Effect of Transfersome Formulation on the Stability and Antioxidant Activity of N-Acetylcysteine in Anti-Aging Cream

Abstract

Objective: N-acetylcysteine is an antioxidant with thiol/sulfhydryl groups and is currently being developed as an active ingredient in anti-agingcreams. The study’s aim was to compare the stability and antioxidant activity of N-acetylcysteine in anti-aging creams formulated with and withouta transfersome carrier system.Methods: Stability was assessed by performing cycling, centrifugal, and accelerated stability tests. In addition, antioxidant activity was measuredby the DPPH method, and in vitro penetration was measured using Franz diffusion cells. The analysis of N-acetylcysteine was performed using highperformanceliquid chromatography with ultraviolet–visible detection at a wavelength of 214 nm and a flow rate of 1.0 mL/min, injection volume of5 μL, and a mobile phase of phosphate buffer pH 3.0.Results: The N-acetylcysteine transfersome and non-transfersome cream preparations did not change color or show phase separation during thecycling and centrifugal tests. The N-acetylcysteine in the transfersome and non-transfersome cream preparations had strong antioxidant activity,with half-maximal inhibitory concentrations of 26.90 μg/mL and 38.63 μg/mL, respectively. The in vitro penetration test using Franz diffusion cellsshowed that the cumulative amount of penetrated N-acetylcysteine was 7355.13 μg/cm2 (flux of 845.67 μg/cm2∙h) in the transfersome cream and4677.61 μg/cm2 (flux of 533.33 μg/cm2∙h) in the non-transfersome cream.Conclusion: The in vitro penetration test results showed that the transfersome formulations in creams were able to increase the cumulative amountand flux of penetrated N-acetylcysteine in anti-aging cream preparations relative to those not formulated with transfersome.

Optimization of Cream Formulation Containing Leaf Extract and Chitosan Nanoparticles

“…A cycling test is carried out to test the product against the possibility of crystallisation and as an emulsion test on cream as an indicator of emulsion stability. 28…”

Transfersome as Topical Drug Delivery: Formulation and Characterization

“…Particle size and size distribution results were recorded after each measurement using dynamic light scattering (DLS). Additionally, another instrument that can be used is a particle size analyzer (Harmita, Iskandarsyah, and Afifah 2020).…”

Formulation and Evaluation of Antiaging Ointment Containing Microencapsulated Turmeric and Jojoba Oil

“…The %free radical scavenging activity was carried as per the procedure mentioned in Harmita et al, (2020) 23 and it was get calculated by using the following formula:…”