Studies on the Production of Nucleosides by Microorganisms

Yamanoi, Akio; Hirose, Yoshiteru; Shiro, Teruo

doi:10.2323/jgam.12.299

Cited by 3 publications

(3 citation statements)

References 3 publications

(7 reference statements)

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“…However, as recognized in the experiment with unusual purines (19), bases are, in general, actively metabolized by these mutants such as converted to ribonucleosides in high yields, but the ribonucleosides are relatively inert. This difference might be related to the different activity between AICA and AICA-R observed in these mutants.…”

Section: Discussionmentioning

confidence: 99%

Studies on Inosine Fermentation-Production of Inosine by Mutants of Bacillus Subtilis

Yamanoi

Konishi

Shiro

1967

J. Gen. Appl. Microbiol.

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Inosine formation by mutants of Bacillus subtilis in chemically defined medium was investigated, using strains No. 11413 (adenine-histidinerequiring) and No. 11023-4 (adenine-requiring).Only adenine (or its congeners) was able to regulate the formation of inosine in the growing culture, and it was also found that adenine was a specific requirement for inhibition of Inosine formation by nongrowing washed cells of these mutants.Purines other than adenine, pyrimidines, and amino acids were all inactive in inhibitory effect on inosine formation by these washed cells. However, the optimum concentration of adenine for the nucleoside formation in growing culture was greatly altered by certain substances. AICA and amino acid mixture (artificial L-amino acid mixture of " casamino acids" type) were found to have a strong adenine-sparing action, while AICA-R, L-histidine, purines, etc., had no such action. AICA inhibited adenine deamination reaction of the intact cells in some cases, which might be one of the mechanisms of the sparing action of the amine. On the other hand, effect of the amino acid mixture on the deamination reaction was not clear under the conditions examined. Another significant effect of the amino acid mixture was its promotive action on the cell growth accompanied with a marked increase in the amount of inosine produced. AICA and some purine compounds were slightly stimulative, but far less than the amino acid mixture in the stimulation of the production.Recently many reports have been presented concerning the microbial production of nucleic acid-related compounds. Inosine was found to be accumulated 1 Abbreviations used in this text are as follows: AICA=5-amino-4-imidazolecarboxamide, AICA-R= AICA-riboside, AICA-RP= AICA-R 5'-monophosphate, 5'-IMP= inosine 5'-monophosphate. 365

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Section: Discussionmentioning

confidence: 99%

Studies on Inosine Fermentation-Production of Inosine by Mutants of Bacillus Subtilis

Yamanoi

Konishi

Shiro

1967

J. Gen. Appl. Microbiol.

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“…These reactions were catalyzed by purine phosphoribosyltransferases, including adenine phosphoribosyltransferase ( apt ), hypoxanthine–guanine phosphoribosyltransferase ( hprT ), and xanthine phosphoribosyltransferase ( xpt ) Bacillus subtilis and other riboflavin producers are also suitable for nucleoside production, and early reports indicated that salvage pathways can be adopted for nucleoside biosynthesis . Moreover, B.…”

Section: Introductionmentioning

confidence: 99%

“…18 Bacillus subtilis and other riboflavin producers are also suitable for nucleoside production, and early reports indicated that salvage pathways can be adopted for nucleoside biosynthesis. 19 Moreover, B. subtilis can also produce nucleosides via the dephosphorylation of nucleotides, and in recent reports, enzymes with corresponding activities have been identified. 20 YktC had 5′-nucleotidase activity, where guanosine 5′monophosphate (GMP) and inosine 5′-monophosphate (IMP) are the preferential substrates, while YcsE exhibited strong 5′-nucleotidase activity with a wider specificity.…”

Section: ■ Introductionmentioning

confidence: 99%

Manipulation of Purine Metabolic Networks for Riboflavin Production in Bacillus subtilis

et al. 2020

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Guanosine monophosphate, the precursor for riboflavin biosynthesis, can be converted to or generated from other purine compounds in purine metabolic networks. In this study, genes in these networks were manipulated in a riboflavin producer, Bacillus subtilis R, to test their contribution to riboflavin biosynthesis. Knocking out adenine phosphoribosyltransferase (apt), xanthine phosphoribosyltransferase (xpt), and adenine deaminase (adeC) increased the riboflavin production by 14.02, 6.78, and 41.50%, respectively, while other deletions in the salvage pathway, interconversion pathway, and nucleoside decomposition genes have no positive effects. The enhancement of riboflavin production in apt and adeC deletion mutants is dependent on the purine biosynthesis regulator PurR. Repression of ribonucleotide reductases (RNRs) led to a 13.12% increase of riboflavin production, which also increased in two RNR regulator mutants PerR and NrdR by 37.52 and 8.09%, respectively. The generation of deoxyribonucleoside competed for precursors with riboflavin biosynthesis, while other pathways do not contribute to the supply of precursors; nevertheless, they have regulatory effects.

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The Microbial Production of Nucleic Acid-Related Compounds

Ogata

1975

Advances in Applied Microbiology Volume 19

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Studies on the Production of Nucleosides by Microorganisms

Cited by 3 publications

References 3 publications

Studies on Inosine Fermentation-Production of Inosine by Mutants of Bacillus Subtilis

Studies on Inosine Fermentation-Production of Inosine by Mutants of Bacillus Subtilis

Manipulation of Purine Metabolic Networks for Riboflavin Production in Bacillus subtilis

The Microbial Production of Nucleic Acid-Related Compounds

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