Formulation of pH responsive multilamellar vesicles for targeted delivery of hydrophilic antibiotics

Niosomes modified with a novel pH-responsive coating (mPEG-OA) enhance the antibacterial and anti-biofilm activity of vancomycin against methicillin-resistant Staphylococcus aureus

“…The biosafety of the mPEG-OA-coated VCM niosomes was assessed using the MTT assay [49]on Henrietta Lack's cervical cancer (HeLa), Michigan Cancer Foundation-7 (MCF-7) breast cancer, and human embryonic kidney 293 (HEK-293) cell lines [50]. Briefly, 2.5 ×10 3 of each cell line were seeded in 100 μl of (EMEM for MCF-7, DMEM for HEK-293, and HeLa cells) in a 96-well plate with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin-fungizone and 1% L-glutamine and incubated in a humid atmosphere containing 5% CO 2 at 37 °C for 24 h. Subsequently, a series of dilutions of mPEG-OA-coated VCM niosomes (20, 40, 60, 80, and 100 μg mL −1 ) was added to each well, and cells were incubated.…”

Quaternary ammonium-tethered hyperbranched polyurea nanoassembly synergized with antibiotics for enhanced antimicrobial efficacy

“…[19][20][21] To overcome the biofilm EPS barrier, various strategies have been developed, including the use of biofilm dispersants or EPS degradation agents for enhanced antibiotic penetration. [22][23][24][25][26][27][28][29] Biofilm dispersants can be classified into two types based on their sites of action. The first type includes enzymes or surfactants that target the EPS, and consists mainly of polysaccharides, extracellular DNA, and proteins.…”

A pH-responsive supramolecular antibacterial agent based on host–guest chemistry

“…[4][5][6] Inspired by the effective application of microenvironmentresponsive nanomaterials in tumor therapy, a variety of antibacterial platforms that respond to the environment at the infection site for anti-infection therapy have been constructed by scientists. [7][8][9][10][11][12][13][14] The microenvironment of the inflammatory infection site is different from that of normal tissues, including elevated contents of hydrolytic enzymes and toxins, elevated local temperatures, lower oxygen contents, and lower pH values. [15][16][17] The acidic infection microenvironment can serve as an ''endogenous switch'' for stimulating responsive drug delivery due to anaerobic glycolysis.…”