Formin-2 is required for spindle migration and for the late steps of cytokinesis in mouse oocytes

Dumont, Julien; Million, Karine; Sunderland, Kelsey; Rassinier, Pascale; Lim, Hyunjung Jade; Leader, Benjamin; Verlhac, Marie‐Hélène

doi:10.1016/j.ydbio.2006.08.044

Cited by 167 publications

(167 citation statements)

References 35 publications

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“…Notably, these studies were conducted on naked oocytes that therefore lacked cumulus cell contact. More recently, the actin binding protein formin-2 has been implicated in the process of GV anchoring to the cortex as deletion of this actin binding protein results in central spindle assembly and failure of the meiosis-1 spindle to migrate back to the cortex [16,24]. Formin-2 deficits are linked directly to gross aneuploidy in embryos that fail to develop normally.…”

Section: Discussionmentioning

confidence: 99%

Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence

Barrett

Albertini

2009

J Assist Reprod Genet

103

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Purpose To investigate the role of cumulus cell contact during oocyte maturation on meiotic spindle assembly and the acquisition of developmental competence. Methods Cumulus oocyte complexes isolated from mouse ovaries subjected to in vitro or in vivo maturation were analyzed by confocal microscopy with respect to oocyte somatic cell contacts and for their ability to develop after parthenogenic activation during embryo culture. Results Cell contact is maintained during maturation in vivo, predisposing oocytes to cortical meiotic spindle assembly and developmental competence acquisition. In contrast, oocytes matured in vitro lose cell contact coincident with central meiotic spindle assembly that results in cleavage delays upon egg activation and failure to form blastocysts. Experimental disruption of cell contact by the actin-depolymerizing agent latrunculin B results in the formation of enlarged meiotic spindles with dispersed chromosomes unlike the compact ordering of chromosomes observed on spindles formed after in vivo maturation, suggesting a link between cell contact and the acquisition of developmental competence. Conclusions Somatic cell contact optimizes oocyte quality during meiotic maturation by regulating the spatial organization and function of the meiotic spindle through actindependent mechanisms that enhance development.

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Section: Discussionmentioning

confidence: 99%

Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence

Barrett

Albertini

2009

J Assist Reprod Genet

103

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“…When MTs are disassembled with a depolymerizing agent, e.g., nocodazole, the chromosomes are still able to migrate toward the cortex, even at higher speed [Longo and Chen, 1985;Van Blerkom and Bell, 1986;Verlhac et al, 2000;Li et al, 2008]. MI spindle migration is an actin cytoskeleton dependent process, as it is blocked by inhibiting actin polymerization Verlhac et al, 2000;Dumont et al, 2007a;Li et al, 2008] or F-actin turn-over [Terada et al, 2000;Li et al, 2008]. The underlying molecular mechanism of spindle migration has started to emerge when an actin nucleating protein, formin-2 was found to be essential for this process.…”

Section: Spindle Migration: Where and How The Force Is Generatedmentioning

confidence: 99%

“…Yi and Li formin-2 is also involved in the late steps of cytokinesis [Dumont et al, 2007a]. Recently, Pfender et al (2011) found that Spire 1 and 2, another type of actin nucleators, cooperate with formin-2 to nucleate actin filaments in mouse oocytes.…”

Section: Spindle Migration: Where and How The Force Is Generatedmentioning

confidence: 99%

Actin cytoskeleton in cell polarity and asymmetric division during mouse oocyte maturation

2012

Cytoskeleton

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Mammalian oocyte maturation involves two successive rounds of extremely asymmetric cell divisions (known as polar body extrusion) to generate a functional haploid egg. Successful polar body extrusion relies on establishment of an asymmetric spindle position and cortical polarity. Decades of studies using mouse oocytes as a model have revealed critical roles for a dynamic actin cytoskeleton in this process. Here, we review the contribution of actin to the critical events during oocyte meiotic cell divisions with an emphasis on recent advances in understanding the underlying molecular and physical mechanisms. V C 2012 Wiley Periodicals, Inc

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“…27 During mouse oocyte meiotic maturation, actin was required for the chromosome migration, 9,28 cortical spindle anchorage, 29,30 cortex development and polarity establishment 2,31 and first polar body extrusion. 6,27 Additionally, some actin nucleation factors such as Arp2/3 complex, 6 Formin2 7,29 and Spire1/2 32 were involved in the actin organization during mouse oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

RhoA-mediated MLC2 regulates actin dynamics for cytokinesis in meiosis

Duan

Zhu

et al. 2016

Cell Cycle

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During oocyte meiosis, the bipolar spindle forms in the central cytoplasm and then migrates to the cortex. Subsequently, the oocyte extrudes the polar body through two successive asymmetric divisions, which are regulated primarily by actin filaments. Myosin light chain2 (MLC2) phosphorylation plays pivotal roles in smooth muscle contraction, stress fiber formation, cell motility and cytokinesis. However, whether MLC2 phosphorylation participates in the oocyte polarization and asymmetric division has not been clarified. The present study investigated the expression and functions of MLC2 during mouse oocyte meiosis. Our result showed that p-MLC2 was localized in the oocyte cortex, with a thickened cap above the chromosomes. Meanwhile, p-MLC2 was also localized in the poles of spindle. Disruption of MLC2 activity by MLC2 knock down (K D ) caused the failure of polar body extrusion. Immunofluorescent staining showed that a large proportion of oocytes arrested in telophase stage and failed to undergo cytokinesis after culturing for 12 hours. In the meantime, actin filament staining at oocyte membrane and cytoplasm were reduced in MLC2 K D oocytes. Finally, we found that the phosphorylation of MLC2 protein levels was decreased after disruption of RhoA activity. Above all, our data indicated that the RhoA-mediated MLC2 regulates the actin organization for cytokinesis during mouse oocyte maturation.

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Formin-2 is required for spindle migration and for the late steps of cytokinesis in mouse oocytes

Cited by 167 publications

References 35 publications

Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence

Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence

Actin cytoskeleton in cell polarity and asymmetric division during mouse oocyte maturation

RhoA-mediated MLC2 regulates actin dynamics for cytokinesis in meiosis

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