Formulation and in Vitro, in Vivo Evaluation of Proniosomal Gel of Neomycin Sulphate

Shete, Amol; Thorat, P. V.; Doijad, Rajendra; Sajane, Sachin

doi:10.22159/ijap.2019v11i2.30614

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…At appropriate time intervals, samples were withdrawn and were replaced by equal volumes of fresh receptor fluid on each withdrawal, and samples were analyzed spectrophotometrically at 206 nm [9,18,21,22,28].…”

Section: Ex Vivo Skin Permeation Studiesmentioning

confidence: 99%

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A Novel Vesicular Approach for Transdermal Administration of Enalapril Maleate Loaded Nanoproniosomal Gel: Formulation, Ex Vivo Evaluation and in Vivo Antihypertensive Study

2020

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Objective: The objective of the study was to formulate and evaluate the nanoproniosomal gel of Enalapril maleate (EM) for the treatment of hypertension through the transdermal administration and to provide better bioavailability. Methods: The nanoproniosomal gel of the EM was formulated by Lecithin, Cholesterol, Non-ionic surfactants using the Coacervation-phase separation method. The prepared nanoproniosomal gels were evaluated for pH and viscosity, vesicle size analysis, rate of spontaneity, entrapment efficiency, zeta potential, ex vivo skin permeation studies, skin irritation test, stability studies and in vivo antihypertensive studies. Results: Physical characterization was found to be within acceptable limits. The ex vivo skin permeation studies showed the cumulative permeation of 58.75 % to 89.72 % through the albino rat skin in 24 h for all the formulations, which indicate the zero-order drug permeation with diffusion, non-fickian release. Among all formulations, EMNP7 was selected as best formulation because it showed better characteristics than other formulations in several aspects like physicochemical characterization, ex vivo skin permeation studies, permeation kinetics, and other evaluation parameters. The skin irritation study revealed that there was no irritation after topical application and it was found to be safer to use. The In vivo antihypertensive study revealed that the formulation of EMNP7 was successful to regress the rat blood pressure (BP) to normal values in experimental hypertensive rats. Conclusion: The nanoproniosomal gel is an efficient transdermal therapeutic system for the delivery of EM for the treatment of hypertension. It is suitable for once a day controlled release formulation.

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Section: Ex Vivo Skin Permeation Studiesmentioning

confidence: 99%

“…The Erythema and Edema were to be observed. Finally, the test sites were to be graded as per the visual scoring scale [22,28,[33][34][35].…”

Section: Skin Irritation Studiesmentioning

confidence: 99%

A Novel Vesicular Approach for Transdermal Administration of Enalapril Maleate Loaded Nanoproniosomal Gel: Formulation, Ex Vivo Evaluation and in Vivo Antihypertensive Study

2020

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“…Each proniosomal gel (100 mg) formulation was hydrated in a small vial containing 10 mL of phosphate-buffered saline and shaken manually for 5 min. The resulting niosomes were microscopically examined using an Olympus BX51 Fluorescence Microscope to measure their average vesicle sizes [8,19,20].…”

Section: Vesicle Size Determination (Microscopic Evaluation)mentioning

confidence: 99%

Formulation of Enalapril Maleate Nanoproniosomal Gels and Their Pharmacokinetic Evaluations in Hypertensive Albino Wistar Rats: Ex Vivo and In Vivo Approaches

Sabareesh,

Rajangam,

Yanadaiah

2024

Nano Biomedicine and Engineering

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Proniosomes are drug-encapsulated, nanoscale vesicular structures that generate multilamellar niosomal dispersions upon hydration. This study aimed to develop enalapril maleate nanoproniosomal gels and assess their effectiveness in experimental hypertensive rat models. The gels were synthesized based on the coacervation phase separation method, using lecithin, cholesterol, and various nonionic surfactants as formulation components, along with the drug. The developed gels were then subjected to various analyses, such as pH, viscosity, rate of spontaneity, entrapment efficiency, vesicle size, ex vivo permeation, skin irritation, scanning electron microscopy, stability, in vivo bio-availability, in vivo antihypertensive activity, and in vitro-in vivo correlation studies. Results revealed that all synthesized gel formulations maintained good physical characteristics, within permissible limits. The results of the ex vivo skin permeation analysis revealed non-Fickian release kinetics and zero-order penetration behaviors of the drug formulations with diffusion, achieving a cumulative permeation rate of 58.75%-89.72% through albino rat skin over 24 h. Moreover, skin irritation tests revealed that the topical application of the drug formulations did not cause any signs of irritation, indicating their safety. Furthermore, in vivo bio-availability studies revealed that one particular formulation, EMNP7, demonstrated an approximately 188.99-fold greater bio-availability compared to the Vasotec tablet. Additionally, in vivo antihypertensive analysis revealed that this formulation effectively restored elevated rat blood pressures to the normal range. Furthermore, the in vitro-in vivo correlation analysis suggested that the ex vivo (in vitro) data could accurately replicate in vivo physiological conditions. Overall, our findings indicate that enalapril maleate encapsulated within nanoproniosomal gels can effectively function as controlled drug delivery systems, releasing the drug once per day for effective hypertension management.

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“…They were placed into the rat holder for 5-6 d for a period of 10-20 min. By repeating the exercise, the animals taught to remain in the rat holder calmly and they became acquainted with the experimental conditions [36][37][38][39].…”

Section: Pharmacodynamic Activity Of Ocpgmentioning

confidence: 99%

Proniosomal Gel Mediated Transdermal Delivery of Glibenclamide and Atenolol Combination: Exvivo and Pharmacodynamic Studies

Anitha

Satyanarayana

2021

Int J App Pharm

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Objective: The objective of the present work was to develop an optimized dosage form for treating comorbidity in combination and evaluate it for its pharmacodynamic performance in male Wistar albino rats. Methods: Transdermal proniosomal gel for Combination of Glibenclamide (GLB) and Atenolol (ATN) was developed and optimized by Box Behnken design. This optimized combinational proniosomal gel (OCPG), which was selected by a point prediction method, was evaluated for its ex vivo, skin irritation studies and pharmacodynamic activities of both drugs in rats in comparison with its oral therapy. Results: The ex-vivo permeation behavior through different skins was studied and the findings were also confirmed by the values of the steady-state flux (Jss). The OCPG observed an increase of more than twice in the cumulative amount of impregnated drugs compared to pure drug films. The study on skin irritation revealed the non-irritability of the developed OCPG applied. OCPG significantly showed sustained hypoglycemic activity in rats (p<0.001), when compared to orally treat animals up to 24 h. Systolic blood pressure (SBP) lowering effect of OCPG was found to be significant (p<0.02), when compared to orally treat rats up to 24 h. However, the reduction was slow and sustained in the case of OPCG where a significant response was observed in the performed studies. Conclusion: Overall, the results show that controlled release GLB and ATN proniosomes offer a useful and promising transdermal delivery system. Henceforth this may be an achievement in treating the diabetic hypertensive patient.

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Formulation and in Vitro, in Vivo Evaluation of Proniosomal Gel of Neomycin Sulphate

Cited by 4 publications

References 9 publications

A Novel Vesicular Approach for Transdermal Administration of Enalapril Maleate Loaded Nanoproniosomal Gel: Formulation, Ex Vivo Evaluation and in Vivo Antihypertensive Study

A Novel Vesicular Approach for Transdermal Administration of Enalapril Maleate Loaded Nanoproniosomal Gel: Formulation, Ex Vivo Evaluation and in Vivo Antihypertensive Study

Formulation of Enalapril Maleate Nanoproniosomal Gels and Their Pharmacokinetic Evaluations in Hypertensive Albino Wistar Rats: Ex Vivo and In Vivo Approaches

Proniosomal Gel Mediated Transdermal Delivery of Glibenclamide and Atenolol Combination: Exvivo and Pharmacodynamic Studies

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