Formulation, In Vitro and In Vivo Pharmacokinetics of Anti-HIV Vaginal Bioadhesive Gel

Chatterjee, Arkendu; Bb, Bhowmik; Ys, Thakur

doi:10.4103/0975-1483.80290

Cited by 19 publications

(8 citation statements)

References 11 publications

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“…At predetermined time intervals, 5 mL of the samples was withdrawn from the receiver compartment and replaced by the same volume of freshly prepared PBS (pH 6.8). The withdrawn samples were analyzed by the UV-visible spectrophotometer at 420 nm [21]. …”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract

Nagaich

Gulati

Chauhan

2016

Journal of Pharmaceutics

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The advancement of the biological production of nanoparticles using herbal extracts performs a significant role in nanotechnology discipline as it is green and does not engage harsh chemicals. The objective of the present investigation was to extract flavonoids in the mode of apple extract and synthesize its silver nanoparticles and ultimately nanoparticles loading into hydrogels. The presence of flavonoids in apple extract was characterized by preliminary testing like dil. ammonia test and confirmatory test by magnesium ribbon test. The synthesized silver nanoparticles were characterized using UV spectroscopy, particle size and surface morphology, and zeta potential. Silver nanoparticles loaded hydrogels were evaluated for physical appearance, pH, viscosity, spreadability, porosity, in vitro release, ex vivo permeation, and antibacterial (E. coli and S. aureus) and antioxidant studies (DPPH radical scavenging assay). Well dispersed silver nanoparticles below were observed in scanning electron microscope image. Hydrogels displayed in vitro release of 98.01% ± 0.37% up to 24 h and ex vivo permeation of 98.81 ± 0.24% up to 24 h. Hydrogel effectively inhibited the growth of both microorganism indicating good antibacterial properties. The value of percent radical inhibition was 75.16% ± 0.04 revealing its high antioxidant properties. As an outcome, it can be concluded that antioxidant and antiageing traits of flavonoids in apple extract plus biocidal feature of silver nanoparticles can be synergistically and successfully utilized in the form of hydrogel.

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Section: Methodsmentioning

confidence: 99%

Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract

Nagaich

Gulati

Chauhan

2016

Journal of Pharmaceutics

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“…The weight of 1000 mg for measuring gel strength was allowed to penetrate into the gel. The gel strength, which means the viscosity of the gel, was determined by time (seconds), the weight took to sink 5 cm down through the prepared gel [21].…”

Section: Gel Strength Determinationmentioning

confidence: 99%

Formulation and Evaluation of Metronidazole Microspheres-Loaded Bioadhesive Vaginal Gel

Kalita

Saikia²,

Kalita³

2017

Asian J Pharm Clin Res

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Objectives: The objective of the present work is to develop and characterize metronidazole microsphere-loaded bioadhesive vaginal gel to ensurelonger residence time at the infection site, providing a favorable release profile for the drug.Methods: Microsphere was prepared by solvent evaporation method in various ratios of metronidazole to poly-ε-caprolactone (PCL). Physicochemicalevaluation of microspheres includes determination of solubility in simulated vaginal fluid, partition coefficient (n-octanol/citrate phosphatebuffer pH 4.5), particle size distribution, entrapment efficiency, X-ray diffraction, and surface morphology by scanning electron microscopy (SEM).Drug excipient compatibility was established by Fourier transform infrared and differential scanning calorimetry studies. Bioadhesive gel wasprepared using Carbopol 934P and HPMC K4M in various concentrations, and methyl paraben was used as a preservative. The pH was adjustedwith triethanolamine which resulted in a translucent gel. The optimized metronidazole microsphere formulation was dispersed into the gel base.Microspheres in gel formulations were evaluated for pH, viscosity, spreadability, drug content, and gelling strength. Ex vivo mucoadhesive strength ofthe gel was determined on goat vaginal mucosa. In vitro drug release study was performed using cellophane membrane.Results: The optimized batch of microsphere F4 (drug-polymer ratio 1:4) showed entrapment efficiency of 72.62±3.66%, solubility of 1.5 mg/ml, andpartition coefficient of 0.12. Particle size of all the formulations was observed below 100 μm. Regular and spherical particles were observed in theSEM photomicrographs. The optimized gel formulation G5 (Carbopol and HPMC at 1: 0.25 ratio) showed viscosity of 7538 cps at 100 RPM, gel strengthrecorded as 35 secobds for a 1000.00 mg load, and spreadability of 4.6 g.cm/seconds. G5 showed 82.4% drug release at 10.0 hrs and mucoadhesivestrength of 6.5±1.2 g.Conclusion: The study results suggest that metronidazole-loaded PCL microsphere in mucoadhesive gel would provide a mean for sustainedtreatment of vaginal infections.Keywords: Microsphere, Metronidazole, Bioadhesive vaginal gel.

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“…The cumulative amount of drug released expressed in % was plotted for formulation. 18,19,20 Diffusion cell apparatus of Orchid Scientificus and UV-Visible spectrophotometer (Pharma Spec UV-1700) from Shimadzu, Motic photomicroscope and Bruker's FT-IR spectrophotometer.…”

Section: In-vitro Diffusion Studymentioning

confidence: 99%

Formulation Development and Evaluation of Anti-inflammatory Potential of Cordia obliqua Topical Gel on Animal Model

Gupta¹

2017

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Background:The plants of genus Cordia has been used in traditional medicine for many diseases from history. Cordia obliqua Willd. is also a medicinally important plant and it has been proved by modern scientific research. Objective: This study is aimed to formulate and characterize a topical gel from Cordia obliqua leaf methanol extract and evaluate its effect on carrageenan induced rat paw edema. Materials and Methods: The methanol extract was prepared by successive solvent extraction using soxhlet apparatus. Topical gel was designed to prepare by Dispersion method using Carbopol 940 as polymer. The various gel characteristics was studied by standard procedures like Homogeneity, grittiness, extrudability, drug content, spread ability and in-vitro diffusion study. The anti-inflammatory potential of gel was evaluated by Carrageenan induced rat paw edema method in comparison with standard Diclofenac Sodium gel 1% on Albino rats. Results: The herbal gel was found homogeneous with good extrudability, no grittiness, pH 6.79 and drug content 93.167%. The anti-inflammatory activity of herbal gel was found comparable with standard Diclofenac Sodium gel and has shown 82.71% edema inhibition after 4 h of treatment. Conclusion: Cordia obliqua gel was found suitable as a standard topical gel formulation and it can be used safely for treatment of edema.

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Formulation, In Vitro and In Vivo Pharmacokinetics of Anti-HIV Vaginal Bioadhesive Gel

Cited by 19 publications

References 11 publications

Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract

Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract

Formulation and Evaluation of Metronidazole Microspheres-Loaded Bioadhesive Vaginal Gel

Formulation Development and Evaluation of Anti-inflammatory Potential of Cordia obliqua Topical Gel on Animal Model

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