Amelioration of Alloxan Induced Diabetes mellitus and Oxidative Stress in Rats by Oil of <i>Eruca sativa</i> Seeds

Abstract

Clinical research has confirmed the efficacy of several plant extracts in the modulation of oxidative stress associated with diabetes mellitus (DM). Oil of Eruca sativa seeds (ESS) is tried for prevention and treatment of DM induced experimentally by alloxan injection. A single dose of alloxan (100 mg/kg) produced a decrease in insulin level, hyperglycemia, elevated total lipids, triglycerides and cholesterol, decreased high-density lipoprotein and hepatic glycogen contents and elevated hepatic glucose-6-phosphatase activity. Concurrent with these changes, there was an increase in the concentration of malondialdehyde and 4-hydroxynonenal in the liver. This oxidative stress was related to a decreased glutathione (GSH) content and superoxide dismutase activity in the liver of alloxan-diabetic rats. ESS oil (0.06 ml/kg) on its own increased significantly hepatic GSH. Daily oral administration of ESS oil 2 weeks before or after diabetes induction ameliorated hyperglycemia, improved lipid profile, blunted the increase in malondialdehyde and 4-hydroxynonenal and stimulated the GSH production in the liver of alloxan-treated rats. We suggested that ESS oil could be used as antidiabetic complement in case of DM. This may be related to its antioxidative properties and to the increase in hepatic GSH.

Comparative Proximate Composition and Hypoglycemic Properties of Three Medicinal Plants (Verononia amygdalina, Azadirachta indica and Moringa oleifera).

“…In furtherance to these findings, Ngozi et al, [74] posited that the glycemic control mechanism of G. latifolium could be linked to the capability of the extract to increase cellular ratio of reduced glutathione to oxidized glutathione (GSH/GSSG). Thus, their findings were in concord with previously reported tendency of G. latifolium [75] and oil of Eruca sativa seeds [76] to ameliorate oxidative stress associated with the diabetic state.…”

Antioxidant Status of Liver and Kidney Homogenates from Hyperglycemic Rats Administered with Single and Combinatorial Herbal Formulations

“…Accordingly, the results of the present investigations have shown that GSH/GSSG ratio is an important and reliable biomarker for hyperglycemia and for ascertaining the efficacy of herbal remedies for alleviation of hyperglycemia and amelioration of tissue oxidative stress. The present findings corroborated the reports of El-Missiry and El Gindy, 28 in which they noted that daily oral administration of Eruca sativa seeds oil for 2 weeks stimulated and increased hepatic GSH production of alloxan-treated rats.Zitka et al, 29 used the redox status expressed as GSH:GSSG as a biomarker for ascertaining the level of oxidative stress in paediatric tumor patients, which was similar to earlier reported Murakam et al, 30 on impairment of glutathione metabolism in erythrocytes of patients suffering from DM.…”

Ethnobotanical research of medicinal plants in Mihalgazi (Eskişehir, Turkey)

“…Eruca sativa Miller (Brassicaceae) was used to treat diabetes, ulcer, kidney diseases and high cholesterol. Antidiabetic, antiulcer, renal protective and hypocholesterolemic activity of E.sativa were demonstrated (El-Missiry & El Gindy 2000;El-gengaihi et al 2004;Alam et al 2007;Khan & Khan 2014). Ecballium elaterium (L.) A.…”