Formulasi dan Karakterisasi SNE (Self Nanoemulsion) Astaxanthin dari Haematococcus pluvialis sebagai Super Antioksidan Alami

Abstract

ABSTRACT:Astaxanthin is a group of fat-soluble xanthophyll carotenoid found in many microorganisms and marine animals. The problem that exists in its use as active compound of antioxidants is due to lipophilicity and poor stability in the gastrointestinal tract thereby making low bioavailability. In this research offers the nanotechnology to develop astaxanthin nanoemulsion which is intended to improve the stability in the dosage form and also further developing new delivery paths in the use of antioxidants through transdermal delivery route so it has the optimal deliveries. In this research will be made nanoemulsion astaxanthin formulation to produce good physical and chemical characteristics. Nanoemulsion were prepared by using the self-nanoemulsifying (SNE) method. Optimizations of formula were performed ranging from oil phase screening, surfactant type screening, and optimization of ratio of oil phase : surfactant : co-surfactant. Characterization of nanoemulsion were carried out by physical characterizations including globul size and polydispersity index, zeta potential, visual appearance, and globul morphology. Chemical Characterization included the entrapment efficiency test. The results showed that the nanoemulsion Astaxanthin has a 10-20 nm globul size (with normal size distribution curve), the polydispersity index value is less than 0.5, the zeta potential is greater than (-20) mV, the entrapment efficiency is between 80-87%, and has spherical globules form. ABSTRAK:Astaxanthin merupakan kelompok karotenoid xantofil larut lemak yang banyak ditemukan pada berbagai mikroorganisme dan hewan laut. Permasalahan yang terdapat di dalam pengunaannya sebagai bahan aktif sumber antioksidan adalah karena lipofilisitasnya dan stabilitas astaxanthin yang rendah di dalam saluran cerna sehingga membuat ketersediaan hayati yang rendah. Di dalam penelitian ini menawarkan teknologi nano untuk mengembangkan nanoemulsi astaxanthin yang ditujukan untuk meningkatkan stabilitas astaxanthin di dalam sediaan dan juga kedepannya untuk mengembangkan jalur penghantaran baru dalam pemakaian antioksidan yakni melalui rute transdermal sehingga ditujukan agar penggunaan astaxanthin dapat optimal. Di dalam penelitian ini akan dibuat formulasi nanoemulsi astaxanthin untuk menghasilkan karakteristik fisik dan kimia yang baik. Nanoemulsi dibuat dengan menggunakan metode Nanoemulsi spontan (SNE). Dilakukan optimasi formula mulai dari skrining fase minyak, skrining jenis surfaktan, dan optimasi rasio fase minyak:surfaktan:kosurfaktan. Karakterisasi nanoemulsi berupa karakterisasi secara fisik meliputi ukuran globul dan indeks polidispersitas, potensial zeta, tampilan visual, dan morfologi globul. Karakterisasi secara kimia meliputi uji efisiensi penjeratan. Hasil penelitian menunjukkan nanoemulsi Astaxanthin yang dikembangkan memiliki ukuran globul 10-20 nm (dengan kurva distribusi ukuran globul normal), nilai indeks polidispersitas kurang dari 0.5, potensial zeta lebih besar dari (-20) mV, dan efisiensi penjeratan berkisar antara 80-87% s...

Antidiabetic activity of thin film containing astaxanthin-loaded nanoemulsion using carboxymethylcellulose sodium polymer on alloxan-induced diabetic rabbit

“…In this study, As-NE was prepared by using self-nanoemulsifying method which refers to the previous study. [ 8 ] When astaxanthin is incorporated into the nanoemulsion system, it will dissolve in the oil phase and enters the micelles system, which thermodynamically stable, with the droplet size is <100 nm. [ 10 ] As-NE had droplets size in the nano-range (10–20 nm) with polydispersity index was <0.5 which indicates that the nanoemulsion had uniform size distribution and stable for a long period of time.…”

“…Astaxanthin was loaded into the nanoemulsion using a combination between oil phase, surfactant and co-surfactant that had been optimized in our previous studies. [ 8 ] Thin film preparation was performed using solvent casting method using Carboxymethylcellulose sodium (CMC-Na) polymer as a matrix system. Chemical, physical, and mechanical evaluations of FDT-As-NE were carried out for intraoral purposes.…”

A Preparation, Characterization, and in Vitro Skin Penetration of Morus Alba Root Extract Nanoemulsion

“…Research by Nurdianti et al (2017), nanoemulsion on astaxanthin developed with sunflower oil composition, Kolliphor RH40, and PEG 400 (1:8:1) has a potential zeta value of −5.38 mV [27]. Compared with white mulberry root extract nanoemulsion, the two nanoemulsions have a potential zeta value which is close to 0 because it used a surfactant and non-ionic surfactant.…”

Formulation, Characterization and Stability Study of Fast Dissolving Thin Film Containing Astaxanthin Nanoemulsion Using Hydroxypropylmethyl Cellulose Polymer

“…Film disintegration time was determined visually in a petri dish containing 10 ml of phosphate buffer pH 6.8 at 37 °C where the container was shaken in every 10 s. Disintegration time is the time at which the film begins to break or collapse. The disintegration time of a good thin film is less than 60 s [13].…”