Formation of Ribonucleosides From Bases by Nucleoside Producing Mutants of Bagillus Subtilis

Studies on the Production of Nucleosides by Microorganisms

“…As previously communicated, the authors observed that inosine-producing mutants of Bacillus subtilise have an extremely strong activity to convert exogenously added hypoxanthine to inosine when glucose is present as a source of carbon (11).…”

Studies on the Production of Nucleosides by Microorganisms

“…Inosine-producing mutants, in de novo pathway, of Bacillus subtilis were previously reported to be able to synthesize inosine from either hypoxanthine or other usual purine bases in high yields (1,2). During the course of work on the microbial production of ribonucleosides, it was observed that some unusual purine bases were also converted into their corresponding ribonucleosides in nearly the same yields as from hypoxanthine to inosine by these mutants.…”

Studies on Inosine Fermentation-Production of Inosine by Mutants of Bacillus Subtilis

“…pH was adjusted to 7.0 with KOH followed by autoclaving at 110° for 5 min. KH2P04 was not added, because inosine formation in the cell suspension was fairly repressed by this salt (8,9), and further it was observed that, after 30 hr of the growing culture, phosphate ion in the medium was measured less than 5 mg% as KH2P04. After being cultured for about 30 hr in growing culture medium, at which time the cell growth had reached nearly the stationary phase and inosine was increasing linearly with progress of culture time, CaC03 was separated by centrifugation at 800 rpm for 1 min, and then the cells were harvested by centrifugation at 10,000 rpm for 10 min.…”