Formation of multiple treponemes

Wolf, Violetta; Wecke, Jörg

doi:10.1016/s0934-8840(11)80590-6

Cited by 4 publications

(5 citation statements)

References 11 publications

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“…2b). These forms were primarily found in older cultures of DD isolates (Walker et al, 1995) and other treponeme species (Walker et al, 1995;Wecke et al, 1995;Wolf et al, 1993;Wolf & Wecke, 1994). However, the mechanisms leading to circular forms and their possible biological role are still unknown.…”

Section: Treponema Succinifaciens Treponema Socranskii Treponema Amylmentioning

confidence: 99%

Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis

Schrank

Choi

Grund

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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A novel Treponema species was isolated from an ulcerative lesion of a cow suffering from digital dermatitis (DD), a disease which causes painful ulcerations along the coronary band. Among other anaerobic bacteria, high numbers of spirochaetes have been regularly found in DD lesions. Here data are presented of a spirochaete isolated from a DD ulcer. By chemotaxonomy, protein analysis and comparative 165 rDNA sequence analysis this isolate was classified as a treponeme that differed from all Treponema species described previously. The only isolate, DD5/3l, for which the name Treponema brennaborense is proposed, is designated the type strain of the novel species.The strain is a small, highly motile spirochaete that has two periplasmic flagella, one flagellum being attached at each cell pole. Strain DD5/3f exhibits a-glucosidase and N-acetyl-p-glucosaminidase activity and growth is inhibited by rabbit serum. T. brennaborense was phylogenetically most closely related (89.5 O/ O 165 rRNA similarity) to Treponema maltophilum, an oral spirochaete isolated from a periodontitis patient.

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Section: Treponema Succinifaciens Treponema Socranskii Treponema Amylmentioning

confidence: 99%

Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis

Schrank

Choi

Grund

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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“…Recently, Wolf et al reconstructed a model for the morphogenetic process of spherical body (also called multicellular body) formation using different electron microscope techniques including negative‐staining, thin‐sectioning, carbon replication, scanning and freeze‐fracturing of specimens ( 8, 9). During this process, the treponemes lose their individual outer sheath as well as their individual periplasmic flagella and become enclosed by a common outer sheath with a unit or biological membrane profile ( 8, 9). To our knowledge, none of these reports specified any specific environmental and/or nutritional factors responsible for promoting the formation of spherical bodies.…”

Section: Deletion Of Specific Components From Nos Mediummentioning

confidence: 99%

“…For instance, Bladen & Hampp described a common outer sheath surrounding a spherical body ( 1). Recently, Wolf et al reconstructed a model for the morphogenetic process of spherical body (also called multicellular body) formation using different electron microscope techniques including negative‐staining, thin‐sectioning, carbon replication, scanning and freeze‐fracturing of specimens ( 8, 9). During this process, the treponemes lose their individual outer sheath as well as their individual periplasmic flagella and become enclosed by a common outer sheath with a unit or biological membrane profile ( 8, 9).…”

Section: Deletion Of Specific Components From Nos Mediummentioning

confidence: 99%

Factors affecting the formation of spherical bodies in the spirochete Treponema denticola

Ciccio¹,

McLaughlin²,

Chan³

1999

Oral Microbiology and Immunology

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The oral spirochete Treponema denticola typically is a helically shaped, motile bacterial cell. However, morphological variations of T. denticola cells in the form of "spherical bodies" are sometimes seen. Little is known about the environmental factors that cause their formation. The effects of oxygen, growth temperature, nutrient depletion and the addition of metabolic end-products were tested to determine their role in the morphogenesis of the spherical bodies. It was found that the age of the culture, the omission of individual components (yeast extract, rabbit serum, volatile fatty acids or thiamine pyrophosphate) from the medium and the addition of the metabolic end product lactic acid enhanced the formation of these bodies. However, their formation was decreased upon omission of the medium components asparagine and sodium bicarbonate.

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“…The vesicle‐like structures revealed a very low electron density and relatively large dimensions compared to the observed spirochetes. These findings point against the probability that these vesicle‐like structures could be spherical bodies of treponemes which have been described recently [37–39].…”

Section: Discussionmentioning

confidence: 75%

Bacterial adhesion to sulcular epithelium in periodontitis

Vitkov

Hannig

Krautgartner

et al. 2002

FEMS Microbiology Letters

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The purpose of this study was to investigate, by electron microscopy, the type of bacterial attachment to the sulcular epithelium in periodontitis. Gingiva biopsies were observed in a transmission electron microscope using cytochemical staining with ruthenium red for glycocalyx visualisation. In addition, subgingival plaque samples and biopsies from the sulcular epithelium in periodontitis from the patients were estimated microbiologically. Aerobic bacteria only were estimated in the subgingival plaque and both aerobic and anaerobic bacteria in the gingival biopsies. No bacterial internalisation could be observed. Fimbria-mediated adhesion as the only type of bacterial attachment and a large diversity of bacterial glycocalyces were detected. As the fimbrial adhesins of putative periodontal pathogens are able in vitro to induce inflammation and bone resorption via stimulation of the proinflammatory cytokine production, the demonstrated fimbrial adhesins suggest the significant role of bacterial adhesion to sulcular epithelium in periodontitis.

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Formation of multiple treponemes

Cited by 4 publications

References 11 publications

Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis

Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis

Factors affecting the formation of spherical bodies in the spirochete Treponema denticola

Bacterial adhesion to sulcular epithelium in periodontitis

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