Formation of Monodisperse Hierarchical Lipid Particles Utilizing Microfluidic Droplets in a Nonequilibrium State

Mizuno, Masahiro; Toyota, Taro; Konishi, Misako; Kageyama, Yoshiyuki; Yamada, Masao; Seki, Minoru

doi:10.1021/acs.langmuir.5b00043

Cited by 21 publications

(14 citation statements)

References 40 publications

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“…For example, it was shown that using a better water-miscible compound like ethyl acetate (solubility: 64–80 gl −1 (ref. 37 )) produces multilamellar thick-shelled lipid particles 38 . This result is similar to the one we obtained using 2-butanol as the LO phase (solubility: 181 gl −1 (ref.…”

Section: Discussionmentioning

confidence: 99%

Octanol-assisted liposome assembly on chip

et al. 2016

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Liposomes are versatile supramolecular assemblies widely used in basic and applied sciences. Here we present a novel microfluidics-based method, octanol-assisted liposome assembly (OLA), to form monodisperse, cell-sized (5–20 μm), unilamellar liposomes with excellent encapsulation efficiency. Akin to bubble blowing, an inner aqueous phase and a surrounding lipid-carrying 1-octanol phase is pinched off by outer fluid streams. Such hydrodynamic flow focusing results in double-emulsion droplets that spontaneously develop a side-connected 1-octanol pocket. Owing to interfacial energy minimization, the pocket splits off to yield fully assembled solvent-free liposomes within minutes. This solves the long-standing fundamental problem of prolonged presence of residual oil in the liposome bilayer. We demonstrate the unilamellarity of liposomes with functional α-haemolysin protein pores in the membrane and validate the biocompatibility by inner leaflet localization of bacterial divisome proteins (FtsZ and ZipA). OLA offers a versatile platform for future analytical tools, delivery systems, nanoreactors and synthetic cells.

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Section: Discussionmentioning

confidence: 99%

Octanol-assisted liposome assembly on chip

et al. 2016

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“…The principle of LNP production by microfluidic platforms is similar to some of the conventional methods such as hydration and organic solvent injection. Utilization of fluid dynamics mixing, the interface of the microfluidic devices, and the shape of the microfluidic channels effectively assisted the production of the LNPs with desirable properties. ,, Kitazoe et al designed a microfluidic device with one inlet and 13 outlets to produce LNPs encapsulating pDNA. In this study, lipid films blended with cationic polymer (Polylysine) were coated on the inner surface of the glass channels before injecting pDNA to hydrate the lipid film.…”

Section: Applications Of Microfluidic Techniques In Drug Deliverymentioning

confidence: 99%

Recent Advances in Microfluidics for the Preparation of Drug and Gene Delivery Systems

Tomeh

Zhao

2020

Mol. Pharmaceutics

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Drug delivery systems (DDSs) have great potential for improving the treatment of several diseases, especially microbial infections and cancers. However, the formulation procedures of DDSs remain challenging, especially at the nanoscale. Reducing batch-to-batch variation and enhancing production rate are some of the essential requirements for accelerating the translation of DDSs from a small scale to an industrial level. Microfluidic technologies have emerged as an alternative to the conventional bench methods to address these issues. By providing precise control over the fluid flows and rapid mixing, microfluidic systems can be used to fabricate and engineer different types of DDSs with specific properties for efficient delivery of a wide range of drugs and genetic materials. This review discusses the principles of controlled rapid mixing that have been employed in different microfluidic strategies for producing DDSs. Moreover, the impact of the microfluidic device design and parameters on the type and properties of DDS formulations was assessed, and recent applications in drug and gene delivery were also considered.

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“…A similar technique to produce small alginate particles was also reported by Pittermannova et al (2016), wherein 1-undecanol with a surfactant was used as the continuous phase. The usage of this non-equilibrium droplet system has been applied to the production of other types of microparticles, including polymeric (Ono et al, 2014), lipid (Mizuno et al, 2015), protein Yajima et al, 2017), and carbon nanotube particles (Tomii et al, 2017), demonstrating its versatility and usefulness.…”

Section: Particle Production Using Miscible Water-oil Two Phase Owsmentioning

confidence: 99%

Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

Yamada

Seki

2018

Journal of Chemical Engineering of Japan

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With the recent developments in micro uidic instruments and devices, various types of micrometer-sized materials have been produced by employing multiphase ow patterns formed in the microchannel. In particular, microparticles and micro bers, which are compatible with biomolecule incorporation or living cell encapsulations, have been gaining signi cant attention as new tools for biochemical analysis, cellular physiological studies, tissue engineering, cell transplantation, and controlled drug delivery. Herein, we introduce recent developments in micro uidic systems to produce alginate-based hydrogel microparticles and micro bers. By utilizing droplet dispersions either in equilibrium or nonequilibrium states, or by employing parallel laminar ows, microengineered functional materials that are di cult to generate using conventional devices and operations can be obtained. New and interesting multiphase phenomena are reviewed, together with the pros and cons of these systems and their applications. Furthermore, the fundamentals of multiphase micro uidics and the materials used to prepare particles and bers are brie y introduced.

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Formation of Monodisperse Hierarchical Lipid Particles Utilizing Microfluidic Droplets in a Nonequilibrium State

Cited by 21 publications

References 40 publications

Octanol-assisted liposome assembly on chip

Octanol-assisted liposome assembly on chip

Recent Advances in Microfluidics for the Preparation of Drug and Gene Delivery Systems

Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

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