RhoA-mediated MLC2 regulates actin dynamics for cytokinesis in meiosis

Duan, Xing; Li, Jun; Zhu, Chengcheng; Wang, Qiao-Chu; Cui, Xiang‐Shun; Kim, Nam‐Hyung; Xiong, Bo; Sun, Shao‐Chen

doi:10.1080/15384101.2015.1128590

Cited by 14 publications

(12 citation statements)

References 34 publications

(40 reference statements)

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“…Cofilin is a key regulator of actin filament dynamics and reorganization by promoting depolymerization and severing actin filaments (20, 41). Regulation of cofilin via ROCK and LIM kinase is shown to be involved in actin regulations during oocyte maturation (17, 19). We observed that the decrease in actin levels induced by FLNA knockdown was restored by overexpressing active cofilin (S3A) mutant.…”

Section: Discussionmentioning

confidence: 99%

Filamin A is required for spindle migration and asymmetric division in mouse oocytes

et al. 2017

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Dynamic changes in the actin network are crucial for the cortical migration of spindles and establishment of polarity, to ensure asymmetric division during meiotic maturation. In this study, filamin A (FLNA) was found to be an essential actin regulator that controlled spindle migration and asymmetric division during oocyte meiosis. FLNA was localized in the cytoplasm and enriched at the cortex and near the chromosomes. Knockdown of FLNA impaired meiotic asymmetric division and spindle migration with a decrease in the amount of cytoplasmic actin mesh and cortical actin levels. Moreover, FLNA knockdown reduced the phosphorylation of cofilin and Rho kinase (ROCK) near the spindle. Similar phenotypes, such as decreased filament actin levels, impaired spindle migration and polar body extrusion, were observed when active cofilin (S3A) was overexpressed or ROCK was inhibited. Notably, we found that FLNA and ROCK interacted directly in mouse oocytes. Taken together, our results show that FLNA plays crucial roles in asymmetric division during meiotic maturation by regulating ROCK-cofilin-mediated actin reorganization.-Wang, H., Guo J., Lin, Z., Namgoong, S., Oh, J. S., Kim, N.-H. Filamin A is required for spindle migration and asymmetric division in mouse oocytes. FASEB J. 31, 3677-3688 (2017

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Section: Discussionmentioning

confidence: 99%

Filamin A is required for spindle migration and asymmetric division in mouse oocytes

et al. 2017

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“…Moreover, TUBA1 increased with oocyte diameter, and CFLN increased with oocyte maturation and cumulus expansion, which supports the necessity of cytokinesis-related transcripts in the final stages of COC maturation. The resulting alterations and abnormalities in oocyte diameter and PB extrusion by RI are due to the direct effect of ROCK on tubulin and other cytoskeleton proteins' organization [31,42,44,[50][51][52][53][54][55][56]. Moreover, ZP thickness decreased with increasing RI supplementation duration.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is important to note that spindle organization and polar body extrusion are not coordinated by a single gene. Many cytokinesis-related proteins, including actin, formin, profilin, cofilin, myosin, and tubulin, are effectors of the ROCK signaling pathway though which spindle positioning is regulated [43,[52][53][54][61][62][63][64]. Moreover, ROCK inhibitor increased cellular microtubule acetylation because ROCK regulates microtubule acetylation via phosphorylation of tubulin polymerization-promoting protein 1 [44,50].…”

Section: Discussionmentioning

confidence: 99%

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

Tukur

Aljumaah

Swelum

et al. 2020

Animals

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This is the first report on a biphasic in vitro maturation (IVM) approach with a meiotic inhibitor to improve dromedary camel IVM. Spontaneous meiotic resumption poses a major setback for in vitro matured oocytes. The overall objective of this study was to improve in vitro maturation of dromedary camel oocytes using ROCK inhibitor (Y-27632) in a biphasic IVM to prevent spontaneous meiotic resumption. In the first experiment, we cultured immature cumulus–oocyte complexes (COCs, n = 375) in a prematuration medium supplemented with ROCK inhibitor (RI) for 2 h, 4 h, 6 h, and 24 h before submission to normal in vitro maturation to complete 28 h. The control was cultured for 28 h in the absence of RI. In the first phase of experiment two, we cultured COCs (n = 480) in the presence or absence (control) of RI for 2 h, 4 h, 6 h, and 24 h, and conducted real-time relative quantitative PCR (qPCR) on selected mRNA transcripts. The same was done in the second phase, but qPCR was done after completion of normal IVM. Assessment of nuclear maturation showed that pre-IVM for 4 h yielded an increase in MII oocyte (54.67% vs. 26.6% of control; p < 0.05). As expected, the same group showed the highest degree (2) of cumulus expansion. In experiment 2, qPCR results showed significantly higher expression of ACTB and BCL2 in the RI group treated for 4 h when compared with the other groups. However, their relative quantification after biphasic IVM did not reveal any significant difference, except for the positive response of BCL2 and BAX/BCL2 ratio after 4 and 6 h biphasic IVM. In conclusion, RI prevents premature oocyte maturation and gave a significantly positive outcome during the 4 h treatment. This finding is a paradigm for future investigation on dromedary camel biphasic IVM and for improving the outcome of IVM in this species.

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“…In mammalian oocytes, actin drove the spindle migration and mediated it moving to the cortex, directly promoting oocyte asymmetric division. 32 In addition, several molecules, such as MLC2, 33 ROCK, 34 and FMNL1, 35 were reported to regulate actin activity for spindle positioning in mouse oocytes. We further investigated whether SKAP2 modulated the spindle migration during oocyte cytokinesis.…”

Section: Discussionmentioning

confidence: 99%

SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes

Liu

et al. 2017

Cell Cycle

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SKAP2 (Src kinase-associated phosphoprotein 2), a substrate of Src family kinases, has been suggested to be involved in actin-mediated cellular processes. However, little is known about its role in mouse oocyte maturation. In this study, we thus investigated the expression, localization, and functions of SKAP2 during mouse oocyte asymmetric division. SKAP2 protein expression was detected at all developmental stages in mouse oocytes. Immunofluorescent staining showed that SKAP2 was mainly distributed at the cortex of the oocytes during maturation. Treatment with cytochalasin B in oocytes confirmed that SKAP2 was co-localized with actin. Depletion of SKAP2 by injection with specific short interfering RNA caused failure of spindle migration, polar body extrusion, and cytokinesis defects. Meanwhile, the staining of actin filaments at the oocyte membrane and in the cytoplasm was significantly reduced after these treatments. SKAP2 depletion also disrupted actin cap and cortical granule-free domain formation, and arrested a large proportion of oocytes at the telophase stage. Moreover, Arp2/3 complex and WAVE2 expression was decreased after the depletion of SKAP2 activity. Our results indicate that SKAP2 regulates the Arp2/3 complex and is essential for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes.

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RhoA-mediated MLC2 regulates actin dynamics for cytokinesis in meiosis

Cited by 14 publications

References 34 publications

Filamin A is required for spindle migration and asymmetric division in mouse oocytes

Filamin A is required for spindle migration and asymmetric division in mouse oocytes

Effects of Short-Term Inhibition of Rho Kinase on Dromedary Camel Oocyte In Vitro Maturation

SKAP2 regulates Arp2/3 complex for actin-mediated asymmetric cytokinesis by interacting with WAVE2 in mouse oocytes

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