Formation of glucoraphanin by chemoselective oxidation of natural glucoerucin: A chemoenzymatic route to sulforaphane

Iori, Renato; Bernardi, R.; Gueyrard, David; Rollin, Patrick; Palmieri, Sandro

doi:10.1016/s0960-894x(99)00136-5

Cited by 74 publications

(45 citation statements)

References 4 publications

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“…Since the formation of sulforaphane was quantitative, as confirmed by GC-MS techniques [12], we refer to sulforaphane in the text. Glucoraphanin was obtained by a recently developed procedure [13] whereby a quantitative yield is produced starting from glucoerucin. Glucoerucin was isolated with a high purity grade from the ripe seeds of rocket (Eruca sativa Miller), according to a well-defined protocol [14].…”

Section: Production Of Sulforaphanementioning

confidence: 99%

Cyclin D3 and p53 mediate sulforaphane-induced cell cycle delay and apoptosis in non-transformed human T lymphocytes

Fimognari¹,

Nüsse²,

Berti³

et al. 2002

CMLS, Cell. Mol. Life Sci.

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Despite experimental evidence that sulforaphane can exert chemopreventive effects, whether these effects are specific for neoplastic cells is not known. Following our previous demonstration that sulforaphane induces cell cycle arrest and apoptosis in human T lymphoblastoid Jurkat leukemia cells and increases p53 and bax protein expression, we tested sulforaphane on non-transformed phytohemagglutinin-stimulated human lymphocytes. Here, we demonstrate that sulforaphane arrested cell cycle progression in G, phase, through a decrease in the protein expression of cyclin D3. Moreover, sulforaphane induced apoptosis (and also necrosis), mediated by an increase in the expression of p53. These findings suggest that sulforaphane is a growth modulator for T cells. Our in vitro evidence that sulforaphane is active and even cytotoxic in normal as well as transformed lymphocytes raises important questions regarding its suitability for cancer chemoprevention.

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Section: Production Of Sulforaphanementioning

confidence: 99%

Cyclin D3 and p53 mediate sulforaphane-induced cell cycle delay and apoptosis in non-transformed human T lymphocytes

Fimognari¹,

Nüsse²,

Berti³

et al. 2002

CMLS, Cell. Mol. Life Sci.

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“…E. sativa contains glucosinolates. The hydrolysates of glucosinolates, isothiocyanates, have been reported to inhibit mitosis (Iori et al 1999) and stimulate apoptosis in human tumor cells (Johnson 2002).…”

Section: Introductionmentioning

confidence: 99%

Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall.

Xue

Luo

et al. 2007

Plant Cell Tiss Organ Cult

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To explore the possibility of an effectively long-term preservation of the germplasm of the HR lines of medicinal plant Astragalus membranaceus, Gentiana macrophylla Pall., and Eruca sativa Mill., both cold storage and cryopreservation approaches were attempted and compared. After 5-month cold storage on half strength Murashige and Skoog (1962) (1/2 MS) agar medium (AM), up to 82.9, 75.7, and 100% of the A. membranaceus, G. macrophylla and E. sativa hairy roots (HRs) recovered growth, respectively. The survival rates of A. membranaceus and G. macrophylla HRs significantly decreased, whereas that of E. sativa HR was unchanged with the addition of increased levels of exogenous abscisic acid (ABA) during cold storage. Using the encapsulation-vitrification (EV) method for cryopreservation, the G. macrophylla HRs died, whereas up to 6 and 73% of the A. membranaceus and E. sativa HRs survived, respectively. The HR lines evaluated with both methods showed no significant differences in morphology and growth rate compared with controls that were not subjected to preservation methods. These results suggest that cold storage is a more suitable alternative for the HR lines of the three studied plant species and that specificity of plant species have profound effects on the effectiveness of preservation.

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“…Sulforaphane, the principal component of ESS oil, was found to be a potent inducer of phase II detoxicating enzymes due to its antioxidant functions [6]. Glucoraphanin, that possesses potential antioxidant properties, was obtained from glucoerucin isolated from ripe seeds of Eruca sativa ESS [7]. El-Missiry/El Gindy Alloxan-induced DNA fragmentation in pancreatic islets and cell damage have been attributed to the production of toxic free radicals [8].…”

Section: Introductionmentioning

confidence: 99%

Amelioration of Alloxan Induced Diabetes mellitus and Oxidative Stress in Rats by Oil of <i>Eruca sativa</i> Seeds

El-Missiry¹,

Gindy

2000

Ann Nutr Metab

121

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Clinical research has confirmed the efficacy of several plant extracts in the modulation of oxidative stress associated with diabetes mellitus (DM). Oil of Eruca sativa seeds (ESS) is tried for prevention and treatment of DM induced experimentally by alloxan injection. A single dose of alloxan (100 mg/kg) produced a decrease in insulin level, hyperglycemia, elevated total lipids, triglycerides and cholesterol, decreased high-density lipoprotein and hepatic glycogen contents and elevated hepatic glucose-6-phosphatase activity. Concurrent with these changes, there was an increase in the concentration of malondialdehyde and 4-hydroxynonenal in the liver. This oxidative stress was related to a decreased glutathione (GSH) content and superoxide dismutase activity in the liver of alloxan-diabetic rats. ESS oil (0.06 ml/kg) on its own increased significantly hepatic GSH. Daily oral administration of ESS oil 2 weeks before or after diabetes induction ameliorated hyperglycemia, improved lipid profile, blunted the increase in malondialdehyde and 4-hydroxynonenal and stimulated the GSH production in the liver of alloxan-treated rats. We suggested that ESS oil could be used as antidiabetic complement in case of DM. This may be related to its antioxidative properties and to the increase in hepatic GSH.

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Formation of glucoraphanin by chemoselective oxidation of natural glucoerucin: A chemoenzymatic route to sulforaphane

Cited by 74 publications

References 4 publications

Cyclin D3 and p53 mediate sulforaphane-induced cell cycle delay and apoptosis in non-transformed human T lymphocytes

Cyclin D3 and p53 mediate sulforaphane-induced cell cycle delay and apoptosis in non-transformed human T lymphocytes

Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill., Astragalus membranaceus and Gentiana macrophylla Pall.

Amelioration of Alloxan Induced Diabetes mellitus and Oxidative Stress in Rats by Oil of <i>Eruca sativa</i> Seeds

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