bilayer layer. If the concentration exceeds a certain concentration, some surfactant molecules will cause an increase in the permeability of the vesicle membrane, which can reduce the efficiency of entrapment [15,16]. Phospholipids are the main components that form vesicles.…”

“…Lipid degradation can also occur because of chemical degradation processes that can affect the stability of the phospholipid bilayer, such as hydrolysis and lipid oxidation. Degradation of the lipid bilayer can reduce the absorption efficiency of N-acetylcysteine so that more free N-acetylcysteine is oxidized [15].…”

Formulation and Evaluation of Cream of Green Tea Extract and Salicylic Acid for Acne Treatment

“…The primary catechin in green tea, epigallocatechin-3-gallate (EGCG), has been demonstrated to have ant melanogenesis, moisture retention, and skin-protecting properties. According to recent research EGCG might also help in wound healing [10]. Salicylic acid is a chemical substance that belongs to the class of aromatic hydroxy carboxylic acids (C7H6O3).…”