Formation of Substrate-Supported Membranes from Mixtures of Long- and Short-Chain Phospholipids

A lipid bilayer was deposited on a 3 μm diameter polystyrene (PS) bead via hydrophobic anchoring of bicelles containing oxyamine-bearing cholesteric moieties reacting with the aldehyde functionalized bead surface. Discoidal bicelles were formed by mixing dimyristoylphosphatidylcholine (DMPC), dihexanoylphosphatidylcholine (DHPC), dimyristoyltrimethylammonium propane (DMTAP), and the oxyamine-terminated cholesterol derivative, cholest-5-en-3β-oxy-oct-3,6-oxa-an-8-oxyamine (CHOLOA), in the molar ratio DMPC/DHCP/DMTAP/CHOLOA (1/0.5/0.01/0.05) in water. Upon exposure to aldehyde-bearing PS beads, a stable single lipid bilayer coating rapidly formed at the bead surface. Fluorescence recovery after photobleaching demonstrated that the deposited lipids fused into an encapsulating lipid bilayer. Electrospray ionization mass spectrometry showed that the short chain lipid DHPC was entirely absent from the PS adherent lipid coating. Fluorescence quenching measurements proved that the coating was a single lipid bilayer. The bicelle coating method is thus simple and robust, can be modified to include membrane-associated species, and can be adapted to coat any number of different surfaces.

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“…In Fig. 4A 30 in their studies of POPC/DHPC bicelles coating glass surfaces also suggested that DHPC was absent from the deposited bilayer.…”

Section: Electrospray Ionization Mass Spectrometry (Esi-ms)mentioning

confidence: 82%

“…[28][29][30] Bicelles, or bilayered micelles, are biomimetic model membranes consisting of mixtures of long-chain and short-chain amphiphiles. The long-chain amphiphiles assemble into a planar lipid bilayer stabilized at its edges by the short-chain amphiphiles.…”

mentioning

confidence: 99%

Single Lipid Bilayer Deposition on Polymer Surfaces Using Bicelles

et al. 2015

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“…Photopolymerizations of (meth)acryl and dienoyl bearing zwitterions must be subsumed under photo-initiated radical polymerization. Yet, the topochemical polymerization of conjugated diynes ("diacetylenes") producing strongly colored polymers is unique (Scheme 9), and has been continuously used for stabilizing lipid model membranes [30][31][32][359][360][361][362][363][364][365][366][367][368][369]. Scheme 9.…”

Section: Synthesis By Chain Growth Polymerizationsmentioning

confidence: 99%

Structures and Synthesis of Zwitterionic Polymers

2014

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Abstract:The structures and synthesis of polyzwitterions ("polybetaines") are reviewed, emphasizing the literature of the past decade. Particular attention is given to the general challenges faced, and to successful strategies to obtain polymers with a true balance of permanent cationic and anionic groups, thus resulting in an overall zero charge. Also, the progress due to applying new methodologies from general polymer synthesis, such as controlled polymerization methods or the use of "click" chemical reactions is presented. Furthermore, the emerging topic of responsive ("smart") polyzwitterions is addressed. The considerations and critical discussions are illustrated by typical examples.

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“…Partial disruption of liposomes with detergent has also been successfully used to facilitate vesicle rupture. Detergents have been used previously to assist vesicle fusion to solid [21] or polymer surfaces [22] .…”

Section: Additional Informationmentioning

confidence: 99%