Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells

Wang, Yao; Zhu, Yunxia; Gao, Lu; Yin, Han; Xie, Zuoling; Wang, Dong; Zhu, Zhengqiu; Han, Xiao

doi:10.3390/molecules170910052

Cited by 59 publications

(41 citation statements)

References 44 publications

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“…For example, formononetin reduced the action of IL-1β and nuclear factor κB (NF-κB) in vitro (Wang et al, 2012). Also, the anti-inflammatory and antioxidant activities of formononetin promoted neuron and lung protective effects in vivo, by means of decreasing the levels of TNF-α and IL-6 and…”

Section: Figurementioning

confidence: 99%

Antinociceptive and anti-inflammatory effects of Brazilian red propolis extract and formononetin in rodents

Cavendish

Santos

Neto

et al. 2015

Journal of Ethnopharmacology

121

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Section: Figurementioning

confidence: 99%

Antinociceptive and anti-inflammatory effects of Brazilian red propolis extract and formononetin in rodents

Cavendish

Santos

Neto

et al. 2015

Journal of Ethnopharmacology

121

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“…These isoflavones have been reported for their antidiabetic activity . They regulate the expression of various proteins associated with pathogenesis of diabetes such as PI3 K, MAP kinase, PPAR, NF‐k β , visfatin and SIRT1 …”

Section: Introductionmentioning

confidence: 99%

Trifolium pratense (Red Clover) Improve SIRT1 Expression and Glycogen Content in High Fat Diet‐Streptozotocin Induced Type 2 Diabetes in Rats

Oza

Kulkarni

2020

Chemistry & Biodiversity

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Flowering tops of Trifolium pratense L. (Fabaceae) are known for its traditional medicinal values. In present study, our aim was to investigate effect of standardized aqueous extract of flowering tops of Trifolium pratense L. on insulin resistance and SIRT1 expression in type 2 diabetic rats. Type 2 diabetes was induced by feeding high fat diet and administering low dose of streptozotocin. Diabetic animals were treated with standardized aqueous extract at three different doses. Parameters such as blood glucose, lipid profile, glycohemoglobin, insulin sensitivity, HOMA-IR and liver glycogen content were measured. Changes in morphology and expression of SIRT1 in pancreatic tissue were measured in histopathological and immunohistological studies. Aqueous extract treatment showed reduction in hyperglycemia and improved insulin sensitivity. Extract treatment also showed reduction in formation of glycated hemoglobin and improved liver glycogen level. Histopathological study revealed protecting effect of extract in pancreatic tissue against hyperglycemia induced damage. Treatment increased expression of SIRT1 in rat pancreatic tissue. Results indicate that the aqueous extract of Trifolium pratense had beneficial role in improving insulin sensitivity and SIRT1 expression. Figure 1. A chromatogram of (a) formononetin and biochanin standards and (b) aqueous extract of flowering tops of Trifolium pratense L.

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“…Up to now, all available information regarding the role and regulation of cytokine-mediated NF-κB activation in beta cells has focused on the canonical pathway [2,[16][17][18]. However, crosstalk between the canonical and non-canonical pathways might exist [13,19].…”

Section: Introductionmentioning

confidence: 99%

The non-canonical NF-κB pathway is induced by cytokines in pancreatic beta cells and contributes to cell death and proinflammatory responses in vitro

et al. 2015

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Aims/hypothesis Activation of the transcription factor nuclear factor (NF)-κB by proinflammatory cytokines plays an important role in beta cell demise in type 1 diabetes. Two main signalling pathways are known to activate NF-κB, namely the canonical and the non-canonical pathways. Up to now, studies on the role of NF-κB activation in beta cells have focused on the canonical pathway. The aim of this study was to investigate whether cytokines activate the non-canonical pathway in beta cells, how this pathway is regulated and the consequences of its activation on beta cell fate. Methods NF-κB signalling was analysed by immunoblotting, promoter reporter assays and real-time RT-PCR, after knockdown or overexpression of key genes/proteins. INS-1E cells, FACS-purified rat beta cells and the human beta cell line EndoC-βH1 exposed to cytokines were used as models. Results IL-1β plus IFN-γ induced stabilisation of NF-κB-inducing kinase and increased the expression and cleavage of p100 protein, culminating in the nuclear translocation of p52, the hallmark of the non-canonical signalling. This activation relied on different crosstalks between the canonical and non-canonical pathways, some of which were beta cell specific. Importantly, cytokine-mediated activation of the non-canonical pathway controlled the expression of 'late' NF-κB-dependent genes, regulating both pro-apoptotic and inflammatory responses, which are implicated in beta cell loss in early type 1 diabetes. Conclusions/interpretation The atypical activation of the non-canonical NF-κB pathway by proinflammatory cytokines constitutes a novel 'feed-forward' mechanism that contributes to the particularly pro-apoptotic effect of NF-κB in beta cells.

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Formononetin Attenuates IL-1β-Induced Apoptosis and NF-κB Activation in INS-1 Cells

Cited by 59 publications

References 44 publications

Antinociceptive and anti-inflammatory effects of Brazilian red propolis extract and formononetin in rodents

Antinociceptive and anti-inflammatory effects of Brazilian red propolis extract and formononetin in rodents

Trifolium pratense (Red Clover) Improve SIRT1 Expression and Glycogen Content in High Fat Diet‐Streptozotocin Induced Type 2 Diabetes in Rats

The non-canonical NF-κB pathway is induced by cytokines in pancreatic beta cells and contributes to cell death and proinflammatory responses in vitro

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