Formation of Malonaldehyde from Phospholipid Arachidonate during Microsomal Lipid Peroxidation

Niehaus, Walter G.; Samuelsson, Bo E.

doi:10.1111/j.1432-1033.1968.tb00428.x

Cited by 1,216 publications

(610 citation statements)

References 16 publications

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“…Serum lipid peroxide was estimated spectrophotometrically using thiobarbituric acid 18 and results were expressed as nM/ml. Carbonylation of serum proteins was measured by modified Levine's method 19 and values were expressed as nM/ml protein.…”

Section: Methodsmentioning

confidence: 99%

A comparative study of serum protein-bound sialic acid in benign and malignant prostatic growth: possible role of oxidative stress in sialic acid homeostasis

Goswami

Nandeesha

Koner

et al. 2007

Prostate Cancer Prostatic Dis

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Benign and malignant prostatic growths are associated with an increase in sialoconjugates (e.g. prostate-specific antigen (PSA)) in blood. Oxidative stress plays a crucial role in pathogenesis of various malignancies. The objective of this study was to evaluate oxidative stress parameters and protein-bound sialic acid level in sera of prostatic tumor cases and to asses for any association between them. Sera samples were collected and estimated for carbonylation of proteins, lipid peroxidation products, PSA and protein-bound sialic acid from 10 patients in each group with prostatic carcinoma (Ca prostate) and benign prostatic hyperplasia (BPH) along with 10 healthy male subjects of similar age group as control. In carcinoma prostate cases, lipid peroxides, protein carbonyls, protein-bound sialic acid and PSA were significantly increased compared to BPH and controls. There was significant association between oxidative stress parameters (lipid peroxide and protein carbonyl) and sialoconjugates (PSA and protein-bound sialic acid). In BPH cases, serum lipid peroxides and protein-bound sialic acid were significantly higher in comparison to controls and protein carbonyls were correlated with protein-bound sialic acid. ROC curve for sialic acid showed that it can be used as a marker to differentiate carcinoma prostate from benign growth of prostate at a cutoff level of 11.38 lg/mg protein with a sensitivity of 100% and specificity of 80%. We conclude that oxidative stress might be associated with the degree of sialylation of protein and graded changes in these parameters possibly unveil the pathogenic demarcation from benign to malignant condition of prostate.

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Section: Methodsmentioning

confidence: 99%

A comparative study of serum protein-bound sialic acid in benign and malignant prostatic growth: possible role of oxidative stress in sialic acid homeostasis

Goswami

Nandeesha

Koner

et al. 2007

Prostate Cancer Prostatic Dis

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“…The activities of non-enzymatic antioxidant such as reduced glutathione (GSH) and enzymatic antioxidants such as reduced glutathione peroxidase (GPx), SOD, CAT and thiobarbituric acid reactive substances (TBARS) were assayed according to the methods of Ellman (1959), Rotruck et al(1973), Kakkar et al(1984), Sinha (1972) and Niehans and Samuelsson (1968), respectively. The protein content in the tissue homogenate was estimated by following the protocol of Lowry et al(1951).…”

Section: Antioxidant Assaymentioning

confidence: 99%

Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties

Balamurugan

Parthasarathi

Ranganathan³

et al. 2008

J. Zhejiang Univ. Sci. B

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Abstract:The hepatoprotective potential of earthworm extract (EE) (Lampito mauritii, Kinberg) was evaluated against paracetamol-induced liver injury in Wistar albino rat, in comparison with silymarin, the standard hepatoprotective drug. We observed a reduction in liver antioxidants, such as glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) and in serum total protein, and an increase in serum alkaline phosphatase (ALP), serum aspertate aminotranferase (AST), serum alanine aminotranferase (ALT), bilirubin and liver thiobarbituric acid reactive substances (TBARS) due to liver injury in the paracetamol-administered rats (2 g/kg). On the contrary, increased activities of liver GSH, SOD, GPx, CAT and serum total protein level, and decrease in the contents of serum ALP, AST, ALT, bilirubin and liver TBARS were observed in rats administered with different doses of EE (100, 200 and 300 mg/kg), which are similar to the activities of hepatoprotective drug silymarin (150 mg/kg). The mode of action of EE as evidenced by the above parameters may suggest that EE, on the one hand, prevents the formation of the reactive oxygen groups, or scavenges these groups, thereby preventing the damage on the hepatic cells, and, on the other hand, modulates the genes responsible for synthesis of antioxidant enzymes such as GPx, CAT and SOD in liver tissue and decreases the serum enzymatic activities such as ALP, AST and ALT.

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“…C [21], Vit. E [22] and lipid per oxidation in terms of TBARS [23], as the case may be in serum, liver, heart and kidney and tri acyl glycerol (TAG) in adipose tissue by standard methods. Histopathology of liver tissues was carried out as per the procedures followed before [9].…”

Section: Methodsmentioning

confidence: 99%

Garlic Oil and Vitamin E Prevent the Adverse Effects of Lead Acetate and Ethanol Separately as well as in Combination in the Drinking Water of Rats

Sajitha

Jose

Andrews

et al. 2010

Indian J Clin Biochem

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Daily feeding of drinking water containing lead acetate (160 mg/l) or 10% alcohol by volume or a combination of both to rats for a month produced certain deleterious effects through oxidative stress. Both heavy metal lead and alcohol are capable of doing such damages. The deleterious alterations observed were in the parameters of blood, serum and tissues, viz; Hb, Pb, proteins, lipids, lipid per oxidation, Vitamins C and E levels and enzyme activities of AST, ALT, and catalase. Simultaneous feeding of either of the two antioxidants garlic oil (GO) and vitamin E at equal doses of 100 mg/kg/day, to the rats counteracted the deleterious effects of the above two chemicals significantly. The maximum damage was brought about by feeding of drinking water containing both lead acetate and alcohol. The protective effects of GO and Vitamin E were not significantly different. The mechanism of actions of the Vitamin E and GO is probably due to their efficiency as detoxifying agents and antioxidants, to scavenging free radicals as well as an independent action of GO on the removal of lead salt as lead sulfide.

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Formation of Malonaldehyde from Phospholipid Arachidonate during Microsomal Lipid Peroxidation

Cited by 1,216 publications

References 16 publications

A comparative study of serum protein-bound sialic acid in benign and malignant prostatic growth: possible role of oxidative stress in sialic acid homeostasis

A comparative study of serum protein-bound sialic acid in benign and malignant prostatic growth: possible role of oxidative stress in sialic acid homeostasis

Hypothetical mode of action of earthworm extract with hepatoprotective and antioxidant properties

Garlic Oil and Vitamin E Prevent the Adverse Effects of Lead Acetate and Ethanol Separately as well as in Combination in the Drinking Water of Rats

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